Rabbit SPHK1 Antibody | anti-SPHK1 antibody

SPHK1 Antibody

Cat. Num. AAA8502404

• Gene Names: SPHK1; SPHK
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: SPHK1; SPHK1 Antibody; PHK1; SPHK; anti-SPHK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 384

• Applicable Applications for anti-SPHK1 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human SPHK1
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various celllines, using SPHK1 antibody.)

Related Product Information for anti-SPHK1 antibody

Sphingosine kinases (SPHKs) catalyze the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra-and extracellular functions. Together with other sphingolipid metobolizing enzymes, SPHKs regulate the balance of the lipid mediators, ceramide, sphingosine, and S1P (1-4). Two distinct SPHK isoforms, SPHK1 and SPHK2, have been cloned and characterized (5, 6). SPHK1 and SPHK2 are highly conserved and diverselly expressed (7, 8). The SPHKs are activated by G-protein-coupled receptors, receptor tyrosine kinases, immunoglobulin receptors, cytokines, and other stimuli (9-12). The molecular mechanisms by which SPHK1 and SPHK2 are specifically regulated are complex and only partially understood.

Product Categories/Family for anti-SPHK1 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Hait, N.C. et al. (2006) Biochim Biophys Acta 1758, 2016-26. 2. Xia, P. et al. (2000) Curr Biol 10, 1527-30. 3. Hannun, Y.A. et al. (2001) Biochemistry 40, 4893-903. 4. Futerman, A.H. and Riezman, H. (2005) Trends Cell Biol 15, 312-8. 5. Kohama, T. et al. (1998) J Biol Chem 273, 23722-8. 6. Liu, H. et al. (2000) J Biol Chem 275, 19513-20. 7. Liu, H. et al. (2002) Prog Nucleic Acid Res Mol Biol 71, 493-511. 8. Spiegel, S. and Milstien, S. (2003) Nat Rev Mol Cell Biol 4, 397-407. 9. Alemany, R. et al. (2007) Naunyn Schmiedebergs Arch Pharmacol 374, 413-28. 10. Saba, J.D. and Hla, T. (2004) Circ Res 94, 724-34. 11. Anliker, B. and Chun, J. (2004) J Biol Chem 279, 20555-8. 12. Wattenberg, B.W. et al. (2006) J Lipid Res 47, 1128-39.

NCBI and Uniprot Product Information

• NCBI GI #: 217272883
• NCBI GeneID: 8877
• NCBI Accession #: NP_001136073.1
• NCBI GenBank Nucleotide #: NM_001142601.1
• UniProt Accession #: Q9NYA1; Q8N632; Q96GK1; Q9HD92; Q9NY70; Q9NYL3
• Molecular Weight: 43kDa
• NCBI Official Full Name: sphingosine kinase 1 isoform 3
• NCBI Official Synonym Full Names: sphingosine kinase 1
• NCBI Official Symbol: SPHK1
• NCBI Official Synonym Symbols: SPHK
• NCBI Protein Information: sphingosine kinase 1
• UniProt Protein Name: Sphingosine kinase 1
• UniProt Gene Name: SPHK1
• UniProt Synonym Gene Names: SPHK; SPK; SK 1; SPK 1

NCBI Description

The protein encoded by this gene catalyzes the phosphorylation of sphingosine to form sphingosine-1-phosphate (S1P), a lipid mediator with both intra- and extracellular functions. Intracellularly, S1P regulates proliferation and survival, and extracellularly, it is a ligand for cell surface G protein-coupled receptors. This protein, and its product S1P, play a key role in TNF-alpha signaling and the NF-kappa-B activation pathway important in inflammatory, antiapoptotic, and immune processes. Phosphorylation of this protein alters its catalytic activity and promotes its translocation to the plasma membrane. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2017]

Uniprot Description

Catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions. Also acts on D-erythro-sphingosine and to a lesser extent sphinganine, but not other lipids, such as D,L-threo-dihydrosphingosine, N,N-dimethylsphingosine, diacylglycerol, ceramide, or phosphatidylinositol.