SORD recombinant protein

Recombinant Human SORD protein

Cat. Num. AAA1561999

• Gene Names: SORD; SORD1; HEL-S-95n
• Purity: >90% as determined by SDS-PAGE
• Synonyms: SORD; Recombinant Human SORD protein; Sorbitol dehydrogenase; SDH; (R; R)-butanediol dehydrogenase; L-iditol 2-dehydrogenase; Polyol dehydrogenase; Ribitol dehydrogenase; RDH; Xylitol dehydrogenase; XDH; SORD recombinant protein

• Host: E Coli
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized. Lyophilized from PBS pH 7.4, 0.2% NLS, 5% Trehalose, 5% Mannose, 1mM EDTA.

• Species: Homo sapiens (Human)
• Protein Construction: A DNA sequence encoding the human SORD (Ala98-Gln355) was fused with His tag
• Reconstitution: Reconstitute in sterile water for a stock solution.
• Preparation and Storage: Store at 2 to 8 degree C for one week. Store at -20 to -80 degree C for twelve months from the date of receipt. Use a manual defrost freezer and avoid repeated freeze thaw cycles.; In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Related Product Information for SORD recombinant protein

Polyol dehydrogenase that catalyzes the reversible NAD+-dependent oxidation of various sugar alcohols. Is mostly active with D-sorbitol (D-glucitol), L-threitol, xylitol and ribitol as substrates, leading to the C2-oxidized products D-fructose, L-erythrulose, D-xylulose, and D-ribulose, respectively. Is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism. The polyol pathway is believed to be involved in the etiology of diabetic complications, such as diabetic neuropathy and retinopathy, induced by hyperglycemia. May play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility. May have a more general function in the metabolism of secondary alcohols since it also catalyzes the stereospecific oxidation of (2R, 3R)-2, 3-butanediol. To a lesser extent, can also oxidize L-arabinitol, galactitol and D-mannitol and glycerol in vitro. Oxidizes neither ethanol nor other primary alcohols. Cannot use NADP+ as the electron acceptor.

Product Categories/Family for SORD recombinant protein

Protein

NCBI and Uniprot Product Information

• NCBI GI #: 292495088
• NCBI GeneID: 6652
• UniProt Accession #: Q00796; Q16682; Q9UMD6; B2R655; B7Z3A6; J3JZZ5
• Molecular Weight: 357
• NCBI Official Full Name: Sorbitol dehydrogenase
• NCBI Official Synonym Full Names: sorbitol dehydrogenase
• NCBI Official Symbol: SORD
• NCBI Official Synonym Symbols: SORD1; HEL-S-95n
• NCBI Protein Information: sorbitol dehydrogenase; L-iditol 2-dehydrogenase; epididymis secretory sperm binding protein Li 95n
• UniProt Protein Name: Sorbitol dehydrogenase
• Protein Family: Sorbitol-6-phosphate 2-dehydrogenase
• UniProt Gene Name: SORD
• UniProt Entry Name: DHSO_HUMAN

NCBI Description

Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]

Uniprot Description

SORD: Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. Belongs to the zinc-containing alcohol dehydrogenase family.

Protein type: EC 1.1.1.14; Oxidoreductase; Carbohydrate Metabolism - fructose and mannose

Chromosomal Location of Human Ortholog: 15q15.3

Cellular Component: extracellular space; membrane; mitochondrial membrane

Molecular Function: zinc ion binding; L-iditol 2-dehydrogenase activity; carbohydrate binding; NAD binding

Biological Process: sperm motility; L-xylitol metabolic process; L-xylitol catabolic process; glucose metabolic process; fructose biosynthetic process; sorbitol catabolic process