SORD recombinant protein

Recombinant SORD protein

Cat. Num. AAA389339

• Gene Names: SORD; SORD1; HEL-S-95n
• Synonyms: SORD; Recombinant SORD protein; Sorbitol Dehydrogenase; L-iditol 2-dehydrogenase; SORD1; SORD recombinant protein

• Host: E Coli
• Form/Format: Recombinant SORD protein is supplied in 25mM Tris-HCl 7.4, 500mM NaCl, and 5% glycerol.

• Protein Species: Human
• Tag: His-Tag
• Notes: This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
• Dry Ice Shipment: Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
• Preparation and Storage: Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.; Shipping Temp: Dry Ice

SDS-Page

(Recombinant SORD protein gel 10% SDS-PAGE with Coomassie blue staining MW: 41.9kDa Purity: >90%)

Related Product Information for SORD recombinant protein

Short Description: Full-length SORD protein (accession number NP_003095.2) was expressed in E Coli with an N-terminal 6xHis-tag. The molecular weight of the protein is 41.9 kDa.

Background: SORD (Sorbitol Dehydrogenase), also called as RDH, SDH or XDH, is a polyol dehydrogenase that catalyzes the reversible NAD(+)-dependent oxidation of various sugar alcohols. It is mostly active with D-sorbitol (D-glucitol), L-threitol, xylitol and ribitol as substrates, leading to the C2-oxidized products D-fructose, L-erythrulose, D-xylulose, and D-ribulose, respectively. SORD is a key enzyme in the polyol pathway that interconverts glucose and fructose via sorbitol, which constitutes an important alternate route for glucose metabolism. The polyol pathway is believed to be involved in the etiology of diabetic complications, such as diabetic neuropathy and retinopathy, induced by hyperglycemia. It also may play a role in sperm motility by using sorbitol as an alternative energy source for sperm motility. Besides, SORD may have a more general function in the metabolism of secondary alcohols since it also catalyzes the stereospecific oxidation of (2R,3R)-2,3-butanediol. NADP(+) cannot be used as the electron acceptor for SORD dehydrogenase activity.

Product Categories/Family for SORD recombinant protein

Recombinant Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 156627571
• NCBI GeneID: 6652
• NCBI Accession #: NP_003095.2
• NCBI GenBank Nucleotide #: NM_003104.5
• Molecular Weight: 10,812 Da
• NCBI Official Full Name: sorbitol dehydrogenase
• NCBI Official Synonym Full Names: sorbitol dehydrogenase
• NCBI Official Symbol: SORD
• NCBI Official Synonym Symbols: SORD1; HEL-S-95n
• NCBI Protein Information: sorbitol dehydrogenase; L-iditol 2-dehydrogenase; epididymis secretory sperm binding protein Li 95n
• Protein Family: Sorbitol-6-phosphate 2-dehydrogenase

NCBI Description

Sorbitol dehydrogenase (SORD; EC 1.1.1.14) catalyzes the interconversion of polyols and their corresponding ketoses, and together with aldose reductase (ALDR1; MIM 103880), makes up the sorbitol pathway that is believed to play an important role in the development of diabetic complications (summarized by Carr and Markham, 1995 [PubMed 8535074]). The first reaction of the pathway (also called the polyol pathway) is the reduction of glucose to sorbitol by ALDR1 with NADPH as the cofactor. SORD then oxidizes the sorbitol to fructose using NAD(+) cofactor.[supplied by OMIM, Jul 2010]