Mouse Synaptosomal-associated protein 25 ELISA Kit | Snap25 elisa kit

Mouse Synaptosomal-associated protein 25 ELISA Kit

Cat. Num. AAA763008

• Gene Names: SNAP25; SNAP-25
• Reactivity: Mouse
• Synonyms: Synaptosomal-associated protein 25; Mouse Synaptosomal-associated protein 25 ELISA Kit; Snap25/Synaptosomal-associated protein 25/SNAP-25/Super protein/SUP/Synaptosomal-associated 25 kDa protein/SNAP/RIC4/SEC9/SNAP/RIC-4/SNAP-25; Snap25 elisa kit

• Reactivity: Mouse
• Sequence Length: 206

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.312-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Snap25 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Snap25 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Snap25 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Snap25 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Snap25 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 115495867
• NCBI GeneID: 540853
• NCBI Accession #: NP_001069714.1
• NCBI GenBank Nucleotide #: NM_001076246.1
• Molecular Weight: 23,315 Da
• NCBI Official Full Name: synaptosomal-associated protein 25
• NCBI Official Symbol: SNAP25
• NCBI Official Synonym Symbols: SNAP-25
• NCBI Protein Information: synaptosomal-associated protein 25
• UniProt Protein Name: Synaptosomal-associated protein 25
• Protein Family: Synaptosomal-associated protein
• UniProt Gene Name: SNAP25
• UniProt Synonym Gene Names: SNAP-25
• UniProt Entry Name: SNP25_BOVIN

Uniprot Description

t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Modulates the gating characteristics of the delayed rectifier voltage-dependent potassium channel KCNB1 in pancreatic beta cells ().