Mouse TDP43 Monoclonal Antibody | anti-TDP43 antibody

TDP43 Antibody

Cat. Num. AAA415162

• Applications: Immunofluorescence, Western Blot
• Purity: Affinity purified
• Synonyms: TDP43; Monoclonal Antibody; TDP43 Antibody; anti-TDP43 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Human, cow, pig, mouse, rat and other mammals.
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)

• Applicable Applications for anti-TDP43 antibody: Immunofluorescence (IF), Western Blot (WB)
• Application Notes: 1:500 and higher for immunofluorescence, 1:2500 and lower for western blotting.
• Immunogen: TDP43 was originally identified as a protein which binds to the "transactivation response" (TAR) sequence found in the long terminal repeat of the HIV-1 virus genome (1). UV cross-linking of HeLa cell extract revealed a 43kDa protein which was cloned and sequenced and shown to contain a two copies of the ~90 amino acid RRM domain. RRM is an acronym for RNA Recognition Motif, and this domain is found in many proteins which bind single stranded RNA and some which bind single stranded DNA. It also has a single Zinc Finger domain of the ZnF_RBZ subtype found in Ran binding proteins. Ran is a small G protein related to p21-Ras which regulates the import and export of proteins to the nucleus. The protein is frequently refered to by the acronym TDP43 corresponds to "TAR DNA binding protein of molecular weight 43kDa", although the Human Genome Organization (HUGO) Gene Nomenclature Committee (HGNC) name is TARDBP. Northern blots showed that the protein is ubiquitous in tissue expression. Much interest has been focused on TDP43 recently due to its association with the inclusions seen in frontotemporal lobar degeneration and Amyotrophic Lateral Sclerosis (2). The protein is present in these inclusions in a partially degraded, hyperphosphorylated and ubiquitinated form. For a recent review of the role in TDP43 in various neurological disorders see reference 3. This antibody has been used in a recent peer reviewed publication, showing that it works well on western blots and on histological sections (4). The HGNC name for this protein is TARDBP.
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Testing Data #2

Related Product Information for anti-TDP43 antibody

TDP43 is a The TARDBP gene encodes the 43-kD TAR DNA-binding protein, which was originally identified as a transcriptional repressor that binds to TAR DNA of human immunodeficiency virus type 1. It is also involved in regulation of gene expression and splicing (summary by Benajiba et al., 2009).

References

1. Ou SH, Wu F, Harrich D, Garca-Martnez LF and Gaynor RB. Cloning and characterization of a novel cellular protein, TDP-43, that binds to human immunodeficiency virus type 1 TAR DNA sequence motifs. J Virol. 69:3584-96 (1995).2. Neumann, M.; Sampathu, D. M.; Kwong, L. K.; Truax, A. C.; Micsenyi, M. C.; Chou, T. T.; Bruce, J.; Schuck, T.; Grossman, M.; Clark, C. M.; McCluskey, L. F.; Miller, B. L.; Masliah, E.; Mackenzie, I. R.; Feldman, H.; Feiden, W.; Kretzschmar, H. A.; Trojanowski, J. Q.; Lee, V. M.-Y. Ubiquitinated TDP-43 in frontotemporal lobar degeneration and amyotrophic lateral sclerosis. Science 314:130-133 (2006).3. Forman MS, Trojanowski JQ and Lee VM-Y. TDP-43: a novel neurodegenerative proteinopathy. Current Opinions in Neurobiology 17:548-55 (2007).4. Wilson AC, Dugger BN, Dickson DW and Wang DS. TDP-43 in aging and Alzheimer's disease - a review. Int J Clin Exp Pathol. 4:147-55 (2011).