Human Synaptosomal Associated Protein 25kDa (SNAP25) ELISA Kit | SNAP25 elisa kit

Human Synaptosomal Associated Protein 25kDa (SNAP25) ELISA Kit

Cat. Num. AAA2022159

• Gene Names: SNAP25; RIC4; SEC9; SNAP; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
• Reactivity: Human
• Synonyms: Synaptosomal Associated Protein 25kDa (SNAP25); Human Synaptosomal Associated Protein 25kDa (SNAP25) ELISA Kit; SNAP25 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Synaptosomal Associated Protein 25kDa (SNAP25).; No significant cross-reactivity or interference between Synaptosomal Associated Protein 25kDa (SNAP25) and analogues was observed.
• Sequence Length: 206

• Assay Type: Double-antibody Sandwich
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Synaptosomal Associated Protein 25kDa (SNAP25) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Synaptosomal Associated Protein 25kDa (SNAP25) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SNAP25 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Synaptosomal Associated Protein 25kDa (SNAP25). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Synaptosomal Associated Protein 25kDa (SNAP25). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Synaptosomal Associated Protein 25kDa (SNAP25), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Synaptosomal Associated Protein 25kDa (SNAP25) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for SNAP25 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 18765735
• NCBI GeneID: 6616
• NCBI Accession #: NP_570824.1
• NCBI GenBank Nucleotide #: NM_130811.2
• UniProt Accession #: P60880; P13795; P36974; P70557; P70558; Q53EM2; Q5U0B5; Q8IXK3; Q96FM2; B2RAU4; D3DW16; D3DW17
• Molecular Weight: 23,315 Da
• NCBI Official Full Name: synaptosomal-associated protein 25 isoform SNAP25B
• NCBI Official Synonym Full Names: synaptosomal-associated protein, 25kDa
• NCBI Official Symbol: SNAP25
• NCBI Official Synonym Symbols: RIC4; SEC9; SNAP; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
• NCBI Protein Information: synaptosomal-associated protein 25; SUP; super protein; resistance to inhibitors of cholinesterase 4 homolog
• UniProt Protein Name: Synaptosomal-associated protein 25
• Protein Family: Synaptosomal-associated protein
• UniProt Gene Name: SNAP25
• UniProt Synonym Gene Names: SNAP; SNAP-25; SUP
• UniProt Entry Name: SNP25_HUMAN

NCBI Description

Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SNAP-25: a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Part of the SNARE core complex containing SNAP25, VAMP2 and syntaxin 1A. Associates with proteins involved in vesicle docking and membrane fusion. Two alternatively spliced isoforms have been described.

Protein type: Vesicle

Chromosomal Location of Human Ortholog: 20p12-p11.2

Cellular Component: presynaptic membrane; SNARE complex; neuron projection; growth cone; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; trans-Golgi network; cell junction; cytosol; vesicle

Molecular Function: SNAP receptor activity; protein binding; syntaxin-1 binding; calcium-dependent protein binding

Biological Process: synaptic vesicle fusion to presynaptic membrane; synaptic transmission; synaptic vesicle exocytosis; glutamate secretion; neurotransmitter secretion; energy reserve metabolic process; neurotransmitter uptake; pathogenesis; synaptic vesicle priming; regulation of insulin secretion; synaptic vesicle docking during exocytosis

Disease: Myasthenic Syndrome, Congenital, 18