Human Cell death activator CIDE-3, CIDEC ELISA Kit | CIDEC elisa kit

Human Cell death activator CIDE-3, CIDEC ELISA Kit

Cat. Num. AAA1605961

• Gene Names: CIDEC; CIDE3; FPLD5; FSP27; CIDE-3
• Reactivity: Human
• Synonyms: Cell death activator CIDE-3; CIDEC; Human Cell death activator CIDE-3; CIDEC ELISA Kit; CIDEC elisa kit

• Reactivity: Human
• Sequence Length: 164

• Samples: Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 20-3800ng/L
• Sensitivity: 9.46ng/L
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CIDEC elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Cell death activator CIDE-3 (also known as CIDEC) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human CIDEC antibody. CIDEC present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human CIDEC Antibody is added and binds to CIDEC in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CIDEC antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human CIDEC. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 1007383418
• NCBI GeneID: 63924
• NCBI GenBank Nucleotide #: NP_001308073.1
• UniProt Accession #: Q96AQ7
• NCBI Official Full Name: cell death activator CIDE-3 isoform 4
• NCBI Official Synonym Full Names: cell death inducing DFFA like effector c
• NCBI Official Symbol: CIDEC
• NCBI Official Synonym Symbols: CIDE3; FPLD5; FSP27; CIDE-3
• NCBI Protein Information: cell death activator CIDE-3
• UniProt Protein Name: Cell death activator CIDE-3
• Protein Family: Cell death activator
• UniProt Gene Name: CIDEC
• UniProt Synonym Gene Names: FSP27
• UniProt Entry Name: CIDEC_HUMAN

NCBI Description

This gene encodes a member of the cell death-inducing DNA fragmentation factor-like effector family. Members of this family play important roles in apoptosis. The encoded protein promotes lipid droplet formation in adipocytes and may mediate adipocyte apoptosis. This gene is regulated by insulin and its expression is positively correlated with insulin sensitivity. Mutations in this gene may contribute to insulin resistant diabetes. A pseudogene of this gene is located on the short arm of chromosome 3. Alternatively spliced transcript variants that encode different isoforms have been observed for this gene. [provided by RefSeq, Dec 2010]

Uniprot Description

CIDEC: May act as a CEBPB coactivator in white adipose tissue to control the expression of a subset of CEBPB downstream target genes, including SOCS1, SOCS3, TGFB1, TGFBR1, ID2 and XDH. Binds to lipid droplets and regulates their enlargement, thereby restricting lipolysis and favoring storage. At focal contact sites between lipid droplets, promotes directional net neutral lipid transfer from the smaller to larger lipid droplets. The transfer direction may be driven by the internal pressure difference between the contacting lipid droplet pair. When overexpressed in preadipocytes, induces apoptosis or increases cell susceptibility to apoptosis induced by serum deprivation or TGFB treatment. As mature adipocytes, that express high CIDEC levels, are quite resistant to apoptotic stimuli, the physiological significance of its role in apoptosis is unclear. In omental adipose tissue of obese patients matched for BMI, expression levels tend to correlate with insulin sensitivity. Expression is increased 2-3 fold in the group of patients with high insulin sensitivity, compared to the insulin- resistant group. This observation is consistent with the idea that triglyceride storage in adipocytes plays an important role in sequestering triglycerides and fatty acids away from the circulation and peripheral tissues, thus enhancing insulin sensitivity in liver and muscle. This effect is not significant in subcutaneous adipose tissue (PubMed:18509062). In subcutaneous adipose tissue of diabetic patients, tends to negatively correlate with body mass index and total fat mass, independently of insulin sensitivity (PubMed:18334488). 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis

Chromosomal Location of Human Ortholog: 3p25.3

Cellular Component: cytosol; lipid particle

Biological Process: apoptosis; regulation of apoptosis

Disease: Lipodystrophy, Familial Partial, Type 5