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SMPDL3A recombinant protein
SMPDL3A Protein, Mouse, Recombinant (His Tag)
Gene Names
Smpdl3a; ASM3A; ASML3; ASML3A; AI529588; 0610010C24Rik
Purity
>95% as determined by SDS-PAGE
Synonyms
SMPDL3A; SMPDL3A Protein; Mouse; Recombinant (His Tag); Mouse ASM3A/SMPDL3A Protein (His Tag); 0610010C24Rik Protein; AI529588 Protein; ASM3A Protein; ASML3 Protein; ASML3A Protein; sphingomyelin phosphodiesterase; acid-like 3A; SMPDL3A recombinant protein
Host
HEK293 Cells
Purity/Purification
>95% as determined by SDS-PAGE
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Sequence
Met1-Leu445
Species
Mouse
Endotoxin
<1.0EU per ug protein as determined by the LAL method.
Predicted N Terminal
Val 23
Tag
C-His
Protein Construction
A DNA sequence encoding the mouse Smpdl3a (NP_065586.3)(Met1-Leu445) was expressed with a polyhistidine tag at the C-terminus.
Reconstitution
A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.
They are shipped out with blue ice.
SDS-Page
SDS-Page
Related Product Information for SMPDL3A recombinant protein
Background: SMPDL3A gene is a novel liver X receptor (LXR) -regulated gene, with an LXR response element within its promoter. The induction of SMPDL3A is LXR-dependent and is restricted to human blood cells with no induction observed in mouse cellular systems. LXR alpha and LXRbeta function as physiological sensors of cholesterol metabolites (oxysterols), regulating key genes involved in cholesterol and lipid metabolism. LXRs have been extensively studied in both human and rodent cell systems, revealing their potential therapeutic value in the contexts of atherosclerosis and inflammatory diseases. The LXR genome landscape has been investigated in murine macrophages but not in human THP-1 cells, which represent one of the frequently used monocyte/macrophage cell systems to study immune responses.
References
Wright KO, et al. (2002) Increased expression of the acid sphingomyelinase-like protein ASML3a in bladder tumors. J Urol. 168(6):2645-9. Strausberg RL, et al. (2002) Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences. Proc Natl Acad Sci. 99(26):16899-903. Mungall AJ, et al. (2003) The DNA sequence and analysis of human chromosome 6. Nature. 425(6960):805-11.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
49,858 Da
NCBI Official Full Name
acid sphingomyelinase-like phosphodiesterase 3a
NCBI Official Synonym Full Names
sphingomyelin phosphodiesterase, acid-like 3A
NCBI Official Symbol
Smpdl3a
NCBI Official Synonym Symbols
ASM3A; ASML3; ASML3A; AI529588; 0610010C24Rik
NCBI Protein Information
acid sphingomyelinase-like phosphodiesterase 3a; ASM-like phosphodiesterase 3a
UniProt Protein Name
Acid sphingomyelinase-like phosphodiesterase 3a
Protein Family
UniProt Gene Name
Smpdl3a
UniProt Synonym Gene Names
Asml3a; ASM-like phosphodiesterase 3a
UniProt Entry Name
ASM3A_MOUSE
Uniprot Description
SMPDL3A: Belongs to the acid sphingomyelinase family.
Protein type: EC 3.1.4.-; Secreted; Phosphodiesterase; Secreted, signal peptide
Cellular Component: extracellular space; extracellular region
Molecular Function: 3',5'-cyclic-AMP phosphodiesterase activity; cGMP-inhibited cyclic-nucleotide phosphodiesterase activity; calmodulin-dependent cyclic-nucleotide phosphodiesterase activity; photoreceptor cyclic-nucleotide phosphodiesterase activity; sphingomyelin phosphodiesterase activity; hydrolase activity; hydrolase activity, acting on glycosyl bonds; cGMP-stimulated cyclic-nucleotide phosphodiesterase activity; cyclic-nucleotide phosphodiesterase activity
Biological Process: metabolic process; sphingomyelin catabolic process
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Product Notes
The SMPDL3A smpdl3a (Catalog #AAA8123233) is a Recombinant Protein produced from HEK293 Cells and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: Met1-Leu44 5. It is sometimes possible for the material contained within the vial of "SMPDL3A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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