Western Blot (WB)
(SH3RF2 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SH3RF2 antibody detected the SH3RF2 protein (arrow).)
Rabbit anti-Human SH3RF2 Polyclonal Antibody | anti-SH3RF2 antibody
SH3RF2, ID (SH3RF2, PPP1R39, RNF158, Putative E3 ubiquitin-protein ligase SH3RF2, Heart protein phosphatase 1-binding protein, Protein phosphatase 1 regulatory subunit 39, RING finger protein 158, SH3 domain-containing RING finger protein 2) (APC)
Gene Names
SH3RF2; HEPP1; POSHER; RNF158; PPP1R39
Reactivity
Human
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
SH3RF2; Polyclonal Antibody; ID (SH3RF2; PPP1R39; RNF158; Putative E3 ubiquitin-protein ligase SH3RF2; Heart protein phosphatase 1-binding protein; Protein phosphatase 1 regulatory subunit 39; RING finger protein 158; SH3 domain-containing RING finger protein 2) (APC); SH3 domain-containing RING finger protein 2; anti-SH3RF2 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Applicable Applications for anti-SH3RF2 antibody
Western Blot (WB), FLISA
Application Notes
WB: 1:100-500
FLISA: 1:1,000
Applications are based on unconjugated antibody.
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
SH3RF2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 456-483 amino acids from the Central region of human SH3RF2.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Western Blot (WB)
(SH3RF2 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SH3RF2 antibody detected the SH3RF2 protein (arrow).)
Western Blot (WB)
(SH3RF2 Antibody (Center) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the SH3RF2 antibody detected the SH3RF2 protein (arrow).)
Related Product Information for anti-SH3RF2 antibody
SH3RF2 may be a E3 ubiquitin-protein ligase (Potential).
Product Categories/Family for anti-SH3RF2 antibody
References
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010): Chen, C.Y., et al. Biochem. Biophys. Res. Commun. 391(1):698-702(2010). Voss, M., et al. BMC Immunol. 10, 53 (2009).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
79,320 Da
NCBI Official Full Name
putative E3 ubiquitin-protein ligase SH3RF2
NCBI Official Synonym Full Names
SH3 domain containing ring finger 2
NCBI Official Symbol
SH3RF2
NCBI Official Synonym Symbols
HEPP1; POSHER; RNF158; PPP1R39
NCBI Protein Information
putative E3 ubiquitin-protein ligase SH3RF2; RING finger protein 158; POSH-eliminating RING protein; SH3 domain-containing RING finger protein 2; heart protein phosphatase 1-binding protein; protein phosphatase 1 regulatory subunit 39; protein phosphatase
UniProt Protein Name
Putative E3 ubiquitin-protein ligase SH3RF2
Protein Family
UniProt Gene Name
SH3RF2
UniProt Synonym Gene Names
PPP1R39; RNF158; HEPP1
UniProt Entry Name
SH3R2_HUMAN
Uniprot Description
SH3RF2: Inhibits PPP1CA phosphatase activity. May be a E3 ubiquitin-protein ligase (Potential). May play a role in cardiac function. Belongs to the SH3RF family. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Ligase; EC 6.3.2.-; Ubiquitin ligase; Ubiquitin conjugating system
Chromosomal Location of Human Ortholog: 5q32
Cellular Component: nucleoplasm
Molecular Function: protein binding; zinc ion binding; protein phosphatase inhibitor activity; protein phosphatase 1 binding; phosphatase binding; ligase activity
Biological Process: protein ubiquitination
Similar Products
Product Notes
The SH3RF2 sh3rf2 (Catalog #AAA6347286) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SH3RF2, ID (SH3RF2, PPP1R39, RNF158, Putative E3 ubiquitin-protein ligase SH3RF2, Heart protein phosphatase 1-binding protein, Protein phosphatase 1 regulatory subunit 39, RING finger protein 158, SH3 domain-containing RING finger protein 2) (APC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SH3RF2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SH3RF2 sh3rf2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SH3RF2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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