Cortisol Binding Globulin Recombinant Protein | SerpinA6 recombinant protein

Cortisol Binding Globulin Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122677

• Gene Names: Serpina6; Cbg
• Purity: >98% as determined by SDS-PAGE
• Synonyms: Cortisol Binding Globulin; Cortisol Binding Globulin Protein; Mouse; Recombinant (His Tag); Mouse SerpinA6/CBG Protein (His Tag); AI265318 Protein; AV104445 Protein; Cbg Protein; SerpinA6 Protein; serpin peptidase inhibitor; clade A (alpha-1 antiproteinase; antitrypsin); member 6; SerpinA6 recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >98% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Ala397

• Species: Mouse
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Val 23
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse SERPINA6 (Q06770)(Met 1-Ala 397) was fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for SerpinA6 recombinant protein

Background: Corticosteroid-binding globulin (CBG), also known as SerpinA6, is a non-inhibitory member of the serine proteinase inhibitor (serpin) superfamily. It is the high-affinity transport protein for glucocorticoids in vertebrate blood. CBG is specifically cleaved by this protease at a precise site close to its carboxy-terminus. This induces a conformation change and disrupts the binding between glucocorticoids and CBG, and promotes a significant and local release of glucocorticoids (over 90% of them are bound to CBG in human plasma). In this context, CBG directs glucocorticoids to sites of inflammation, and plays in consequence a crucial role in efficient glucocorticoid action in physiology. The SerpinA6 protein is mainly secreted by the liver. This negative acute phase protein regulates free cortisol levels in the blood and distributes cortisol to its target tissues. SerpinA6 deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo-/hypertension and muscle fatigue. There are three heritable, human CBG gene mutations that can reduce CBG-cortisol binding affinity and/or reduce circulating CBG levels.

References

Seralini GE. (1991) A new role for corticosteroid binding globulin (CBG), member of SERPIN superfamily. C R Seances Soc Biol Fil. 185(6): 500-9.Buss C, et al. (2007) Haploinsufficiency of the SERPINA6 gene is associated with severe muscle fatigue: A de novo mutation in corticosteroid-binding globulin deficiency. J Neural Transm. 114(5): 563-9.Torpy DJ, et al. (2007) Corticosteroid-binding globulin gene polymorphisms: clinical implications and links to idiopathic chronic fatigue disorders. Clin Endocrinol (Oxf). 67(2): 161-7.Braun BC, et al. (2010) Effect of mutations of the human serpin protein corticosteroid-binding globulin on cortisol-binding, thermal and protease sensitivity. J Steroid Biochem Mol Biol. 120(1): 30-7.Lin HY, et al. (2010) Molecular and structural basis of steroid hormone binding and release from corticosteroid-binding globulin. Mol Cell Endocrinol. 316(1): 3-12.

NCBI and Uniprot Product Information

• NCBI GI #: 6680856
• NCBI GeneID: 12401
• NCBI Accession #: NP_031644.1
• NCBI GenBank Nucleotide #: NM_007618.3
• UniProt Accession #: Q06770
• Molecular Weight: 44,769 Da
• NCBI Official Full Name: corticosteroid-binding globulin
• NCBI Official Synonym Full Names: serine (or cysteine) peptidase inhibitor, clade A, member 6
• NCBI Official Symbol: Serpina6
• NCBI Official Synonym Symbols: Cbg
• NCBI Protein Information: corticosteroid-binding globulin
• UniProt Protein Name: Corticosteroid-binding globulin
• Protein Family: Corticosteroid-binding globulin
• UniProt Gene Name: Serpina6
• UniProt Synonym Gene Names: Cbg; CBG

NCBI Description

This gene encodes a protein that belongs to the serpin (serine protease inhibitor) family. The encoded protein is an alpha-globulin with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors. [provided by RefSeq, Sep 2015]

Uniprot Description

Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.