SDS-Page
(Recombinant SIRT4 (25-314) 12.5% SDS-PAGE with Coomassie Blue staining MW: 64 kDa)
SIRT4 recombinant protein
Recombinant SIRT4 (25-314) protein
Purity
Recombinant SIRT4 (25-314) protein that includes amino acids 25 -314 of human SIRT4 protein (accession number NM_012240.2) was expressed in E Coli and contains an N-terminal GST-Tag with a molecular weight of 64 kDa.The purity ofThe protein is > 60% by SD
Synonyms
SIRT4; Recombinant SIRT4 (25-314) protein; SIRT-4; Sirtuin 4; EC 3.5.1; SIR2L4; SIR2-Like 4; SIRT4 recombinant protein
Host
E Coli
Purity/Purification
Recombinant SIRT4 (25-314) protein that includes amino acids 25 -314 of human SIRT4 protein (accession number NM_012240.2) was expressed in E Coli and contains an N-terminal GST-Tag with a molecular weight of 64 kDa.The purity ofThe protein is > 60% by SD
Form/Format
Recombinant SIRT4 (25-314) protein is supplied in 25mM Tris pH8.0, 300mM NaCl, 5% glycerol. Please refer to product insert upon arrival for lot-specific concentration.
Protein Species
Human
Tag
GST-Tag
Notes
This product was manufactured as described in Protein Details. Where possible, We has developed functional or activity assays for recombinant proteins. Additional characterization such as enzyme kinetic activity assays, inhibitor screening or other biological activity assays may not have been performed for every product. All available data for a given product is shown on the lot-specific Technical Data Sheet.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant SIRT4 (25-314) 12.5% SDS-PAGE with Coomassie Blue staining MW: 64 kDa)
SDS-Page
(Recombinant SIRT4 (25-314) 12.5% SDS-PAGE with Coomassie Blue staining MW: 64 kDa)
Related Product Information for SIRT4 recombinant protein
Short Description: Recombinant SIRT4 (25-314) protein includes amino acids 25 -314 of human SIRT4 protein (accession number NM_012240.2); it was expressed in E Coli and contains an N-terminal GST-Tag with a molecular weight of 64 kDa. The purity of the protein is > 60% by SDS-PAGE.
Background: SIRT4 (Sirtuin 4) is a member of the SIR2/Sirtuin family of NAD+-dependant deacetylase enzymes. Sirtuin family enzymes can be found in the cytoplasm, mitochondria or nucleus of a cell and are ubiquitously expressed. SIRT4 has multiple enzymatic functions, such as NAD+-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. SIRT4 catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. It inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 catalyzes the transfer of ADP-ribosyl groups onto target proteins, such as mitochondrial GLUD1. It acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1. It also acts as a NAD+-dependent protein deacetylase to mediate deacetylation of Lys-471 of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis.
Background: SIRT4 (Sirtuin 4) is a member of the SIR2/Sirtuin family of NAD+-dependant deacetylase enzymes. Sirtuin family enzymes can be found in the cytoplasm, mitochondria or nucleus of a cell and are ubiquitously expressed. SIRT4 has multiple enzymatic functions, such as NAD+-dependent protein lipoamidase, ADP-ribosyl transferase and deacetylase. SIRT4 catalyzes more efficiently removal of lipoyl- and biotinyl- than acetyl-lysine modifications. It inhibits the pyruvate dehydrogenase complex (PDH) activity via the enzymatic hydrolysis of the lipoamide cofactor from the E2 component, DLAT, in a phosphorylation-independent manner. SIRT4 catalyzes the transfer of ADP-ribosyl groups onto target proteins, such as mitochondrial GLUD1. It acts as a negative regulator of mitochondrial glutamine metabolism by mediating mono ADP-ribosylation of GLUD1. It also acts as a NAD+-dependent protein deacetylase to mediate deacetylation of Lys-471 of MLYCD, inhibiting its activity, thereby acting as a regulator of lipid homeostasis.
Product Categories/Family for SIRT4 recombinant protein
NCBI and Uniprot Product Information
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Molecular weight of 64kDa.
Protein Family
Similar Products
Product Notes
The SIRT4 (Catalog #AAA388234) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SIRT4, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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