Human sialic acid binding Ig-like lectin 8 ELISA Kit | SIGLEC8 elisa kit

Human Sialic acid-binding Ig-like lectin 8, SIGLEC8 ELISA Kit

Cat. Num. AAA919455

• Gene Names: SIGLEC8; SAF2; SIGLEC-8; SIGLEC8L
• Reactivity: Human
• Synonyms: sialic acid binding Ig-like lectin 8; Human Sialic acid-binding Ig-like lectin 8; SIGLEC8 ELISA Kit; Human Sialic acid-binding Ig-like lectin 8 (SIGLEC8) ELISA kit; MGC59785; SAF2; SIGLEC-8; SIGLEC8L; ; SIGLEC8 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human SIGLEC8. No significant cross-reactivity or interference between human SIGLEC8 and analogues was observed.
• Sequence Length: 499

• Samples: Serum, plasma, tissue homogenates, cell lysates
• Detection Range: 0.156 ng/ml -10 ng/mL
• Sensitivity: <0.039 ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SIGLEC8 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SIGLEC8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SIGLEC8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIGLEC8 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIGLEC8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 94538343
• NCBI GeneID: 27181
• NCBI Accession #: NP_055257.2
• NCBI GenBank Nucleotide #: NM_014442.2
• UniProt Accession #: Q9NYZ4; Q7Z728
• Molecular Weight: 54,042 Da
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 8
• NCBI Official Synonym Full Names: sialic acid binding Ig-like lectin 8
• NCBI Official Symbol: SIGLEC8
• NCBI Official Synonym Symbols: SAF2; SIGLEC-8; SIGLEC8L
• NCBI Protein Information: sialic acid-binding Ig-like lectin 8; SAF-2; CDw329; sialoadhesin family member 2
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 8
• Protein Family: Sialic acid-binding Ig-like lectin
• UniProt Gene Name: SIGLEC8
• UniProt Synonym Gene Names: SAF2; Siglec-8; SAF-2
• UniProt Entry Name: SIGL8_HUMAN

NCBI Description

Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010]

Uniprot Description

SIGLEC8: Putative adhesion molecule that mediates sialic-acid dependent binding to cells. Preferentially binds to alpha-2,3- linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Receptor, misc.; Cell adhesion

Chromosomal Location of Human Ortholog: 19q13.33-q13.41

Cellular Component: integral to membrane

Molecular Function: protein binding; transmembrane receptor activity; carbohydrate binding

Biological Process: signal transduction; cell adhesion