Human Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8) ELISA Kit | SIGLEC8 elisa kit

Human Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8) ELISA Kit

Cat. Num. AAA2703698

• Gene Names: SIGLEC8; SAF2; SIGLEC-8; SIGLEC8L
• Reactivity: Human
• Synonyms: Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8); Human Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8) ELISA Kit; SAF2; SIGLEC-8; SIGLEC8L; Sialoadhesin family member 2; SIGLEC8 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8).; No significant cross-reactivity or interference between Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8) and analogues was observed.
• Sequence Length: 431

• Assay Type: Double-antibody Sandwich
• Samples: Cell Lysates
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SIGLEC8 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sialic Acid Binding Ig Like Lectin 8 (SIGLEC8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 94538343
• NCBI GeneID: 27181
• NCBI Accession #: NP_055257.2
• NCBI GenBank Nucleotide #: NM_014442.2
• UniProt Accession #: Q9NYZ4; Q7Z728
• Molecular Weight: 46,434 Da
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 8
• NCBI Official Synonym Full Names: sialic acid binding Ig like lectin 8
• NCBI Official Symbol: SIGLEC8
• NCBI Official Synonym Symbols: SAF2; SIGLEC-8; SIGLEC8L
• NCBI Protein Information: sialic acid-binding Ig-like lectin 8
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 8
• Protein Family: Sialic acid-binding Ig-like lectin
• UniProt Gene Name: SIGLEC8
• UniProt Synonym Gene Names: SAF2; Siglec-8; SAF-2

NCBI Description

Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010]

Uniprot Description

Putative adhesion molecule that mediates sialic-acid dependent binding to red blood cells (PubMed:10856141, PubMed:10625619). Preferentially binds to alpha-2,3-linked sialic acid. Also binds to alpha-2,6-linked sialic acid. The sialic acid recognition site may be masked by cis interactions with sialic acids on the same cell surface (PubMed:10625619). Recognizes simultaneously epitopes having a terminal N-acetylneuraminic acid (sialic acid) and an underlying 6-O-sulfated galactose. Preferentially binds to Gal-6-sulfated sialyl-Lewis X glycan epitopes (PubMed:27357658).