Human Sialic Acid-Binding Ig-Like Lectin 8 (SIGLEC8) ELISA Kit | SIGLEC8 elisa kit

Human Sialic Acid-Binding Ig-Like Lectin 8 (SIGLEC8) ELISA Kit

Cat. Num. AAA9714324

• Gene Names: SIGLEC8; SAF2; SIGLEC-8; SIGLEC8L
• Reactivity: Human
• Synonyms: Sialic Acid-Binding Ig-Like Lectin 8 (SIGLEC8); Human Sialic Acid-Binding Ig-Like Lectin 8 (SIGLEC8) ELISA Kit; SIGLEC8; MGC59785; SAF2; SIGLEC-8; SIGLEC8L; SIGLEC8 elisa kit

• Reactivity: Human
• Specificity: Human Sialic acid-binding Ig-like lectin 8 (SIGLEC8) ELISA Kit has high sensitivity and excellent specificity for detection of Human SIGLEC8. No significant cross-reactivity or interference between Human SIGLEC8 and analogues was observed.
• Sequence Length: 499

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Sialic acid-binding Ig-like lectin 8 (SIGLEC8) Standard Curve.)

Related Product Information for SIGLEC8 elisa kit

Description: This Human Sialic acid-binding Ig-like lectin 8 (SIGLEC8) ELISA Kit employs a two-site sandwich ELISA to quantitate SIGLEC8 in samples. An antibody specific for SIGLEC8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SIGLEC8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SIGLEC8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SIGLEC8 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Floyd et al. (2000) identified a cDNA from an eosinophil cDNA library encoding SIGLEC8. Sequence analysis predicted that like other SIGLECs, the 431-amino acid, type 1 transmembrane protein contains a signal peptide, an N-terminal V-set domain, and 2 C2-set domains, as well as 3 potential N-linked glycosylation sites and a transmembrane region; however, SIGLEC8 has a truncated 47-residue cytoplasmic tail lacking the conserved tyrosine-based motifs. Binding analysis confirmed that SIGLEC8 binds to red blood cell sialic acids with a preference for 3-prime over 6-prime sialyllactose-conjugated polyacrylamide. FACS and immunoprecipitation analyses demonstrated SIGLEC8 expression on eosinophils but not other leukocytes as a 45- and 89-kD dimer.

NCBI and Uniprot Product Information

• NCBI GI #: 94538343
• NCBI GeneID: 27181
• NCBI Accession #: NP_055257
• NCBI GenBank Nucleotide #: NP_055257.2
• UniProt Accession #: Q9NYZ4
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 8 isoform 1
• NCBI Official Synonym Full Names: sialic acid binding Ig like lectin 8
• NCBI Official Symbol: SIGLEC8
• NCBI Official Synonym Symbols: SAF2; SIGLEC-8; SIGLEC8L
• NCBI Protein Information: sialic acid-binding Ig-like lectin 8
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 8
• Protein Family: Sialic acid-binding Ig-like lectin
• UniProt Gene Name: SIGLEC8
• UniProt Synonym Gene Names: SAF2; Siglec-8; SAF-2
• UniProt Entry Name: SIGL8_HUMAN

NCBI Description

Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010]