Human Sialic acid-binding Ig-like lectin 8, SIGLEC8 ELISA Kit | SIGLEC8 elisa kit

Human Sialic acid-binding Ig-like lectin 8, SIGLEC8 ELISA Kit

Cat. Num. AAA1607026

• Gene Names: SIGLEC8; SAF2; SIGLEC-8; SIGLEC8L
• Reactivity: Human
• Synonyms: Sialic acid-binding Ig-like lectin 8; SIGLEC8; Human Sialic acid-binding Ig-like lectin 8; SIGLEC8 ELISA Kit; SIGLEC8 elisa kit

• Reactivity: Human
• Sequence Length: 499

• Samples: Serum, plasma, cell culture supernates, ascites, tissue homogenates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.05-20ng/mL
• Sensitivity: 0.05-20ng/mL
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SIGLEC8 elisa kit

Intended Uses: This sandwich kit is for the accurate quantitative detection of Human Sialic acid-binding Ig-like lectin 8 (also known as SIGLEC8) in serum, plasma, cell culture supernates, Ascites, tissue homogenates or other biological fluids.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with Human SIGLEC8 antibody. SIGLEC8 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated Human SIGLEC8 Antibody is added and binds to SIGLEC8 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated SIGLEC8 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of Human SIGLEC8. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 94538343
• NCBI GeneID: 27181
• NCBI GenBank Nucleotide #: NP_055257.2
• UniProt Accession #: Q9NYZ4
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 8 isoform 1
• NCBI Official Synonym Full Names: sialic acid binding Ig like lectin 8
• NCBI Official Symbol: SIGLEC8
• NCBI Official Synonym Symbols: SAF2; SIGLEC-8; SIGLEC8L
• NCBI Protein Information: sialic acid-binding Ig-like lectin 8
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 8
• Protein Family: Sialic acid-binding Ig-like lectin
• UniProt Gene Name: SIGLEC8
• UniProt Synonym Gene Names: SAF2; Siglec-8; SAF-2
• UniProt Entry Name: SIGL8_HUMAN

NCBI Description

Sialic acid-binding immunoglobulin (Ig)-like lectins, or SIGLECs (e.g., CD33 (MIM 159590)), are a family of type 1 transmembrane proteins each having a unique expression pattern, mostly in hemopoietic cells. SIGLEC8 is a member of the CD33-like subgroup of SIGLECs, which are localized to 19q13.3-q13.4 and have 2 conserved cytoplasmic tyrosine-based motifs: an immunoreceptor tyrosine-based inhibitory motif, or ITIM (see MIM 604964), and a motif homologous to one identified in signaling lymphocyte activation molecule (SLAM; MIM 603492) that mediates an association with SLAM-associated protein (SAP; MIM 300490) (summarized by Foussias et al., 2000 [PubMed 11095983]).[supplied by OMIM, May 2010]