SIGLEC15/CD33L3 recombinant protein

Recombinant Human SIGLEC15/CD33L3 Protein (C-His)

Cat. Num. AAA2557138

• Gene Names: SIGLEC15; CD33L3; HsT1361; SIGLEC-15
• Purity: >95% as determined by reducing SDS-PAGE.
• Synonyms: SIGLEC15/CD33L3; Recombinant Human SIGLEC15/CD33L3 Protein (C-His); Sialic acid-binding Ig-like lectin 15; Siglec-15; CD33 antigen-like 3; CD33L3; SIGLEC15/CD33L3 recombinant protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 mum filtered solution of PBS, 150mMNacl, 0.3%chaps, 5% Trehalose, pH7.4.
• Sequence: Phe20-Thr263
• Sequence Length: 328

• Species: Human
• Endotoxin: < 1.0 EU per mug as determined by the LAL method.
• Fusion Tag: C-His
• Preparation and Storage: Lyophilized protein should be stored at < -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.

Related Product Information for SIGLEC15/CD33L3 recombinant protein

Protein Construction: Recombinant Human SIGLEC15 is produced by our mammalian expression system and the target gene encoding Phe20-Thr263 is expressed with a 6His tag at the C-terminus.

Background: Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO?-terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.

NCBI and Uniprot Product Information

• NCBI GI #: 47106069
• NCBI GeneID: 284266
• NCBI Accession #: NP_998767
• NCBI GenBank Nucleotide #: NP_998767.1
• UniProt Accession #: Q6ZMC9
• Molecular Weight: Molecular Mass: 27.4kDa; Actual Protein Molecular Mass: 30-40kDa
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 15
• NCBI Official Synonym Full Names: sialic acid binding Ig like lectin 15
• NCBI Official Symbol: SIGLEC15
• NCBI Official Synonym Symbols: CD33L3; HsT1361; SIGLEC-15
• NCBI Protein Information: sialic acid-binding Ig-like lectin 15
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 15
• UniProt Gene Name: SIGLEC15
• UniProt Synonym Gene Names: CD33L3; Siglec-15
• UniProt Entry Name: SIG15_HUMAN

Uniprot Description

CD33L3: Binds sialylated glycoproteins. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q12.3

Cellular Component: protein complex; integral to membrane; plasma membrane

Biological Process: regulation of bone resorption; regulation of actin cytoskeleton organization and biogenesis; innate immune response