Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 Active Protein | CD33L3 active protein

Recombinant Human Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-Fc)

Cat. Num. AAA2569034

• Gene Names: SIGLEC15; CD33L3; HsT1361; SIGLEC-15
• Purity: >95% as determined by reducing SDS-PAGE
• Synonyms: Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3; Recombinant Human Sialic acid-binding Ig-like lectin 15/Siglec-15/CD33L3 (C-Fc); Angiopoietin-related protein 4; 425O18-1; Angiopoietin-like protein 4; Fasting-induced adipose factor; Hepatic fibrinogen/angiopoietin-related protein; HFARP; Secreted protein Bk89; Angptl4; Farp; Fiaf; Ng27; CD33L3 active protein

• Host: Human Cells
• Purity/Purification: >95% as determined by reducing SDS-PAGE
• Form/Format: Lyophilized from a 0.2um filtered solution of PBS, 150mM NaCl, 0.3% Chaps, 5% Trehalose, pH7.4.
• Sequence: Phe20-Thr263

• Species: Human
• Tag: C-Fc
• Endotoxin: <1.0EU per ug as determined by the LAL method.
• Protein Construction: Recombinant Human Sialic Acid-binding Ig-like Lectin 15 is produced by our Mammalian expression system and the target gene encoding Phe20-Thr263 is expressed with a Fc tag at the C-terminus.
• Activity: Immobilized Human Siglec-15-Fc at 2ug/ml (100ul/well) can bind Anti-Human Siglec15 mAb. The ED50 of Anti-Human Siglec15 mAb is 16.8ng/ml.
• Preparation and Storage: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80 degree C. Reconstituted protein solution can be stored at 4-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at < -20 degree C for 3 months.; This product is provided as lyophilized powder which is shipped with ice packs.

SDS-Page

Activity

Related Product Information for CD33L3 active protein

Human Siglec-15 is a transmembrane glycoprotein in the Siglec family. Siglecs are type I transmembrane proteins where the NH3+-terminus is in the extracellular space and the COO--terminus is cytosolic. Each Siglec contains an N-terminal V-type immunoglobulin domain (Ig domain) which acts as the binding receptor for sialic acid. These lectins are placed into the group of I-type lectins because the lectin domain is an immunoglobulin fold. All Siglecs are extended from the cell surface by C2-type Ig domains which have no binding activity. Siglecs differ in the number of these C2-type domains. Human Siglec-15 consists of a 244 amino acid (aa) extracellular domain (ECD) with two Ig-like domains, a 21 aa transmembrane segment, and a 44 aa cytoplasmic domain. Siglec-15 function is important for osteoclast formation and TRANCE/RANK Ligand signaling in osteoclasts.

NCBI and Uniprot Product Information

• NCBI GI #: 47106069
• NCBI GeneID: 284266
• NCBI Accession #: NP_998767
• NCBI GenBank Nucleotide #: NM_213602.2
• UniProt Accession #: Q6ZMC9; A8K2Y5; B4DVQ9
• Molecular Weight: 18,086 Da
• NCBI Official Full Name: sialic acid-binding Ig-like lectin 15
• NCBI Official Synonym Full Names: sialic acid binding Ig-like lectin 15
• NCBI Official Symbol: SIGLEC15
• NCBI Official Synonym Symbols: CD33L3; HsT1361; SIGLEC-15
• NCBI Protein Information: sialic acid-binding Ig-like lectin 15; CD33 antigen-like 3; CD33 molecule-like 3
• UniProt Protein Name: Sialic acid-binding Ig-like lectin 15
• UniProt Gene Name: SIGLEC15
• UniProt Synonym Gene Names: CD33L3; Siglec-15
• UniProt Entry Name: SIG15_HUMAN

Uniprot Description

CD33L3: Binds sialylated glycoproteins. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 18q12.3

Cellular Component: protein complex; integral to membrane; plasma membrane

Biological Process: regulation of bone resorption; regulation of actin cytoskeleton organization and biogenesis; innate immune response