Western Blot (WB)
(SIGLEC15 Antibody)
Rabbit SIGLEC15 Polyclonal Antibody | anti-SIGLEC15 antibody
SIGLEC15 Antibody
Gene Names
SIGLEC15; CD33L3; HsT1361; SIGLEC-15
Reactivity
Human, Mouse, Rat
Applications
ELISA, Western Blot, Immunohistochemistry
Purity
SIGLEC15 Antibody is affinity chromatography purified via peptide column.
Synonyms
SIGLEC15; Polyclonal Antibody; SIGLEC15 Antibody; CD33L3; HsT1361; SIGLEC-15; Sialic acid-binding Ig-like lectin 15; CD33 antigen-like 3; Siglec-15; sialic acid binding Ig-like lectin 15; anti-SIGLEC15 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
SIGLEC15 antibody is predicted to not cross-react with other SIGLEC family members.
Purity/Purification
SIGLEC15 Antibody is affinity chromatography purified via peptide column.
Form/Format
Liquid; SIGLEC15 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration
1 mg/mL (varies by lot)
Sequence Length
328
Applicable Applications for anti-SIGLEC15 antibody
ELISA (EIA), Western Blot (WB), Immunohistochemistry-Parrafin (IHC-P)
Application Notes
SIGLEC15 antibody can be used for detection of SIGLEC15 by WB at 1 - 2 ug/mL.
Antibody can also be used for IHC starting at 2.5 ug/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Antibody can also be used for IHC starting at 2.5 ug/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested.
Immunogen
SIGLEC15 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human SIGLEC15.
The immunogen is located within amino acids 50-100 of SIGLEC15.
The immunogen is located within amino acids 50-100 of SIGLEC15.
Preparation and Storage
SIGLEC15 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Western Blot (WB)
(SIGLEC15 Antibody)
Western Blot (WB)
(SIGLEC15 Antibody)
Immunohistochemistry (IHC)
(Immunohistochemistry of SIGLEC15 in human kidney tissue with SIGLEC15 antibody at 2.5 μg/mL.)
Immunohistochemistry (IHC)
(Immunohistochemistry of SIGLEC15 in human kidney tissue with SIGLEC15 antibody at 2.5 μg/mL.)
Related Product Information for anti-SIGLEC15 antibody
SIGLEC15 Antibody: Siglecs are vertebrate cell-surface lectins that recognize sialylated glycans and are involved in many physiological processes, such as glycoprotein turnover, cellular trafficking, and pathogen recognition. Most Siglecs are expressed on cells of the immune system. SIGLEC15 is a type-I transmembrane protein consisting of two immunoglobulin (Ig)-like domains, a transmembrane domain containing a lysine residue, and a short cytoplasmic tail. SIGLEC15 can interact with the activating adaptor molecules DAP12/10. Its activating signaling potential and unique preference for glycan recognition implies that SIGLEC15 may be involved in the immune surveillance of tumors and probably plays a conserved, regulatory role in the immune system of vertebrates.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Predicted MW: 36kDa
Observed MW: 42kDa
Observed MW: 42kDa
NCBI Official Full Name
sialic acid-binding Ig-like lectin 15
NCBI Official Synonym Full Names
sialic acid binding Ig-like lectin 15
NCBI Official Symbol
SIGLEC15
NCBI Official Synonym Symbols
CD33L3; HsT1361; SIGLEC-15
NCBI Protein Information
sialic acid-binding Ig-like lectin 15; CD33 antigen-like 3; CD33 molecule-like 3
UniProt Protein Name
Sialic acid-binding Ig-like lectin 15
Protein Family
UniProt Gene Name
SIGLEC15
UniProt Synonym Gene Names
CD33L3; Siglec-15
UniProt Entry Name
SIG15_HUMAN
Uniprot Description
CD33L3: Binds sialylated glycoproteins. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 18q12.3
Cellular Component: protein complex; integral to membrane; plasma membrane
Biological Process: regulation of bone resorption; regulation of actin cytoskeleton organization and biogenesis; innate immune response
Research Articles on SIGLEC15
Similar Products
Product Notes
The SIGLEC15 siglec15 (Catalog #AAA150636) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SIGLEC15 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's SIGLEC15 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry-Parrafin (IHC-P). SIGLEC15 antibody can be used for detection of SIGLEC15 by WB at 1 - 2 ug/mL.Antibody can also be used for IHC starting at 2.5 ug/mL.
Antibody validated: Western Blot in human samples and Immunohistochemistry in human samples. All other applications and species not yet tested. Researchers should empirically determine the suitability of the SIGLEC15 siglec15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SIGLEC15, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.
Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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