Rabbit anti-Human, Mouse Uridine Monophosphate Kinase 1 Polyclonal Antibody | anti-URK1 antibody

Uridine Monophosphate Kinase 1, NT (Cytidine monophosphokinase 1, UCK 1, Uridine-cytidine kinase 1, Uridine monophosphokinase 1, URK1) (FITC)

Cat. Num. AAA6504851

• Gene Names: CMPK1; CK; CMK; UMK; CMPK; UMPK; UMP-CMPK; RP11-511I2.1
• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: Uridine Monophosphate Kinase 1; Polyclonal Antibody; NT (Cytidine monophosphokinase 1; UCK 1; Uridine-cytidine kinase 1; Uridine monophosphokinase 1; URK1) (FITC); anti-URK1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human UCK. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-URK1 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human UCK (KLH).
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-UCK Pab is used in Western blot to detect UCK in mouse cerebellum tissue lysate (Lane 1) and HepG2 cell lysate (Lane 2).)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-URK1 antibody

Antibodies; Protein Kinases

References

1. Strausberg, R.L., et al., Proc. Natl. Acad. Sci. USA 99(26):16899-16903 (2002). 2. Hu, R.M., et al., Proc. Natl. Acad. Sci. USA 97(17):9543-9548 (2000). 3. Hughes, G.J., et al., Electrophoresis 14(11):1216-1222 (1993). 4. Hochstrasser, D.F., et al., Electrophoresis 13(12):992-1001 (1992). 5. BD Zlatopolskiy, et al. J Nucl Med. 2009; 50: 1895. (Applications: IHC)(E-pub).

NCBI and Uniprot Product Information

• NCBI GI #: 209915597
• NCBI GeneID: 51727
• NCBI Accession #: NP_001129612.1
• NCBI GenBank Nucleotide #: NM_001136140.1
• UniProt Accession #: P30085; Q53GB7; Q5SVZ0; Q96C07; Q9UBQ8; Q9UIA2
• Molecular Weight: 22,222 Da
• NCBI Official Full Name: UMP-CMP kinase isoform b
• NCBI Official Synonym Full Names: cytidine monophosphate (UMP-CMP) kinase 1, cytosolic
• NCBI Official Symbol: CMPK1
• NCBI Official Synonym Symbols: CK; CMK; UMK; CMPK; UMPK; UMP-CMPK; RP11-511I2.1
• NCBI Protein Information: UMP-CMP kinase; dCMP kinase; UMP/CMP kinase; cytidylate kinase; deoxycytidylate kinase; uridine monophosphate kinase; uridine monophosphate/cytidine monophosphate kinase
• UniProt Protein Name: UMP-CMP kinase
• UniProt Gene Name: CMPK1
• UniProt Synonym Gene Names: CMK; CMPK; UCK; UMK; UMPK; CK; dCMP kinase; UMP/CMP kinase; UMP/CMPK
• UniProt Entry Name: KCY_HUMAN

NCBI Description

This gene encodes one of the enzymes required for cellular nucleic acid biosynthesis. This enzyme catalyzes the transfer of a phosphate group from ATP to CMP, UMP, or dCMP, to form the corresponding diphosphate nucleotide. Alternate splicing results in both coding and non-coding transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

CMPK: Catalyzes specific phosphoryl transfer from ATP to UMP and CMP. Belongs to the adenylate kinase family.

Protein type: EC 2.7.4.14; Nucleotide Metabolism - pyrimidine; Kinase, other; EC 2.7.4.6

Chromosomal Location of Human Ortholog: 1p32

Cellular Component: nucleoplasm; cytoplasm; nucleolus; cytosol; nucleus

Molecular Function: uridine kinase activity; UMP kinase activity; nucleoside diphosphate kinase activity; cytidylate kinase activity; ATP binding

Biological Process: nucleobase, nucleoside and nucleotide metabolic process; nucleobase, nucleoside and nucleotide interconversion; UMP biosynthetic process; phthalate metabolic process; pyrimidine ribonucleotide biosynthetic process; nucleoside diphosphate phosphorylation; UDP biosynthetic process; ovulation cycle process; 'de novo' pyrimidine base biosynthetic process; CDP biosynthetic process; dUDP biosynthetic process; nucleoside triphosphate biosynthetic process; dCDP biosynthetic process