Rat CD169 Monoclonal Antibody | anti-CD169 antibody

RAT ANTI MOUSE CD169:FITC

Cat. Num. AAA216655

• Gene Names: Siglec1; Sn; Cd169; Siglec-1
• Applications: Flow Cytometry, Functional Assay
• Synonyms: CD169; Monoclonal Antibody; RAT ANTI MOUSE CD169:FITC; anti-CD169 antibody

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 3D6.112
• Form/Format: FITC; Purified IgG conjugated to Fluorescein Isothiocyanate Isomer 1 (FITC) - liquid
• Concentration: IgG concentration 0.1 mg/ml (varies by lot)
• Sequence Length: 1701

• Applicable Applications for anti-CD169 antibody: Flow cytometry (FC/FACS)
• Application Notes: Flow Cytometry: The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.; Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/5
• Perservative Stabilisers: 0.09% Sodium Azide; 1% Bovine Serum Albumin; Preparation
• Buffer Solution: Phosphate buffered saline
• Target Species: Mouse
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. This product should be stored undiluted. Storage in frost free freezers is not recommended. This product is photosensitive and should be protected from light. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data

(Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 (MBS216653), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

Testing Data

(Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 (MBS216653) followed by Goat anti Rat IgG antibody (MBS235195) as a detection reagent. Low power)

Testing Data

(Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 (MBS216653) followed by Goat anti Rat IgG antibody (MBS235195) as a detection reagent. High power)

Testing Data

(Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 (MBS216653) followed by Goat anti Rat IgG antibody (MBS235195) as a detection reagent. Medium power)

Testing Data

(Mouse peritoneal macrophages stained with Rat anti Mouse CD169:FITC (MBS216654))

Testing Data

(Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112 (MBS216653), green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

Testing Data

(Frozen mouse spleen stained with Rat anti Mouse CD169 (MBS216653))

Related Product Information for anti-CD169 antibody

Rat anti Mouse CD169 antibody, clone 3D6.112 recognizes mouse CD169 also known as sialoadhesin, Sheep erythrocyte receptor or Siglec-1. CD169 is a 1695 amino acid, ~180kDa single pass, type 1 transmembrane glycoprotein containing a single Ig-like V-type domain and sixteen Ig-like C2-type domains. CD169 is a macrophage restricted receptor, preferentially binding to alpha 2,3 linked sialic acid residues (Crocker et al. 1991) and is expressed on stromal macrophages in many tissues, particularly in lymph nodes, bone marrow and on marginal metallophilic macrophages in the spleen (Morris et al. 1991). CD169 has been implicated in a number of roles including cell-cell interactions with lymphocytes (van den Berg et al. 1992) and granulocytes (Crocker et al. 1995). CD169 expressing macrophages have also been suggested to play a role in host resistance to lymphoma metastasis (Umansky et al. 1996). In pigs CD169 has also been identified as a macrophage restricted receptor for porcine reproductive and respiratory syndrome virus (Delputte et al. 2007). CD169 expressing macrophages have also been implicated in the regulation of autoimmune disease progression through their interaction with regulatory T cells via CD169 (Wu et al. 2009). CD169 has also been shown to play a critical role in the recognition and elimination of invasive sialylated microorganisms including Campylobacter jejuni (Klass et al. 2012) and group B Streptococcus (Chang et al. 2014). The functional activity of rat anti mouse CD169 antibody, clone 3D6.112, its ability to inhibit binding of red blood cells to CD169 can be considerably enhanced by derivitization of the antibody with polyethylene glycol (Ducreux et al. 2008).

NCBI and Uniprot Product Information

• NCBI GI #: 226958332
• NCBI GeneID: 20612
• NCBI Accession #: NP_035556.3
• NCBI GenBank Nucleotide #: NM_011426.3
• UniProt Accession #: Q62230; O55216; Q62228; Q62229; D3YVZ3; D3YVZ4
• Molecular Weight: 172,769 Da
• NCBI Official Full Name: sialoadhesin
• NCBI Official Synonym Full Names: sialic acid binding Ig-like lectin 1, sialoadhesin
• NCBI Official Symbol: Siglec1
• NCBI Official Synonym Symbols: Sn; Cd169; Siglec-1
• NCBI Protein Information: sialoadhesin; SER; sheep erythrocyte receptor; sialic acid-binding Ig-like lectin 1
• UniProt Protein Name: Sialoadhesin
• UniProt Gene Name: Siglec1
• UniProt Synonym Gene Names: Sa; Sn; SER; Siglec-1
• UniProt Entry Name: SN_MOUSE

Uniprot Description

SIGLEC1: Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid. Binds to SPN/CD43 on T-cells. May play a role in hemopoiesis. Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral

Cellular Component: membrane; extracellular region; integral to membrane; plasma membrane

Molecular Function: protein binding; carbohydrate binding

Biological Process: endocytosis; cell adhesion