Mouse sonic hedgehog homolog (Drosophila) ELISA Kit | SHH elisa kit

Mouse Sonic hedgehog protein, SHH ELISA Kit

Cat. Num. AAA907964

• Gene Names: Shh; Hx; Dsh; Hhg1; Hxl3; M100081; 9530036O11Rik
• Reactivity: Mouse
• Synonyms: sonic hedgehog homolog (Drosophila); Mouse Sonic hedgehog protein; SHH ELISA Kit; Mouse Sonic hedgehog protein (SHH) ELISA kit; HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS; sonic hedgehog; SHH elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Mouse SHH. No significant cross-reactivity or interference between Mouse SHH and analogues was observed.
• Sequence Length: 437

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 28 pg/ml-1800 pg/ml
• Sensitivity: 7 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SHH elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SHH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SHH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SHH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SHH bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 21617861
• NCBI GeneID: 20423
• NCBI Accession #: NP_033196.1
• NCBI GenBank Nucleotide #: NM_009170.3
• UniProt Accession #: Q62226
• Molecular Weight: 47,773 Da
• NCBI Official Full Name: sonic hedgehog protein
• NCBI Official Synonym Full Names: sonic hedgehog
• NCBI Official Symbol: Shh
• NCBI Official Synonym Symbols: Hx; Dsh; Hhg1; Hxl3; M100081; 9530036O11Rik
• NCBI Protein Information: sonic hedgehog protein; HHG-1; short digits; hemimelic extra toes
• UniProt Protein Name: Sonic hedgehog protein
• Protein Family: Sonic hedgehog protein
• UniProt Gene Name: Shh
• UniProt Synonym Gene Names: Hhg1; SHH
• UniProt Entry Name: SHH_MOUSE

Uniprot Description

SHH: Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Interacts with HHATL/GUP1 which negatively regulates HHAT-mediated palmitoylation of the SHH N-terminus. N-product is active as a multimer. Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues. Belongs to the hedgehog family.

Protein type: Cell development/differentiation; Oncoprotein; Motility/polarity/chemotaxis; Cell cycle regulation

Cellular Component: Golgi apparatus; extracellular space; proteinaceous extracellular matrix; transport vesicle; cell surface; cell soma; membrane; axon; endoplasmic reticulum; dendrite; extracellular region; plasma membrane; nucleus; lipid raft

Molecular Function: peptidase activity; laminin-1 binding; protein binding; glycosaminoglycan binding; hydrolase activity; zinc ion binding; metal ion binding; patched binding; calcium ion binding; glycoprotein binding

Biological Process: prostate gland development; skin development; developmental growth; central nervous system development; embryonic skeletal development; positive regulation of transcription, DNA-dependent; mesenchymal cell proliferation; telencephalon regionalization; positive regulation of mesenchymal cell proliferation; embryonic foregut morphogenesis; male genitalia development; oligodendrocyte differentiation; anatomical structure formation; kidney development; inner ear development; neural crest cell migration; hindbrain development; embryonic limb morphogenesis; positive regulation of neuroblast proliferation; tongue morphogenesis; camera-type eye development; cell fate commitment; myotube differentiation; neuron fate commitment; intermediate filament organization; positive regulation of skeletal muscle cell proliferation; osteoblast development; response to ethanol; positive regulation of photoreceptor cell differentiation; regulation of gene expression; positive regulation of cell division; regulation of proteolysis; embryonic organ development; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; negative regulation of protein catabolic process; determination of left/right symmetry; neural tube formation; negative regulation of apoptosis; embryonic forelimb morphogenesis; limb development; axon guidance; granule cell precursor proliferation; spinal cord dorsal/ventral patterning; thalamus development; negative regulation of transcription from RNA polymerase II promoter; positive regulation of striated muscle cell differentiation; palate development; signal transduction; Bergmann glial cell differentiation; negative regulation of T cell proliferation; positive regulation of cell proliferation; forebrain development; pancreas development; thyroid gland development; embryonic morphogenesis; angiogenesis; heart looping; ventral spinal cord interneuron specification; vasculogenesis; negative regulation of cell migration; positive regulation of granule cell precursor proliferation; positive thymic T cell selection; gut mesoderm development; intein-mediated protein splicing; regulation of odontogenesis; androgen metabolic process; spinal cord motor neuron differentiation; pattern specification process; regulation of cell proliferation; odontogenesis of dentine-containing teeth; cell proliferation; embryonic development; stem cell development; negative regulation of cell differentiation; positive regulation of protein import into nucleus; dorsal/ventral pattern formation; ureteric bud branching; hindgut morphogenesis; positive regulation of neuron differentiation; negative regulation of alpha-beta T cell differentiation; lung development; negative regulation of Wnt receptor signaling pathway; anatomical structure development; heart development; multicellular organismal development; T cell differentiation in the thymus; CD4-positive or CD8-positive, alpha-beta T cell lineage commitment; lymphoid progenitor cell differentiation; Wnt receptor signaling pathway through beta-catenin; proteolysis; anterior/posterior pattern formation; positive regulation of T cell differentiation in the thymus; cell-cell signaling; hair follicle development; embryonic digestive tract morphogenesis; midbrain development; ectoderm development; positive regulation of smoothened signaling pathway; positive regulation of oligodendrocyte differentiation; oligodendrocyte development; activation of hh target transcription factor; striated muscle development; digestive tract morphogenesis; endocytosis; positive regulation of skeletal muscle development; negative thymic T cell selection; patterning of blood vessels; branching morphogenesis of a tube; polarity specification of anterior/posterior axis; positive regulation of cell differentiation; metanephros development; tongue development; apoptosis; cell fate specification; embryonic hindlimb morphogenesis; odontogenesis; vasculature development; regulation of transcription, DNA-dependent; male genitalia morphogenesis; cell communication; response to axon injury; positive regulation of Wnt receptor signaling pathway; dorsoventral neural tube patterning; smoothened signaling pathway; organ formation; hair follicle morphogenesis; thymus development; smoothened signaling pathway in regulation of granule cell precursor cell proliferation; respiratory tube development; positive regulation of immature T cell proliferation in the thymus; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; formation of anatomical boundary; myoblast differentiation; limb bud formation; establishment of cell polarity; neuroblast proliferation; blood coagulation; cell development; positive regulation of alpha-beta T cell differentiation