Sonic Hedgehog Active Protein | SHH active protein

Sonic Hedgehog Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8123127

• Gene Names: Shh; Hx; Dsh; Hhg1; Hxl3; M100081; 9530036O11Rik
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Sonic Hedgehog; Sonic Hedgehog Protein; Mouse; Recombinant (His Tag); Mouse SHH/Sonic hedgehog Protein (His Tag); 9530036O11Rik Protein; Dsh Protein; Hhg1 Protein; Hx Protein; Hxl3 Protein; M100081 Protein; sonic hedgehog; SHH active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Gly198

• Species: Mouse
• Activity: Measured by its ability to induce alkaline phosphatase production by C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 5-40ug/mL.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Cys 25
• Tag: C-His
• Protein Construction: A DNA sequence encoding the mouse SHH (Q62226)(Met1-Gly198) was expressed with a C-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

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Related Product Information for SHH active protein

Background: Sonic HedgeHog, also known as sonic hedgehog protein, belongs to the hedgehog family. It cannot be detected in adult tissues while can be found in fetal intestine, liver, lung, and kidney. Sonic HedgeHog is a protein that is vital in guiding the early embryo. It has been associated as the major inductive signal in patterning of the ventral neural tube, the anterior-posterior limb axis, and the ventral somites. Sonic HedgeHog intercellular signal is essential for a various patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Sonic HedgeHog binds to the patched receptor, which functions in association with smoothened, to activate the transcription of target genes. In the absence of sonic HedgeHog, patched receptor represses the constitutive signaling activity of smoothened. Sonic HedgeHog also regulates another factor, the gli oncogene. Defects in sonic hedgehog can cause microphthalmia isolated with coloboma type 5, triphalangeal thumb-polysyndactyly syndrome and holoprosencephaly type 3.

References

Ericson J, et al. (1997) Graded sonic hedgehog signaling and the specification of cell fate in the ventral neural tube. Cold Spring Harb Symp Quant Biol. 62:451-66.Marigo V, et al. (1996) Regulation of patched by sonic hedgehog in the developing neural tube. Proc Natl Acad Sci. 93(18):9346-51.Stone DM, et al. (1996) he tumour-suppressor gene patched encodes a candidate receptor for Sonic hedgehog. Nature. 384:129-34. Nakamura, T. et al. (1997) Biochem. Biophys. Res. Commun.237:465.

NCBI and Uniprot Product Information

• NCBI GI #: 21617861
• NCBI GeneID: 20423
• NCBI Accession #: NP_033196.1
• NCBI GenBank Nucleotide #: NM_009170.3
• UniProt Accession #: Q62226
• Molecular Weight: 47,773 Da
• NCBI Official Full Name: sonic hedgehog protein
• NCBI Official Synonym Full Names: sonic hedgehog
• NCBI Official Symbol: Shh
• NCBI Official Synonym Symbols: Hx; Dsh; Hhg1; Hxl3; M100081; 9530036O11Rik
• NCBI Protein Information: sonic hedgehog protein; HHG-1; hemimelic extra toes; short digits
• UniProt Protein Name: Sonic hedgehog protein
• Protein Family: Sonic hedgehog protein
• UniProt Gene Name: Shh
• UniProt Synonym Gene Names: Hhg1; SHH
• UniProt Entry Name: SHH_MOUSE

Uniprot Description

SHH: Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. Interacts with HHATL/GUP1 which negatively regulates HHAT-mediated palmitoylation of the SHH N-terminus. N-product is active as a multimer. Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues. Belongs to the hedgehog family.

Protein type: Cell development/differentiation; Oncoprotein; Cell cycle regulation; Motility/polarity/chemotaxis

Cellular Component: Golgi apparatus; extracellular space; cell surface; endoplasmic reticulum; dendrite; extracellular region; lipid raft; proteinaceous extracellular matrix; transport vesicle; membrane; cell soma; axon; plasma membrane; nucleus

Molecular Function: laminin-1 binding; peptidase activity; protein binding; glycosaminoglycan binding; zinc ion binding; hydrolase activity; metal ion binding; patched binding; calcium ion binding; glycoprotein binding

Biological Process: prostate gland development; skin development; developmental growth; central nervous system development; positive regulation of transcription, DNA-dependent; embryonic skeletal development; mesenchymal cell proliferation; telencephalon regionalization; embryonic foregut morphogenesis; positive regulation of mesenchymal cell proliferation; male genitalia development; anatomical structure formation; oligodendrocyte differentiation; kidney development; neural crest cell migration; inner ear development; embryonic limb morphogenesis; hindbrain development; positive regulation of neuroblast proliferation; tongue morphogenesis; camera-type eye development; cell fate commitment; myotube differentiation; neuron fate commitment; intermediate filament organization; osteoblast development; positive regulation of skeletal muscle cell proliferation; response to ethanol; positive regulation of photoreceptor cell differentiation; regulation of gene expression; positive regulation of cell division; regulation of proteolysis; striated muscle cell differentiation; embryonic organ development; positive regulation of transcription from RNA polymerase II promoter; embryonic digit morphogenesis; neural tube formation; determination of left/right symmetry; negative regulation of protein catabolic process; negative regulation of apoptosis; embryonic forelimb morphogenesis; limb development; axon guidance; granule cell precursor proliferation; spinal cord dorsal/ventral patterning; positive regulation of striated muscle cell differentiation; negative regulation of transcription from RNA polymerase II promoter; thalamus development; palate development; signal transduction; Bergmann glial cell differentiation; negative regulation of T cell proliferation; positive regulation of cell proliferation; forebrain development; pancreas development; thyroid gland development; embryonic morphogenesis; heart looping; angiogenesis; vasculogenesis; ventral spinal cord interneuron specification; negative regulation of cell migration; positive thymic T cell selection; positive regulation of granule cell precursor proliferation; gut mesoderm development; intein-mediated protein splicing; regulation of odontogenesis; androgen metabolic process; spinal cord motor neuron differentiation; pattern specification process; odontogenesis of dentine-containing teeth; regulation of cell proliferation; cell proliferation; negative regulation of cell differentiation; stem cell development; embryonic development; dorsal/ventral pattern formation; positive regulation of protein import into nucleus; ureteric bud branching; hindgut morphogenesis; positive regulation of neuron differentiation; lung development; negative regulation of alpha-beta T cell differentiation; negative regulation of Wnt receptor signaling pathway; anatomical structure development; multicellular organismal development; heart development; CD4-positive or CD8-positive, alpha-beta T cell lineage commitment; T cell differentiation in the thymus; lymphoid progenitor cell differentiation; Wnt receptor signaling pathway through beta-catenin; proteolysis; anterior/posterior pattern formation; positive regulation of T cell differentiation in the thymus; cell-cell signaling; hair follicle development; embryonic digestive tract morphogenesis; midbrain development; ectoderm development; positive regulation of smoothened signaling pathway; positive regulation of oligodendrocyte differentiation; oligodendrocyte development; activation of hh target transcription factor; striated muscle development; endocytosis; digestive tract morphogenesis; positive regulation of skeletal muscle development; negative thymic T cell selection; patterning of blood vessels; branching morphogenesis of a tube; polarity specification of anterior/posterior axis; positive regulation of cell differentiation; metanephros development; tongue development; apoptosis; cell fate specification; embryonic hindlimb morphogenesis; odontogenesis; vasculature development; regulation of transcription, DNA-dependent; male genitalia morphogenesis; cell communication; response to axon injury; positive regulation of Wnt receptor signaling pathway; dorsoventral neural tube patterning; organ formation; smoothened signaling pathway; hair follicle morphogenesis; thymus development; smoothened signaling pathway in regulation of granule cell precursor cell proliferation; respiratory tube development; positive regulation of immature T cell proliferation in the thymus; negative regulation of proteasomal ubiquitin-dependent protein catabolic process; formation of anatomical boundary; myoblast differentiation; limb bud formation; establishment of cell polarity; neuroblast proliferation; blood coagulation; cell development; positive regulation of alpha-beta T cell differentiation