Rat surfactant protein D ELISA Kit | SFTPD elisa kit

Rat Pulmonary Surfactant-associated protein D, SP-D ELISA Kit

Cat. Num. AAA703468

• Gene Names: Sftpd; SPD; SP-D
• Reactivity: Rat
• Synonyms: surfactant protein D; Rat Pulmonary Surfactant-associated protein D; SP-D ELISA Kit; Rat Pulmonary Surfactant-associated protein D (SP-D) ELISA kit; COLEC7; PSP-D; SFTP4; SP-D; collectin 7; pulmonary surfactant apoprotein; pulmonary surfactant-associated protein D; surfactant; pulmonary-associated protein D; surfactant-associated protein; pulmonary; SFTPD elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of rat SP-D. No significant cross-reactivity or interference between rat SP-D and analogues was observed.
• Sequence Length: 374

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 0.312 ng/ml -20 ng/mL
• Sensitivity: <0.078 ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SFTPD elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP-D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP-D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP-D is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP-D bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 7949133
• NCBI GeneID: 25350
• NCBI Accession #: NP_037010.1
• NCBI GenBank Nucleotide #: NM_012878.2
• UniProt Accession #: P35248
• Molecular Weight: 37,561 Da
• NCBI Official Full Name: pulmonary surfactant-associated protein D
• NCBI Official Synonym Full Names: surfactant protein D
• NCBI Official Symbol: Sftpd
• NCBI Official Synonym Symbols: SPD; SP-D
• NCBI Protein Information: pulmonary surfactant-associated protein D; CP4; PSP-D; lung surfactant protein D; pulmonary surfactant protein D; surfactant associated protein D; surfactant, pulmonary-associated protein D
• UniProt Protein Name: Pulmonary surfactant-associated protein D
• Protein Family: Pulmonary surfactant-associated protein
• UniProt Gene Name: Sftpd
• UniProt Synonym Gene Names: Sftp4; PSP-D; SP-D
• UniProt Entry Name: SFTPD_RAT

NCBI Description

binds carbohydrates; may play a role in defense response in the lung [RGD, Feb 2006]

Uniprot Description

Function: Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.