Rat Pulmonary Surfactant-Associated Protein D (SP-D) ELISA Kit | SP-D elisa kit

Rat Pulmonary Surfactant-Associated Protein D (SP-D) ELISA Kit

Cat. Num. AAA9711885

• Gene Names: Sftpd; SPD; SP-D
• Reactivity: Rat
• Synonyms: Pulmonary Surfactant-Associated Protein D (SP-D); Rat Pulmonary Surfactant-Associated Protein D (SP-D) ELISA Kit; SFTPD; COLEC7; PSP-D; SFTP4; SP-D; collectin 7; pulmonary surfactant apoprotein; pulmonary surfactant-associated protein D; surfactant; pulmonary-associated protein D; surfactant-associated protein; pulmonary; SP-D elisa kit

• Reactivity: Rat
• Specificity: Rat Pulmonary Surfactant-associated protein D (SP-D) ELISA Kit has high sensitivity and excellent specificity for detection of Rat SFTPD. No significant cross-reactivity or interference between Rat SFTPD and analogues was observed.
• Sequence Length: 374

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Rat Pulmonary Surfactant-associated protein D (SP-D) Standard Curve.)

Related Product Information for SP-D elisa kit

Description: This Rat Pulmonary Surfactant-associated protein D (SP-D) ELISA Kit employs a two-site sandwich ELISA to quantitate SFTPD in samples. An antibody specific for SFTPD has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFTPD present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFTPD is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFTPD bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: Surfactant protein D (SP-D) belongs to the collectin family.These proteins are oligomeric proteins composed of carbohydrate-recognition domains(CRD)attached to collagenous regions.Collectins are structurally similar to the ficolins although they make use of different CRD structures:C-type lectin domain for the collectins.The lung is the major site of synthesis of SP-D, where the molecules are produced and secreted onto the epithelial surface by alveolar type IIcells and unciliated bronchial epithelial cells. SP-D is also found in different epithelial cells of the gastrointestinal tract and in epithelial cells of various exocrine glands. SP-D is an important factor in the pulmonary anti-microbial defense. The anti-microbial defense mechanisms of SP-D are direct opsonization, neutralization and agglutination.

NCBI and Uniprot Product Information

• NCBI GI #: 7949133
• NCBI GeneID: 25350
• NCBI Accession #: NP_037010.1
• NCBI GenBank Nucleotide #: NP_037010.1
• UniProt Accession #: P35248
• NCBI Official Full Name: pulmonary surfactant-associated protein D
• NCBI Official Synonym Full Names: surfactant protein D
• NCBI Official Symbol: Sftpd
• NCBI Official Synonym Symbols: SPD; SP-D
• NCBI Protein Information: pulmonary surfactant-associated protein D
• UniProt Protein Name: Pulmonary surfactant-associated protein D
• UniProt Gene Name: Sftpd
• UniProt Synonym Gene Names: Sftp4; PSP-D; SP-D

NCBI Description

binds carbohydrates; may play a role in defense response in the lung [RGD, Feb 2006]

Uniprot Description

SFTPD: Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. Belongs to the SFTPD family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16p14

Cellular Component: collagen; cytoplasm; cytoplasmic vesicle; extracellular space; multivesicular body; proteinaceous extracellular matrix; rough endoplasmic reticulum

Molecular Function: identical protein binding; lipopolysaccharide binding; monosaccharide binding

Biological Process: alveolus development; induction of bacterial agglutination; innate immune response; negative regulation by host of symbiont molecular function; negative regulation of interleukin-2 production; negative regulation of phagocytosis; negative regulation of T cell proliferation; opsonization; positive regulation of phagocytosis; regulation of liquid surface tension; respiratory gaseous exchange; response to corticosteroid stimulus; response to glucocorticoid stimulus; response to hyperoxia; surfactant homeostasis