Mouse SP-D ELISA Kit | SFTPD elisa kit

Mouse SP-D PicoKine ELISA Kit

Cat. Num. AAA177306

• Gene Names: Sftpd; SP-D; Sftp4; AI573415
• Reactivity: Mouse
• Synonyms: SP-D; Mouse SP-D PicoKine ELISA Kit; Pulmonary surfactant-associated protein D; surfactant protein D; COLEC 7; COLEC7; Collectin-7; Collectin7; Lung surfactant protein D; PSP D; PSP-D; PSPD; Pulmonary surfactant apoprotein; Pulmonary surfactant associated protein D; SFTP 4; SFTP4; SFTPD; SFTPD_HUMAN; SP D; Surfactant associated protein pulmonary 4; Surfactant pulmonary associated protein D; SFTPD elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse SP-D
• Sequence Length: 374

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 312pg/ml-20,000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse SP-D PicoKine ELISA Kit standard curve )

Related Product Information for SFTPD elisa kit

Principle of the assay: mouse SP-D ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for SP-D has been precoated onto 96-well plates. Standards(CHO, A20-F374) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SP-D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse SP-D amount of sample captured in plate.
Background: Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What's more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.

NCBI and Uniprot Product Information

• NCBI GI #: 1709879
• NCBI GeneID: 20390
• UniProt Accession #: P50404
• Molecular Weight: 37,688 Da
• NCBI Official Full Name: Pulmonary surfactant-associated protein D
• NCBI Official Synonym Full Names: surfactant associated protein D
• NCBI Official Symbol: Sftpd
• NCBI Official Synonym Symbols: SP-D; Sftp4; AI573415
• NCBI Protein Information: pulmonary surfactant-associated protein D; PSP-D; lung surfactant protein D
• UniProt Protein Name: Pulmonary surfactant-associated protein D
• Protein Family: Pulmonary surfactant-associated protein
• UniProt Gene Name: Sftpd
• UniProt Synonym Gene Names: Sftp4; PSP-D; SP-D
• UniProt Entry Name: SFTPD_MOUSE

Uniprot Description

SFTPD: Contributes to the lung's defense against inhaled microorganisms. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. Belongs to the SFTPD family.

Protein type: Secreted; Secreted, signal peptide

Cellular Component: multivesicular body; proteinaceous extracellular matrix; extracellular space; collagen; rough endoplasmic reticulum; cytoplasm; extracellular region; cytoplasmic vesicle

Molecular Function: monosaccharide binding; identical protein binding; lipopolysaccharide binding; carbohydrate binding

Biological Process: negative regulation of phagocytosis; negative regulation of T cell proliferation; immune system process; positive regulation of phagocytosis; innate immune response; opsonization; respiratory gaseous exchange; regulation of liquid surface tension; surfactant homeostasis; alveolus development; negative regulation of interleukin-2 production