Mouse pulmonary surfactant-associated protein D (SP-D) ELISA Kit | SP-D elisa kit

Mouse pulmonary surfactant-associated protein D, SP-D ELISA Kit

Cat. Num. AAA703398

• Gene Names: SFTPD; SP-D; PSP-D; SFTP4; COLEC7
• Reactivity: Mouse
• Synonyms: pulmonary surfactant-associated protein D (SP-D); Mouse pulmonary surfactant-associated protein D; SP-D ELISA Kit; Mouse pulmonary surfactant-associated protein D (SP-D) ELISA kit; SP-D elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse SP-D. No significant cross-reactivity or interference between mouse SP-D and analogues was observed.
• Sequence Length: 375

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 1.56 ng/ml-100 ng/ml
• Sensitivity: < 0.39 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SP-D elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SP-D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SP-D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SP-D is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SP-D bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 292507
• NCBI GeneID: 6441
• UniProt Accession #: P35247; Q5T0M3; Q6FH08; Q86YK9; Q8TCD8; Q9UCJ2; Q9UCJ3
• Molecular Weight: 37,728 Da
• NCBI Official Full Name: surfactant protein D
• NCBI Official Synonym Full Names: surfactant protein D
• NCBI Official Symbol: SFTPD
• NCBI Official Synonym Symbols: SP-D; PSP-D; SFTP4; COLEC7
• NCBI Protein Information: pulmonary surfactant-associated protein D; collectin 7; collectin-7; lung surfactant protein D; pulmonary surfactant apoprotein; surfactant, pulmonary-associated protein D; surfactant-associated protein, pulmonary 4
• UniProt Protein Name: Pulmonary surfactant-associated protein D
• UniProt Gene Name: SFTPD
• UniProt Synonym Gene Names: COLEC7; PSPD; SFTP4; PSP-D; SP-D
• UniProt Entry Name: SFTPD_HUMAN

NCBI Description

The protein encoded by this gene is part of the innate immune response, protecting the lungs against inhaled microorganisms and chemicals. The encoded protein may also be involved in surfactant metabolism. [provided by RefSeq, Jul 2015]

Uniprot Description

Function: Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties.1 PublicationManual assertion based on experiment in:Ref.9