sFRP4 recombinant protein

Recombinant Mouse sFRP4 Protein (His tag)

Cat. Num. AAA2547179

• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: sFRP4; Recombinant Mouse sFRP4 Protein (His tag); Sfrp4; sFRP4 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 351

• Application Notes: The recombinant mouse sFRP4 consists of 341 amino acids after removal of the signal peptide and has a predicted molecular mass of 39.4 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 55-60 kDa due to glycosylation.
• Predicted N Terminal: Ala 22
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for sFRP4 recombinant protein

Background: SFRP family consists of five secreted glycoproteins in humans acting as extracellular signaling ligands. Each is approximately 300 amino acids in length and contains a cysteine-rich domain (CRD) that shares 30-50% sequence homology with the CRD of Frizzled  (Fz) receptors, a putative signal sequence, and a conserved hydrophilic carboxy-terminal domain. SFRPs act as soluble modulators of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations. SFRPs are able to bind Wnt proteins and Fz receptors in the extracellular compartment. The interaction between SFRPs and Wnt proteins prevents the latter from binding the Fz receptors. The Wnt pathway plays a key role in embryonic development, cell differentiation and cell proliferation. SFRP4 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins called FZ domain and a NTR domain. Mouse SFRP4 is highly expressed in the ovary, and is localized to granulosa cells of periovulatory follicles and corpora lutea. It plays a critical role in placental development and implantation, and is also an important factor in the development of the decidual fibrinoid zone, and in trophoblast apoptosis.

Description: A DNA sequence encoding the mouse sFRP4 (NP_057896.1) (Met 1-Ser 351) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 7710094
• NCBI GeneID: 20379
• NCBI Accession #: NP_057896.1
• NCBI GenBank Nucleotide #: NM_016687.3
• UniProt Accession #: Q9Z1N6; Q91ZX9
• Molecular Weight: 40,342 Da
• NCBI Official Full Name: secreted frizzled-related sequence protein 4
• NCBI Official Synonym Full Names: secreted frizzled-related protein 4
• NCBI Official Symbol: Sfrp4
• NCBI Protein Information: secreted frizzled-related sequence protein 4
• UniProt Protein Name: Secreted frizzled-related sequence protein 4
• Protein Family: Secreted frizzled-related protein
• UniProt Gene Name: Sfrp4
• UniProt Synonym Gene Names: FRP-4; sFRP-4

Uniprot Description

SFRP4: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13 A2|13 6.99 cM

Cellular Component: cell surface; cytoplasm; extracellular space; integral to membrane; nucleus

Molecular Function: G-protein coupled receptor activity; Wnt receptor activity; Wnt-protein binding

Biological Process: negative regulation of cell proliferation; negative regulation of JNK cascade; negative regulation of transcription factor activity; phosphate ion homeostasis; positive regulation of apoptosis; positive regulation of epidermal cell differentiation; positive regulation of receptor internalization; Wnt receptor signaling pathway through beta-catenin