sFRP2 recombinant protein

Recombinant Mouse sFRP2 Protein (His tag)

Cat. Num. AAA2547201

• Gene Names: Sfrp2; Sdf5; AI851596
• Purity: > 95 % as determined by SDS-PAGE
• Synonyms: sFRP2; Recombinant Mouse sFRP2 Protein (His tag); Sfrp2; AI851596; Sdf5; sFRP2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 95 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 295

• Application Notes: The recombinant mouse sFRP2 consists of 282 amino acids after removal of the signal peptide and has a predicted molecular mass of 32.5 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 36 kDa due to glycosylation.
• Predicted N Terminal: Leu 25
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for sFRP2 recombinant protein

Background: The Secreted frizzled-related protein (SFRP) family consists of five secreted glycoproteins in humans (SFRP1~5) that act as extracellular signaling ligands. Each SFRP is approximately 300 amino acids in length and contains a cysteine-rich domain (CRD) that shares 30-50% sequence homology with the CRD of Frizzled  (Fz) receptors, a putative signal sequence, and a conserved hydrophilic carboxy-terminal domain. SFRPs are able to bind Wnt proteins and Fz receptors in the extracellular compartment. The interaction between SFRPs and Wnt proteins prevents the latter from binding the Fz receptors. The Wnt pathway plays a key role in embryonic development, cell differentiation and cell proliferation. sFRP2 is a member of the SFRP family acting as soluble modulators of Wnt signaling and contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins called FZ domain and a NTR domain.sFRP2 inhibites hypoxia induced endothelial cell apoptosis and increases endothelial cell migration. It prevents mesoderm specification and maintains the cells in the undifferentiated state. SFRP2 is also a novel stimulator of angiogenesis that stimulates angiogenesis via a calcineurin/NFAT pathway, thus is regarded as a favorable target for the inhibition of angiogenesis in solid tumors. Mouse sFRP2 is highly expressed in the eye and is also detected in heart and lung at low level.

Description: A DNA sequence encoding the mouse sFRP2 (NP_033170.1) (Met 1-Cys 295) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 6677895
• NCBI GeneID: 20319
• NCBI Accession #: NP_033170.1
• NCBI GenBank Nucleotide #: NM_009144.2
• UniProt Accession #: P97299; O08862; O35297
• Molecular Weight: 33,469 Da
• NCBI Official Full Name: secreted frizzled-related protein 2
• NCBI Official Synonym Full Names: secreted frizzled-related protein 2
• NCBI Official Symbol: Sfrp2
• NCBI Official Synonym Symbols: Sdf5; AI851596
• NCBI Protein Information: secreted frizzled-related protein 2
• UniProt Protein Name: Secreted frizzled-related protein 2
• Protein Family: Secreted frizzled-related protein
• UniProt Gene Name: Sfrp2
• UniProt Synonym Gene Names: Sarp1; Sdf5; sFRP-2; SARP-1

Uniprot Description

SFRP2: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP2 may be important for eye retinal development and for myogenesis. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3 E3|3 37.37 cM

Cellular Component: extracellular space; integral to membrane

Molecular Function: enzyme activator activity; G-protein coupled receptor activity; protein binding; receptor agonist activity; Wnt receptor activity; Wnt-protein binding

Biological Process: anterior/posterior pattern formation; apoptosis; cartilage development; cellular response to extracellular stimulus; chondrocyte development; collagen fibril organization; convergent extension involved in axis elongation; development of primary male sexual characteristics; digestive tract morphogenesis; embryonic digit morphogenesis; male gonad development; negative regulation of BMP signaling pathway; negative regulation of caspase activity; negative regulation of cell growth; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of JNK activity; negative regulation of mesodermal cell fate specification; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of transcription, DNA-dependent; negative regulation of Wnt receptor signaling pathway; neural tube closure; neural tube development; patterning of blood vessels; positive regulation of angiogenesis; positive regulation of apoptosis; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of fat cell differentiation; positive regulation of osteoblast differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of transcription from RNA polymerase II promoter; regulation of apoptosis; regulation of cell proliferation; regulation of Wnt receptor signaling pathway; response to drug; somitogenesis