Mouse secreted frizzled-related protein 4 ELISA Kit | SFRP4 elisa kit

Mouse Secreted frizzled-related protein 4, SFRP4 ELISA Kit

Cat. Num. AAA907810

• Reactivity: Mouse
• Synonyms: secreted frizzled-related protein 4; Mouse Secreted frizzled-related protein 4; SFRP4 ELISA Kit; Mouse Secreted frizzled-related protein 4 (SFRP4) ELISA kit; FRP-4; FRPHE; MGC26498; SFRP4 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of mouse SFRP4. No significant cross-reactivity or interference between mouse SFRP4 and analogues was observed.
• Sequence Length: 351

• Samples: Serum, plasma, cell culture supernates, tissue homogenates
• Detection Range: 0.47 ng/ml -30 ng/mL
• Sensitivity: <0.11 ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SFRP4 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SFRP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFRP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFRP4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFRP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 7710094
• NCBI GeneID: 20379
• NCBI Accession #: NP_057896.1
• NCBI GenBank Nucleotide #: NM_016687.3
• UniProt Accession #: Q9Z1N6; Q91ZX9
• Molecular Weight: 40,342 Da
• NCBI Official Full Name: secreted frizzled-related sequence protein 4
• NCBI Official Synonym Full Names: secreted frizzled-related protein 4
• NCBI Official Symbol: Sfrp4
• NCBI Protein Information: secreted frizzled-related sequence protein 4; FRP-4; sFRP-4; frizzled-related protein 4
• UniProt Protein Name: Secreted frizzled-related sequence protein 4
• Protein Family: Secreted frizzled-related protein
• UniProt Gene Name: Sfrp4
• UniProt Synonym Gene Names: FRP-4; sFRP-4
• UniProt Entry Name: SFRP4_MOUSE

Uniprot Description

SFRP4: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; cell surface; cytoplasm; extracellular region; nucleus

Molecular Function: Wnt-protein binding

Biological Process: negative regulation of JNK cascade; negative regulation of cell proliferation; Wnt receptor signaling pathway; positive regulation of apoptosis; negative regulation of transcription factor activity; multicellular organismal development; positive regulation of epidermal cell differentiation; positive regulation of receptor internalization; phosphate ion homeostasis; cell differentiation