SFRP4 active protein

SFRP4 Protein, Human, Recombinant (His Tag)

Cat. Num. AAA8120452

• Gene Names: SFRP4; FRP-4; FRPHE; sFRP-4
• Purity: >95% as determined by SDS-PAGE
• Synonyms: SFRP4; SFRP4 Protein; Human; Recombinant (His Tag); Human sFRP4 Protein (His Tag); FRP-4 Protein; FRPHE Protein; sFRP-4 Protein; secreted frizzled-related protein 4; SFRP4 active protein

• Host: CHO Stable Cells
• Purity/Purification: >95% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-VAL346

• Species: Human
• Activity: Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells. The ED50 for this effect is typically 2-20ug/mL in the presence of 20ng/mL of Wnt3a.
• Endotoxin: <1.0EU per ug protein as determined by the LAL method.
• Predicted N Terminal: Ala 22
• Tag: C-His
• Protein Construction: A DNA sequence encoding the human SFRP4 (NP_003005.2)(Met1-VAL346) was expressed with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Testing Data

(Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells. The ED50 for this effect is typically 2-20 ug/mL in the presence of 20 ng/mL of Wnt3a.)

Related Product Information for SFRP4 active protein

Background: SFRP family consists of five secreted glycoproteins in humans acting as extracellular signaling ligands. Each is approximately 3 amino acids in length and contains a cysteine-rich domain (CRD) that shares 3-5% sequence homology with the CRD of Frizzled (Fz) receptors, a putative signal sequence, and a conserved hydrophilic carboxy-terminal domain. SFRPs act as soluble modulators of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations. SFRPs are able to bind Wnt proteins and Fz receptors in the extracellular compartment. The interaction between SFRPs and Wnt proteins prevents the latter from binding the Fz receptors. The Wnt pathway plays a key role in embryonic development, cell differentiation and cell proliferation. SFRP4 is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins called FZ domain and a NTR domain. Mouse SFRP4 is highly expressed in the ovary, and is localized to granulosa cells of periovulatory follicles and corpora lutea. It plays a critical role in placental development and implantation, and is also an important factor in the development of the decidual fibrinoid zone, and in trophoblast apoptosis.

References

Abu-Jawdeh G.M., et al.,(1999), Differential expression of frpHE: a novel human stromal protein of the secreted frizzled gene family, during the endometrial cycle and malignancy. Lab. Invest. 79:439-447.Berndt T., et al., (2003), Secreted frizzled-related protein 4 is a potent tumor-derived phosphaturic agent.J. Clin. Invest. 112:785-794.Ota T., et al.,(2004), Complete sequencing and characterization of 21,243 full-length human cDNAs.Nat. Genet. 36:40-45.

NCBI and Uniprot Product Information

• NCBI GI #: 170784838
• NCBI GeneID: 6424
• NCBI Accession #: NP_003005.2
• NCBI GenBank Nucleotide #: NM_003014.3
• UniProt Accession #: Q6FHJ7; O14877; Q05BG7; Q1ZYW2; Q4G124; Q6FHM0; Q6PD64; B4DYC1
• Molecular Weight: 39,827 Da
• NCBI Official Full Name: secreted frizzled-related protein 4
• NCBI Official Synonym Full Names: secreted frizzled-related protein 4
• NCBI Official Symbol: SFRP4
• NCBI Official Synonym Symbols: FRP-4; FRPHE; sFRP-4
• NCBI Protein Information: secreted frizzled-related protein 4; frizzled protein, human endometrium; secreted frizzled-related protein 4; secreted frizzled-related protein 4
• UniProt Protein Name: Secreted frizzled-related protein 4
• Protein Family: Secreted frizzled-related protein
• UniProt Gene Name: SFRP4
• UniProt Synonym Gene Names: FRPHE; sFRP-4; FrpHE
• UniProt Entry Name: SFRP4_HUMAN

NCBI Description

Secreted frizzled-related protein 4 (SFRP4) is a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. SFRPs act as soluble modulators of Wnt signaling. The expression of SFRP4 in ventricular myocardium correlates with apoptosis related gene expression. [provided by RefSeq, Jul 2008]

Uniprot Description

SFRP4: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP4 may act as a regulator of adult uterine morphology and function. Increases apoptosis during ovulation possibly through modulation of FZ1/FZ4/WNT4 signaling. Has phosphaturic effects by specifically inhibiting sodium-dependent phosphate uptake. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 7p14.1

Cellular Component: extracellular space; cell surface; cytoplasm; integral to membrane; nucleus

Molecular Function: G-protein coupled receptor activity; Wnt-protein binding; Wnt receptor activity; protein binding

Biological Process: negative regulation of cell proliferation; positive regulation of apoptosis; multicellular organismal development; negative regulation of transcription factor activity; positive regulation of epidermal cell differentiation; positive regulation of receptor internalization; response to hormone stimulus; phosphate ion homeostasis; cell differentiation