sFRP1 / SARP2 recombinant protein

Recombinant Human sFRP1 / SARP2 Protein (His tag)

Cat. Num. AAA2546146

• Gene Names: SFRP1; FRP; FRP1; FrzA; FRP-1; SARP2
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: sFRP1 / SARP2; Recombinant Human sFRP1 / SARP2 Protein (His tag); SFRP1; FRP; FRP-1; FRP1; FrzA; SARP2; sFRP1 / SARP2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 314

• Application Notes: The recombinant human sFRP1 consists of 294 amino acids after removal of the signal peptide and has a predicted molecular mass of 34 kDa. In SDS-PAGE under reducing conditions, it migrates with an apparent molecular mass of 38 kDa due to glycosylation.
• Predicted N Terminal: Ser 32
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for sFRP1 / SARP2 recombinant protein

Background: Secreted frizzled-related protein 1, also known as sFRP1, is a 35 kDa prototypical member of the SFRP family. SFRP family consists of five secreted glycoproteins in humans acting as extracellular signaling ligands. Each is approximately 300 amino acids in length and contains a cysteine-rich domain (CRD) that shares 30-50% sequence homology with the CRD of Frizzled  (Fz) receptors, a putative signal sequence, and a conserved hydrophilic carboxy-terminal domain. SFRPs act as soluble modulators of Wnt signaling, counteracting Wnt-induced effects at high concentrations and promoting them at lower concentrations. SFRPs are able to bind Wnt proteins and Fz receptors in the extracellular compartment. The interaction between SFRPs and Wnt proteins prevents the latter from binding the Fz receptors. The Wnt pathway plays a key role in embryonic development, cell differentiation and cell proliferation. The deregulation of this critical developmental pathway occurs in several human tumor entities. Mouse sFRP1 is highly expressed in kidney and embryonic heart, as well as in the eye, where it is principally localized to the ciliary body and the lens epithelium.

Description: A DNA sequence encoding the human sFRP1 (NP_003003.3) (Met 1-Lys 314) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 56117838
• NCBI GeneID: 6422
• NCBI Accession #: NP_003003.3
• NCBI GenBank Nucleotide #: NM_003012.4
• UniProt Accession #: Q8N474; O00546; O14779
• Molecular Weight: 35,386 Da
• NCBI Official Full Name: secreted frizzled-related protein 1
• NCBI Official Synonym Full Names: secreted frizzled related protein 1
• NCBI Official Symbol: SFRP1
• NCBI Official Synonym Symbols: FRP; FRP1; FrzA; FRP-1; SARP2
• NCBI Protein Information: secreted frizzled-related protein 1
• UniProt Protein Name: Secreted frizzled-related protein 1
• UniProt Gene Name: SFRP1
• UniProt Synonym Gene Names: FRP; FRP1; SARP2; FRP-1; sFRP-1; SARP-2

NCBI Description

This gene encodes a member of the SFRP family that contains a cysteine-rich domain homologous to the putative Wnt-binding site of Frizzled proteins. Members of this family act as soluble modulators of Wnt signaling; epigenetic silencing of SFRP genes leads to deregulated activation of the Wnt-pathway which is associated with cancer. This gene may also be involved in determining the polarity of photoreceptor cells in the retina. [provided by RefSeq, Sep 2009]

Uniprot Description

SFRP1: Soluble frizzled-related proteins (sFRPS) function as modulators of Wnt signaling through direct interaction with Wnts. They have a role in regulating cell growth and differentiation in specific cell types. SFRP1 decreases intracellular beta-catenin levels. Has antiproliferative effects on vascular cells, in vitro and in vivo, and can induce, in vivo, an angiogenic response. In vascular cell cycle, delays the G1 phase and entry into the S phase. In kidney development, inhibits tubule formation and bud growth in metanephroi. Inhibits WNT1/WNT4-mediated TCF-dependent transcription. Interacts with WNT1, WNT2 and FRZD6. Interacts with WNT4 and WNT8. Down-regulated in colorectal and breast tumors. Up- regulated in uterine leiomyomas under high estrogenic conditions. Expression, in leiomyomal cells, also increased both under hypoxic and serum deprivation conditions. Widely expressed. Absent from lung, liver and peripheral blood leukocytes. Highest levels in heart and fetal kidney. Also expressed in testis, ovary, fetal brain and lung, leiomyomal cells, myometrial cells and vascular smooth muscle cells. Expressed in foreskin fibroblasts and in keratinocytes. Belongs to the secreted frizzled-related protein (sFRP) family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p11.21

Cellular Component: cell surface; cytosol; extracellular matrix; extracellular region; extracellular space; integral to membrane; intracellular; plasma membrane; proteinaceous extracellular matrix

Molecular Function: cysteine-type endopeptidase activity; drug binding; frizzled binding; G-protein coupled receptor activity; heparin binding; identical protein binding; protein binding; Wnt receptor activity; Wnt-protein binding

Biological Process: cellular response to starvation; dorsal/ventral axis specification; hemopoietic progenitor cell differentiation; menstrual cycle phase; negative regulation of apoptosis; negative regulation of B cell differentiation; negative regulation of bone remodeling; negative regulation of cell growth; negative regulation of cell migration; negative regulation of cell proliferation; negative regulation of epithelial cell proliferation; negative regulation of fibroblast proliferation; negative regulation of insulin secretion; negative regulation of ossification; negative regulation of osteoblast proliferation; negative regulation of peptidyl-tyrosine phosphorylation; negative regulation of transcription, DNA-dependent; negative regulation of Wnt receptor signaling pathway; osteoblast differentiation; positive regulation of apoptosis; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of cell-matrix adhesion; positive regulation of epithelial cell proliferation; positive regulation of fat cell differentiation; positive regulation of focal adhesion formation; positive regulation of GTPase activity; positive regulation of smoothened signaling pathway; positive regulation of stress fiber formation; positive regulation of transcription, DNA-dependent; positive regulation of Wnt receptor signaling pathway; regulation of angiogenesis; response to drug; response to organic cyclic substance; Wnt receptor signaling pathway through beta-catenin