MSK2, active Active Protein | Mapk14 active protein

MSK2, active, Recombinant, Human (Mitogen and Stress activated protein Kinase2)

Cat. Num. AAA637521

• Gene Names: Mapk14; p38; Crk1; Mxi2; p38a; CSBP2; Csbp1; PRKM14; PRKM15; p38MAPK; p38alpha; p38-alpha
• Purity: Purified; Purified using Ni2+/NTA-agarose. Activated using MAP Kinase 2/Erk2, active and repurified using Ni2+/NTA-agarose. ~79% by SDS-PAGE and Coomassie blue staining.
• Synonyms: MSK2; active; Recombinant; Human (Mitogen and Stress activated protein Kinase2); Mapk14 active protein

• Host: Sf21 insect cells
• Purity/Purification: Purified; Purified using Ni2+/NTA-agarose. Activated using MAP Kinase 2/Erk2, active and repurified using Ni2+/NTA-agarose. ~79% by SDS-PAGE and Coomassie blue staining.
• Form/Format: Supplied as a liquid in 50mM Tris-HCl, pH 7.5, 300mM sodium chloride, 0.1mM EGTA, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol, 0.03% Brij-35 and 270mM sucrose.

• Application Notes: MS Tryptic Fingerprint: Confirmed identity as MSK2 with 37% amino acid coverage of the translated native sequence., MSK2 Kinase Assay: 10-100ng of this lot of enzyme phosphorylated 30uM Crosstide in the assay. Assay background was subtracted from the actual counts to yield the results.
• Specific Activity: ~140U/mg, where one unit of MSK2 activity is defined as 1nmol phosphate incorporated into 30uM Crosstide (GRPRTSSFAEG) per minute at 30 degree C with a final ATP concentration of 100uM.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for Mapk14 active protein

N-terminal His6-tagged, recombinant, human MSK2, aa2-end, expressed by baculovirus in Sf21 insect cells.

Product Categories/Family for Mapk14 active protein

Molecular Biology; MB-Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 1346566
• NCBI GeneID: 26416
• UniProt Accession #: P47811; O08666; Q3U6R5; Q3UZS3; Q8C289; Q9JLV8; Q9QZ80; B2KF37; B2KF38
• Molecular Weight: 41,287 Da
• NCBI Official Full Name: Mitogen-activated protein kinase 14
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 14
• NCBI Official Symbol: Mapk14
• NCBI Official Synonym Symbols: p38; Crk1; Mxi2; p38a; CSBP2; Csbp1; PRKM14; PRKM15; p38MAPK; p38alpha; p38-alpha
• NCBI Protein Information: mitogen-activated protein kinase 14; MAPK 14; p38 MAPK; p38 alpha; MAP kinase 14; MAP kinase p38 alpha; p38 MAP kinase alpha; tRNA synthetase cofactor p38; mitogen activated protein kinase 14; mitogen-activated protein kinase p38 alpha; cytokine suppressive anti-inflammatory drug binding protein 1
• UniProt Protein Name: Mitogen-activated protein kinase 14
• UniProt Gene Name: Mapk14
• UniProt Synonym Gene Names: Crk1; Csbp1; Csbp2; MAP kinase 14; MAPK 14
• UniProt Entry Name: MK14_MOUSE

Uniprot Description

P38A: a proline-directed ser/thr MAP kinase, and one of four p38 kinases that play important roles in cellular responses to inflammatory cytokines, DNA damage, oxidative stress, and some GPCRs, leading to direct activation of transcription factors and of other downstream kinases including MSK1, MSK2, eEF2K, MK2, and PRAK. MSK1 and -2 play important roles in the rapid induction of immediate-early genes in response to stress or mitogenic stimuli. MK2 and -3 control gene expression mostly at the post-transcriptional level. eEF2K is important for the elongation of mRNA during translation. Ectodomain shedding of transmembrane proteins is regulated by p38 MAPKs as well. In response to inflammatory stimuli, p38 MAPKs phosphorylate the membrane-associated metalloprotease ADAM17, which then cleaves the ectodomain of TGF-alpha family ligands, a process leading to the activation of EGFR signaling and cell proliferation. In the nucleus, many transcription factors are phosphorylated and activated by p38 MAPKs in response to different stimuli. Classical examples include ATF1, ATF2, ATF6, ELK1, PTPRH, CHOPO, p53 and MEF2C and MEF2A. The p38 MAPKs are emerging as important modulators of gene expression by regulating chromatin modifiers and remodelers. The promoters of several genes involved in the inflammatory response, such as IL6, IL8 and IL12B, display a p38 MAPK-dependent enrichment of histone H3 phosphorylation on 'Ser-10' (H3S10ph) in LPS-stimulated myeloid cells. Interacts directly with HDAC3 interacts directly and selectively to repress ATF2 transcriptional activity, and regulate TNF gene expression in LPS-stimulated cells. Phosphorylates the ubiquitin ligase SIAH2, regulating its activity towards EGLN3. May also inhibit the lysosomal degradation pathway of autophagy by interfering with the intracellular trafficking of the transmembrane protein ATG9. Regulates the endocytosis of membrane receptors that depend on RAB5A. Regulates the clathrin-mediated internalization of EGFR induced by inflammatory cytokines and UV irradiation by phosphorylating the EGFR and RAB5A effectors. Required in mid-fetal development for the growth of embryo-derived blood vessels in the labyrinth layer of the placenta. Plays an essential role in developmental and stress-induced erythropoiesis, through regulation of EPO gene expression. Interacts with casein kinase II subunits CSNK2A1 and CSNK2B. Activated by cell stresses such as DNA damage, heat shock, osmotic shock, anisomycin and sodium arsenite, as well as pro-inflammatory stimuli such as LPS and IL-1. Phosphorylated by ZAP70 in an alternative activation pathway in response to TCR signaling in T-cells, a pathway is inhibited by GADD45A. Four alternatively spliced isoforms of the human protein have been observed. Isoform MXI2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform EXIP may play a role in the early onset of apoptosis

Protein type: Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.24; CMGC group; MAPK family; p38 subfamily; MAPK/p38 subfamily

Cellular Component: nucleoplasm; spindle pole; mitochondrion; cell; cytoplasm; nucleus; cytosol

Molecular Function: transferase activity; MAP kinase activity; protein C-terminus binding; protein serine/threonine kinase activity; protein binding; NFAT protein binding; transferase activity, transferring phosphorus-containing groups; nucleotide binding; kinase activity; ATP binding; protein kinase activity

Biological Process: apoptosis; protein amino acid autophosphorylation; stress-activated MAPK cascade; cell morphogenesis; response to lipopolysaccharide; osteoclast differentiation; protein amino acid phosphorylation; positive regulation of glucose import; regulation of transcription, DNA-dependent; transmembrane receptor protein serine/threonine kinase signaling pathway; lipopolysaccharide-mediated signaling pathway; chondrocyte differentiation; angiogenesis; positive regulation of cardiac muscle cell proliferation; cartilage condensation; placenta development; skeletal muscle development; transcription, DNA-dependent; positive regulation of erythrocyte differentiation; DNA damage response, signal transduction; glucose metabolic process; positive regulation of myoblast differentiation; regulation of transcription from RNA polymerase II promoter; peptidyl-serine phosphorylation; positive regulation of protein import into nucleus; response to muramyl dipeptide; DNA damage checkpoint; striated muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; fatty acid oxidation; vascular endothelial growth factor receptor signaling pathway; phosphorylation; response to DNA damage stimulus; myoblast cell differentiation involved in skeletal muscle regeneration