Rabbit SETD2 Polyclonal Antibody | anti-SETD2 antibody

Phospho-SETD2 (Ser532) Antibody

Cat. Num. AAA9610414

• Gene Names: SETD2; LLS; HYPB; SET2; HIF-1; HIP-1; KMT3A; HBP231; HSPC069; p231HBP
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Dog
• Applications: Western Blot, Immunohistochemistry, ELISA
• Purity: Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Synonyms: SETD2; Polyclonal Antibody; Phospho-SETD2 (Ser532) Antibody; EC 2.1.1.43; FLJ16420; FLJ22472; FLJ23184; FLJ45883; HBP231; HIF 1; HIF-1; HIF1; HIP-1; Histone lysine N methyltransferase SETD2; Histone-lysine N-methyltransferase SETD2; hSET2; HSPC069; Huntingtin interacting protein 1; Huntingtin interacting protein; Huntingtin interacting protein B; Huntingtin interacting protein HYPB; Huntingtin yeast partner B; Huntingtin-binding protein; 231-KD; Huntingtin-interacting protein 1; Huntingtin-interacting protein B; HYPB; KIAA1732; KMT3A; Lysine N methyltransferase 3A; Lysine N-methyltransferase 3A; p231HBP; SET domain containing 2; SET domain-containing protein 2; SET2; SETD2_HUMAN; anti-SETD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted: Pig, Bovine, Horse, Sheep, Dog
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Phospho-SETD2(Ser532) Antibody detects endogenous levels of SETD2 only when phosphorylated at Ser532.
• Purity/Purification: Purified rabbit serum by affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 2564

• Applicable Applications for anti-SETD2 antibody: Western Blot (WB), Immunohistochemistry (IHC), Peptide ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ELISA (Peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human SETD2 around the phosphorylation site of Ser532.
• Tissue Specificity: Ubiquitously expressed.
• Subcellular Location: Nucleus
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Immunohistochemistry (IHC)

(At 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Immunohistochemmistry (IHC)

(At 1/100 staining rat liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Immunohistochemistry (IHC)

(At 1/100 staining mouse heart tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary)

Western Blot (WB)

(Western blot analysis of Phospho-SETD2(Ser532) in lysates of HeLa?, using Phospho-SETD2(Ser532) Antibody.)

Related Product Information for anti-SETD2 antibody

Description: Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate (PubMed:16118227, PubMed:19141475, PubMed:21526191, PubMed:21792193, PubMed:23043551, PubMed:27474439). Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation (By similarity). Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A (PubMed:23325844). Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction (PubMed:23622243). Required for DNA double-strand break repair in response to DNA damage: acts by mediating formation of H3K36me3, promoting recruitment of RAD51 and DNA repair via homologous recombination (HR) (PubMed:24843002). Acts as a tumor suppressor (PubMed:24509477). H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A (PubMed:27317772). H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase (PubMed:21792193). Required during angiogenesis (By similarity). Required for endoderm development by promoting embryonic stem cell differentiation toward endoderm: acts by mediating formation of H3K36me3 in distal promoter regions of FGFR3, leading to regulate transcription initiation of FGFR3 (By similarity). In addition to histones, also mediates methylation of other proteins, such as tubulins and STAT1 (PubMed:27518565, PubMed:28753426). Trimethylates 'Lys-40' of alpha-tubulins such as TUBA1B (alpha-TubK40me3); alpha-TubK40me3 is required for normal mitosis and cytokinesis and may be a specific tag in cytoskeletal remodeling (PubMed:27518565). Involved in interferon-alpha-induced antiviral defense by mediating both monomethylation of STAT1 at 'Lys-525' and catalyzing H3K36me3 on promoters of some interferon-stimulated genes (ISGs) to activate gene transcription (PubMed:28753426).

Post Translational Modifications: May be automethylated.

Subunit Structure: Specifically interacts with hyperphosphorylated C-terminal domain (CTD) of RNA polymerase II large subunit (POLR2A): binds to CTD heptad repeats doubly phosphorylated on 'Ser-2' and 'Ser-5' of each heptad (PubMed:16118227, PubMed:16314571). Interacts with HTT (PubMed:11461154, PubMed:9700202, PubMed:10958656). Interacts with IWS1 (PubMed:19141475). Interacts with p53/TP53; leading to regulate p53/TP53 target genes (PubMed:18585004). Component of a complex with HNRNPL (PubMed:19332550). Interacts with TUBA1A; the interaction is independent on alpha-tubulin acetylation on 'Lys-40' (PubMed:27518565). Interacts with STAT1 (PubMed:28753426).

Similarity: The low charge region mediates the transcriptional activation activity. The catalytic SET domain binds histone H3 (PubMed:27474439, PubMed:28256625). It is also able to bind oncogenic histone H3 K36M/I found in a number of cancer types, in which histone H3 'Lys-36' is replaced by a Met or an Ile residue. When binding the oncogenic variant histone H3 K36M/I, the SET domain undergoes dramatic conformational change to accommodate the histone H3 peptide, leading to sequester and inhibit SETD2 activity and block global H3K36 methylation (PubMed:27474439, PubMed:28256625). Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. SET2 subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 296452963
• NCBI GeneID: 29072
• UniProt Accession #: Q9BYW2
• Molecular Weight: Observed: 28 kDa; Predicted: 288 kDa
• NCBI Official Full Name: Histone-lysine N-methyltransferase SETD2
• NCBI Official Synonym Full Names: SET domain containing 2, histone lysine methyltransferase
• NCBI Official Symbol: SETD2
• NCBI Official Synonym Symbols: LLS; HYPB; SET2; HIF-1; HIP-1; KMT3A; HBP231; HSPC069; p231HBP
• NCBI Protein Information: histone-lysine N-methyltransferase SETD2
• UniProt Protein Name: Histone-lysine N-methyltransferase SETD2
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: SETD2
• UniProt Synonym Gene Names: HIF1; HYPB; KIAA1732; KMT3A; SET2; HIP-1; hSET2
• UniProt Entry Name: SETD2_HUMAN

NCBI Description

Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II. [provided by RefSeq, Aug 2008]

Uniprot Description

SETD2: Histone methyltransferase that methylates 'Lys-36' of histone H3. H3 'Lys-36' methylation represents a specific tag for epigenetic transcriptional activation. Probably plays a role in chromatin structure modulation during elongation via its interaction with hyperphosphorylated POLR2A. Binds DNA at promoters. May also act as a transcription activator that binds to promoters. Binds to the promoters of adenovirus 12 E1A gene in case of infection, possibly leading to regulate its expression. Belongs to the histone-lysine methyltransferase family. SET2 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - lysine degradation; Methyltransferase, protein lysine; EC 2.1.1.43; Methyltransferase

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: nucleoplasm; chromosome

Molecular Function: protein binding; histone-lysine N-methyltransferase activity

Biological Process: pericardium development; establishment and/or maintenance of chromatin architecture; stem cell development; mismatch repair; regulation of transcription, DNA-dependent; mesoderm morphogenesis; forebrain development; neural tube closure; RNA elongation from RNA polymerase II promoter; embryonic cranial skeleton morphogenesis; morphogenesis of a branching structure; angiogenesis