SDS-Page
(Recombinant SETD2 (1392-2564) protein gel. SETD2 (1392-2564) protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 134 kDa. Purity: > 70%)
SETD2 recombinant protein
Recombinant SETD2 (1392-2564) protein
Gene Names
SETD2; LLS; HYPB; SET2; HIF-1; HIP-1; KMT3A; HBP231; HSPC069; p231HBP
Purity
The recombinant protein is >85% pure by SDS-PAGE.
Synonyms
SETD2; Recombinant SETD2 (1392-2564) protein; HYPB; SET2; HIF-1; HIP-1; KMT3A; HBP231; HSPC069; p231HBP; SETD2 recombinant protein
Host
Baculovirus
Purity/Purification
The recombinant protein is >85% pure by SDS-PAGE.
Form/Format
Recombinant SETD2 (1392-2564) protein is supplied in 25mM HEPES- NaOH pH7.5, 300mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2mM TCEP.
Protein Species
Human
Tag
DYKDDDDK-Tag
Notes
Recombinant SETD2 (1392-2564) is suitable for use in the study of enzyme kinetics, inhibitor screening, and selectivity profiling. HMT Assay Conditions: 2ug recombinant nucleosomes were incubated with SETD2 (1392-2564) in reaction buffer for 3 hr at RT. Western blot was used to detect reaction products (H3K36me2 Antibody). SETD2 (1392-2564) alone was used as negative control.
Dry Ice Shipment
Extra charge fee may add to your shipping cost as dry ice is required to ship this product.
Preparation and Storage
Recombinant proteins in solution are temperature sensitive and must be stored at -80 degree C to prevent degradation. Avoid repeated freeze/thaw cycles and keep on ice when not in storage.
Shipping Temp: Dry Ice
Shipping Temp: Dry Ice
SDS-Page
(Recombinant SETD2 (1392-2564) protein gel. SETD2 (1392-2564) protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 134 kDa. Purity: > 70%)
SDS-Page
(Recombinant SETD2 (1392-2564) protein gel. SETD2 (1392-2564) protein was run on an 8% SDS-PAGE gel and stained with Coomassie Blue. MW: 134 kDa. Purity: > 70%)
Western Blot (WB)
(HMT Assay for Recombinant SETD2 (1392-2564) protein activity. 2 ug recombinant nucleosomes were incubated with SETD2 (1392-2564) in reaction buffer for 3 hr at RT. Western blot was used to detect reaction products (H3K36me2 Antibody). SETD2 (1392-2564) alone was used as negative control.)
Western Blot (WB)
(HMT Assay for Recombinant SETD2 (1392-2564) protein activity. 2 ug recombinant nucleosomes were incubated with SETD2 (1392-2564) in reaction buffer for 3 hr at RT. Western blot was used to detect reaction products (H3K36me2 Antibody). SETD2 (1392-2564) alone was used as negative control.)
Related Product Information for SETD2 recombinant protein
Short Description: Recombinant SETD2 (1392-2564)(accession number NP_054878.5) was expressed in Sf9 cells and contains an N-terminal DYKDDDDK tag with an observed molecular weight of 134 kDa. SETD2 (SET Domain Containing 2) is a histone methyltransferase that methylates histone H3 at lysine 36. H3K36 methylation is associated with active chromatin. SETD2 also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA pol II. Recombinant SETD2 (1392-2564) includes amino acids 1392-2564 that contains the SET domain of SETD2 that catalyzes methylation of lysine residues. Recombinant SETD2 (1392-2564) can be used to study enzyme kinetics and perform inhibitor screening.
Background: SETD2 (SET Domain Containing 2) is a histone methyltransferase that methylates histone H3 at lysine 36. H3K36 methylation is associated with active chromatin. SETD2 also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA pol II. Recombinant SETD2 (1392-2564) includes amino acids 1392-2564 that contains the SET domain of SETD2 that catalyzes methylation of lysine residues.
Background: SETD2 (SET Domain Containing 2) is a histone methyltransferase that methylates histone H3 at lysine 36. H3K36 methylation is associated with active chromatin. SETD2 also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA pol II. Recombinant SETD2 (1392-2564) includes amino acids 1392-2564 that contains the SET domain of SETD2 that catalyzes methylation of lysine residues.
Product Categories/Family for SETD2 recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
175,982 Da
NCBI Official Full Name
histone-lysine N-methyltransferase SETD2
NCBI Official Synonym Full Names
SET domain containing 2
NCBI Official Symbol
SETD2
NCBI Official Synonym Symbols
LLS; HYPB; SET2; HIF-1; HIP-1; KMT3A; HBP231; HSPC069; p231HBP
NCBI Protein Information
histone-lysine N-methyltransferase SETD2
Protein Family
NCBI Description
Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II. [provided by RefSeq, Aug 2008]
Research Articles on SETD2
Similar Products
Product Notes
The SETD2 (Catalog #AAA388118) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "SETD2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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