Rabbit SETD2 Polyclonal Antibody | anti-SETD2 antibody

Anti-SETD2 Antibody

Cat. Num. AAA2401294

• Gene Names: SETD2; LLS; HYPB; SET2; HIF-1; HIP-1; KMT3A; HBP231; HSPC069; p231HBP
• Reactivity: Human; Predicted: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: SETD2; Polyclonal Antibody; Anti-SETD2 Antibody; Rabbit Polyclonal (IgG) to Human SETD2; HBP231; HIF-1; HIP-1; HSPC069; KMT3A; Lysine N-methyltransferase 3A; HIF1; HSET2; HYPB; p231HBP; SET2; Huntingtin yeast partner B; KIAA1732; SET domain containing 2; anti-SETD2 antibody

• Host: Rabbit
• Reactivity: Human; Predicted: Mouse, Rat
• Clonality: Polyclonal
• Specificity: Human SETD2
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide, 50% glycerol.
• Sequence Length: 2564

• Applicable Applications for anti-SETD2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB)
• Application Notes: IHC-P: 5 ug/ml; WB: 1:500-1:2000
• Immunogen: Recombinant full length Human SETD2.
• Conjugation: Unconjugated
• Preparation and Storage: Store at 4 degree C for short term.; Aliquot and store at -20 degree C for long term.; Avoid freeze-thaw cycles.

Immunohistochemistry (IHC)

(Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))

Immunohistochemistry (WB)

(Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE))

Related Product Information for anti-SETD2 antibody

Histone methyltransferase that specifically trimethylates 'Lys-36' ofhistone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) assubstrate. Represents the main enzyme generating H3K36me3, a specifictag for epigenetic transcriptional activation. Plays a role in chromatinstructure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A.

NCBI and Uniprot Product Information

• NCBI GI #: 197313748
• NCBI GeneID: 29072
• NCBI Accession #: NP_054878.5
• NCBI GenBank Nucleotide #: NM_014159.6
• UniProt Accession #: Q9BYW2; O75397; O75405; Q17RW8; Q5BKS9; Q5QGN2; Q69YI5; Q6IN64; Q6ZN53; Q6ZS25; Q8N3R0; Q8TCN0
• Molecular Weight: 175,982 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase SETD2 isoform 1
• NCBI Official Synonym Full Names: SET domain containing 2
• NCBI Official Symbol: SETD2
• NCBI Official Synonym Symbols: LLS; HYPB; SET2; HIF-1; HIP-1; KMT3A; HBP231; HSPC069; p231HBP
• NCBI Protein Information: histone-lysine N-methyltransferase SETD2
• UniProt Protein Name: Histone-lysine N-methyltransferase SETD2
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: SETD2
• UniProt Synonym Gene Names: hSET2

NCBI Description

Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein belonging to a class of huntingtin interacting proteins characterized by WW motifs. This protein is a histone methyltransferase that is specific for lysine-36 of histone H3, and methylation of this residue is associated with active chromatin. This protein also contains a novel transcriptional activation domain and has been found associated with hyperphosphorylated RNA polymerase II. [provided by RefSeq, Aug 2008]

Uniprot Description

Histone methyltransferase that specifically trimethylates 'Lys-36' of histone H3 (H3K36me3) using dimethylated 'Lys-36' (H3K36me2) as substrate (PubMed:16118227, PubMed:19141475, PubMed:21526191, PubMed:21792193, PubMed:23043551, PubMed:27474439). Represents the main enzyme generating H3K36me3, a specific tag for epigenetic transcriptional activation (). Plays a role in chromatin structure modulation during elongation by coordinating recruitment of the FACT complex and by interacting with hyperphosphorylated POLR2A (PubMed:23325844). Acts as a key regulator of DNA mismatch repair in G1 and early S phase by generating H3K36me3, a mark required to recruit MSH6 subunit of the MutS alpha complex: early recruitment of the MutS alpha complex to chromatin to be replicated allows a quick identification of mismatch DNA to initiate the mismatch repair reaction (PubMed:23622243). Required for DNA double-strand break repair in response to DNA damage: acts by mediating formation of H3K36me3, promoting recruitment of RAD51 and DNA repair via homologous recombination (HR) (PubMed:24843002). Acts as a tumor suppressor (PubMed:24509477). H3K36me3 also plays an essential role in the maintenance of a heterochromatic state, by recruiting DNA methyltransferase DNMT3A (PubMed:27317772). H3K36me3 is also enhanced in intron-containing genes, suggesting that SETD2 recruitment is enhanced by splicing and that splicing is coupled to recruitment of elongating RNA polymerase (PubMed:21792193). Required during angiogenesis (). Required for endoderm development by promoting embryonic stem cell differentiation toward endoderm: acts by mediating formation of H3K36me3 in distal promoter regions of FGFR3, leading to regulate transcription initiation of FGFR3 (). In addition to histones, also mediates methylation of other proteins, such as tubulins and STAT1 (PubMed:27518565, PubMed:28753426). Trimethylates 'Lys-40' of alpha-tubulins such as TUBA1B (alpha-TubK40me3); alpha-TubK40me3 is required for normal mitosis and cytokinesis and may be a specific tag in cytoskeletal remodeling (PubMed:27518565). Involved in interferon-alpha-induced antiviral defense by mediating both monomethylation of STAT1 at 'Lys-525' and catalyzing H3K36me3 on promoters of some interferon-stimulated genes (ISGs) to activate gene transcription (PubMed:28753426).