SerpinA6 / CBG Recombinant Protein | CBG recombinant protein

Recombinant Human SerpinA6 / CBG Protein (His tag)

Cat. Num. AAA2545971

• Gene Names: SERPINA6; CBG
• Purity: > 98 % as determined by SDS-PAGE
• Synonyms: SerpinA6 / CBG; Recombinant Human SerpinA6 / CBG Protein (His tag); CBG; AI265318; AV104445; CBG recombinant protein

• Host: Human Cells
• Purity/Purification: > 98 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% trehalose, 5% mannitol, 0.01% Tween80.
• Sequence Length: 405

• Application Notes: The secreted recombinant human SERPINA6 consists of 394 amino acids and predictes a molecular mass of 44 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of the protein is approximately 55-60 kDa due to glycosylation.
• Predicted N Terminal: Met 23
• Source: Human
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method
• Reconstitution: It is recommended that sterile water(400uL) be added to the vial to prepare a stock solutin of 0.25 mg/mL.; Concentration is measured by UV-Vis
• Preparation and Storage: Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted and be used as soon as possible. Avoid repeated freeze-thaw cycles.

Testing Data (TD)

Related Product Information for CBG recombinant protein

Background: Corticosteroid-binding globulin (CBG), also known as SerpinA6, is a non-inhibitory member of the serine proteinase inhibitor (serpin) superfamily. It is the high-affinity transport protein for glucocorticoids in vertebrate blood. CBG is specifically cleaved by this protease at a precise site close to its carboxy-terminus. This induces a conformation change and disrupts the binding between glucocorticoids and CBG, and promotes a significant and local release of glucocorticoids (over 90% of them are bound to CBG in human plasma). In this context, CBG directs glucocorticoids to sites of inflammation, and plays in consequence a crucial role in efficient glucocorticoid action in physiology. The SerpinA6 protein is mainly secreted by the liver. This negative acute phase protein regulates free cortisol levels in the blood and distributes cortisol to its target tissues. SerpinA6 deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo-/hypertension and muscle fatigue. There are three heritable, human CBG gene mutations that can reduce CBG-cortisol binding affinity and/or reduce circulating CBG levels.

Description: A DNA sequence encoding the human SERPINA6 (NP_001747.2) precursor (Met 1-Val 405) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 73858564
• NCBI GeneID: 866
• NCBI Accession #: NP_001747.2
• NCBI GenBank Nucleotide #: NM_001756.3
• UniProt Accession #: P08185; Q7Z2Q9; A8K456
• NCBI Official Full Name: corticosteroid-binding globulin
• NCBI Official Synonym Full Names: serpin family A member 6
• NCBI Official Symbol: SERPINA6
• NCBI Official Synonym Symbols: CBG
• NCBI Protein Information: corticosteroid-binding globulin
• UniProt Protein Name: Corticosteroid-binding globulin
• UniProt Gene Name: SERPINA6
• UniProt Synonym Gene Names: CBG; CBG

NCBI Description

This gene encodes an alpha-globulin protein with corticosteroid-binding properties. This is the major transport protein for glucorticoids and progestins in the blood of most vertebrates. The gene localizes to a chromosomal region containing several closely related serine protease inhibitors which may have evolved by duplication events. [provided by RefSeq, Jul 2008]

Uniprot Description

SERPINA6: Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species. Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency). CBG deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo/hypertension and muscle fatigue. Belongs to the serpin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 14q32.13

Cellular Component: extracellular space

Molecular Function: serine-type endopeptidase inhibitor activity; steroid binding

Disease: Corticosteroid-binding Globulin Deficiency