Western Blot (WB)
(Western blot detection of Kaiso in MCF7,C6 cell lysates using Kaiso Rabbit pAb(1:1000 diluted). Predicted band size:75KDa. Observed band size:90KDa.)
Rabbit Kaiso Polyclonal Antibody | anti-ZBTB33 antibody
Kaiso Rabbit pAb
Gene Names
ZBTB33; ZNF348; ZNF-kaiso
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Affinity purified
Synonyms
Kaiso; Polyclonal Antibody; Kaiso Rabbit pAb; Transcriptional regulator Kaiso; ZNF348; ZNF-kaiso; anti-ZBTB33 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Expressed in vascular endothelium.
Purity/Purification
Affinity purified
Form/Format
Supplied in 50nM Tris-Glycine (pH7.4), 0.15M Nacl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
Applicable Applications for anti-ZBTB33 antibody
Western Blot (WB)
Application Notes
WB: 1:1000-1:5000
Immunogen
A synthetic peptide of human Kaiso
Conjugation
Unconjugated
Modification
Unmodification
Subcellular Location
Cytoplasm, Nucleus
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot detection of Kaiso in MCF7,C6 cell lysates using Kaiso Rabbit pAb(1:1000 diluted). Predicted band size:75KDa. Observed band size:90KDa.)
Western Blot (WB)
(Western blot detection of Kaiso in MCF7,C6 cell lysates using Kaiso Rabbit pAb(1:1000 diluted). Predicted band size:75KDa. Observed band size:90KDa.)
Related Product Information for anti-ZBTB33 antibody
This gene encodes a transcriptional regulator with bimodal DNA-binding specificity, which binds to methylated CGCG and also to the non-methylated consensus KAISO-binding site TCCTGCNA. The protein contains an N-terminal POZ/BTB domain and 3 C-terminal zinc finger motifs. It recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. It may contribute to the repression of target genes of the Wnt signaling pathway, and may also activate transcription of a subset of target genes by the recruitment of catenin delta-2 (CTNND2). Its interaction with catenin delta-1 (CTNND1) inhibits binding to both methylated and non-methylated DNA. It also interacts directly with the nuclear import receptor Importin-alpha2 (also known as karyopherin alpha2 or RAG cohort 1), which may mediate nuclear import of this protein. Alternatively spliced transcript variants encoding the same protein have been identified.[provided by RefSeq, May 2010]
Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1 (PubMed:23251453).
Transcriptional regulator with bimodal DNA-binding specificity. Binds to methylated CpG dinucleotides in the consensus sequence 5'-CGCG-3' and also binds to the non-methylated consensus sequence 5'-CTGCNA-3' also known as the consensus kaiso binding site (KBS). Recruits the N-CoR repressor complex to promote histone deacetylation and the formation of repressive chromatin structures in target gene promoters. May contribute to the repression of target genes of the Wnt signaling pathway. May also activate transcription of a subset of target genes by the recruitment of CTNND2. Represses expression of MMP7 in conjunction with transcriptional corepressors CBFA2T3, CBFA2T2 and RUNX1T1 (PubMed:23251453).
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
74,484 Da
NCBI Official Full Name
transcriptional regulator Kaiso
NCBI Official Synonym Full Names
zinc finger and BTB domain containing 33
NCBI Official Symbol
ZBTB33
NCBI Official Synonym Symbols
ZNF348; ZNF-kaiso
NCBI Protein Information
transcriptional regulator Kaiso; WUGSC:H_DJ525N14.1; kaiso transcription factor; zinc finger and BTB domain-containing protein 33
UniProt Protein Name
Transcriptional regulator Kaiso
Protein Family
UniProt Gene Name
ZBTB33
UniProt Synonym Gene Names
KAISO; ZNF348
UniProt Entry Name
KAISO_HUMAN
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Product Notes
The ZBTB33 zbtb33 (Catalog #AAA4752048) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Kaiso Rabbit pAb reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Kaiso can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:1000-1:5000. Researchers should empirically determine the suitability of the ZBTB33 zbtb33 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kaiso, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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