Monkey Semaphorin 7A ELISA Kit | SEMA7A elisa kit

Monkey Semaphorin 7A ELISA Kit

Cat. Num. AAA737035

• Gene Names: SEMA7A; JMH; CD108; SEMAL; CDw108; SEMAK1; H-Sema-L; H-SEMA-K1
• Reactivity: Monkey
• Synonyms: Semaphorin 7A; Monkey Semaphorin 7A ELISA Kit; SEMA7A elisa kit

• Reactivity: Monkey

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Sandwich
• Detection Range: 1.0-25ng/mL
• Sensitivity: 0.1ng/mL
• Intended Uses: This SEMA7A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Monkey SEMA7A. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SEMA7A elisa kit

Principle of the assay: SEMA7A ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SEMA7A. Standards or samples are then added to the microtiter plate wells and SEMA7A if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SEMA7A present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for SEMA7A are added to each well to “sandwich” the SEMA7A immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SEMA7A and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SEMA7A concentration in each sample is interpolated from this standard curve.

Product Categories/Family for SEMA7A elisa kit

Immunology

NCBI and Uniprot Product Information

• NCBI GI #: 225637524
• NCBI GeneID: 8482
• NCBI Accession #: NP_001139502.1
• NCBI GenBank Nucleotide #: NM_001146030.1
• Molecular Weight: 73,364 Da
• NCBI Official Full Name: semaphorin-7A isoform 3
• NCBI Official Synonym Full Names: semaphorin 7A, GPI membrane anchor (John Milton Hagen blood group)
• NCBI Official Symbol: SEMA7A
• NCBI Official Synonym Symbols: JMH; CD108; SEMAL; CDw108; SEMAK1; H-Sema-L; H-SEMA-K1
• NCBI Protein Information: semaphorin-7A; sema L; sema K1; semaphorin L; semaphorin-L; semaphorin K1; semaphorin-K1; JMH blood group antigen; John Milton Hagen blood group H-Sema K1; john-Milton-Hargen human blood group Ag; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, 7A; sema domain, immunoglobulin domain (Ig), and GPI membrane anchor, (semaphorin) 7A (JMH blood group)
• UniProt Protein Name: Semaphorin-7A
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA7A
• UniProt Synonym Gene Names: CD108; SEMAL; Sema K1; Sema L
• UniProt Entry Name: SEM7A_HUMAN

NCBI Description

The protein encoded by this gene binds to cell surfaces through a glycosylphosphatidylinositol (GPI) linkage. The encoded glycoprotein is found on activated lymphocytes and erythrocytes. This protein may be involved in immunomodulatory and neuronal processes. Defects in this gene can result in loss of bone mineral density (BMD). Three transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Mar 2009]

Uniprot Description

SEMA7A: Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. Belongs to the semaphorin family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 15q22.3-q23

Cellular Component: membrane; plasma membrane; external side of plasma membrane

Molecular Function: integrin binding; protein binding; receptor activity

Biological Process: integrin-mediated signaling pathway; osteoblast differentiation; axon guidance; axon extension; positive regulation of axon extension; regulation of inflammatory response; olfactory lobe development; immune response; positive regulation of protein amino acid phosphorylation; inflammatory response

Disease: Blood Group, John Milton Hagen System