Human Semaphorin 7A ELISA Kit | SEMA7A elisa kit

Human Semaphorin 7A ELISA Kit

Cat. Num. AAA762459

• Gene Names: SEMA7A; JMH; CD108; SEMAL; CDw108; SEMAK1; H-Sema-L; H-SEMA-K1
• Reactivity: Human
• Synonyms: Semaphorin 7A; Human Semaphorin 7A ELISA Kit; SEMA7A/CD108/H-Sema-L/Sema7A/SEMAL/CD108 antigen/CD108MGC126696/CDW108/CDw108/H-SEMA-K1/H-Sema-L/JMH/JMH blood group antigen/John-Milton-Hargen human blood group Ag/sema domain; immunoglobulin domain (Ig); and GPI membrane anchor; (semaphorin)7A/sema domain; (semaphorin)7A (JMH blood group)/sema domain; 7A/Sema K1/Sema L/SEMAK1/SEMALJohn Milton Hagen blood group H-Sema K1/semaphorin 7A; GPI membrane anchor (John Milton Hagen blood group)/semaphorin K1/semaphorin L/semaphorin-7A/semaphorin-K1/Semaphorin-K1/semaphorin-L/Semaphorin-L; SEMA7A elisa kit

• Reactivity: Human
• Sequence Length: 652

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for SEMA7A elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human SEMA7A antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human SEMA7A antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human SEMA7A amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human SEMA7A can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 225637520
• NCBI GeneID: 8482
• NCBI Accession #: NP_001139501.1
• NCBI GenBank Nucleotide #: NM_001146029.2
• Molecular Weight: 73,364 Da
• NCBI Official Full Name: semaphorin-7A isoform 2
• NCBI Official Synonym Full Names: semaphorin 7A (John Milton Hagen blood group)
• NCBI Official Symbol: SEMA7A
• NCBI Official Synonym Symbols: JMH; CD108; SEMAL; CDw108; SEMAK1; H-Sema-L; H-SEMA-K1
• NCBI Protein Information: semaphorin-7A
• UniProt Protein Name: Semaphorin-7A
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA7A
• UniProt Synonym Gene Names: CD108; SEMAL; Sema K1; Sema L
• UniProt Entry Name: SEM7A_HUMAN

NCBI Description

This gene encodes a member of the semaphorin family of proteins. The encoded preproprotein is proteolytically processed to generate the mature glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein. The encoded protein is found on activated lymphocytes and erythrocytes and may be involved in immunomodulatory and neuronal processes. The encoded protein carries the John Milton Hagen (JMH) blood group antigens. Mutations in this gene may be associated with reduced bone mineral density (BMD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

Uniprot Description

SEMA7A: Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes. Belongs to the semaphorin family.

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 15q22.3-q23

Cellular Component: external side of plasma membrane; extracellular space; membrane; plasma membrane

Molecular Function: chemorepellent activity; integrin binding; protein binding; semaphorin receptor binding

Biological Process: axon extension; axon guidance; immune response; inflammatory response; integrin-mediated signaling pathway; negative chemotaxis; negative regulation of axon extension involved in axon guidance; neural crest cell migration; olfactory lobe development; osteoblast differentiation; positive regulation of axon extension; positive regulation of cell migration; regulation of inflammatory response

Disease: Blood Group, John Milton Hagen System