Human Semaphorin 7A (SEMA7A) ELISA Kit | SEMA7A elisa kit

Human Semaphorin 7A (SEMA7A) ELISA Kit

Cat. Num. AAA453967

• Gene Names: SEMA7A; JMH; CD108; SEMAL; CDw108; SEMAK1; H-Sema-L; H-SEMA-K1
• Reactivity: Human
• Synonyms: Semaphorin 7A (SEMA7A); Human Semaphorin 7A (SEMA7A) ELISA Kit; CD108; CDw108; H-SEMA-K1; H-Sema-L; JMH; SEMAK1; SEMAL; Semaphorin 7A; GPI Membrane Anchor; John Milton Hagen Blood Group; Semaphorin-K1; Semaphorin-L; SEMA7A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of SEMA7A. No significant cross-reactivity or interference between SEMA7A and analogues was observed.
• Sequence Length: 652

• Samples: Serum, plasma, tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.059ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level SEMA7A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level SEMA7A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SEMA7A elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of SEMA7A in human serum, plasma, tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SEMA7A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to SEMA7A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SEMA7A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SEMA7A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 225637520
• NCBI GeneID: 8482
• NCBI Accession #: NP_001139501.1
• NCBI GenBank Nucleotide #: NM_001146029.2
• UniProt Accession #: O75326; Q1XE81; Q1XE82; Q1XE83; Q1XE84; Q3MIY5; B4DDP7; F5H1S0
• Molecular Weight: 73,364 Da
• NCBI Official Full Name: semaphorin-7A isoform 2
• NCBI Official Synonym Full Names: semaphorin 7A (John Milton Hagen blood group)
• NCBI Official Symbol: SEMA7A
• NCBI Official Synonym Symbols: JMH; CD108; SEMAL; CDw108; SEMAK1; H-Sema-L; H-SEMA-K1
• NCBI Protein Information: semaphorin-7A
• UniProt Protein Name: Semaphorin-7A
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA7A
• UniProt Synonym Gene Names: CD108; SEMAL; Sema K1; Sema L

NCBI Description

This gene encodes a member of the semaphorin family of proteins. The encoded preproprotein is proteolytically processed to generate the mature glycosylphosphatidylinositol (GPI)-anchored membrane glycoprotein. The encoded protein is found on activated lymphocytes and erythrocytes and may be involved in immunomodulatory and neuronal processes. The encoded protein carries the John Milton Hagen (JMH) blood group antigens. Mutations in this gene may be associated with reduced bone mineral density (BMD). Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016]

Uniprot Description

Plays an important role in integrin-mediated signaling and functions both in regulating cell migration and immune responses. Promotes formation of focal adhesion complexes, activation of the protein kinase PTK2/FAK1 and subsequent phosphorylation of MAPK1 and MAPK3. Promotes production of proinflammatory cytokines by monocytes and macrophages. Plays an important role in modulating inflammation and T-cell-mediated immune responses. Promotes axon growth in the embryonic olfactory bulb. Promotes attachment, spreading and dendrite outgrowth in melanocytes.