Mouse anti-Human SULT2A1 Monoclonal Antibody | anti-SULT2A1 antibody

Anti-SULT2A1 Antibody, Mouse Monoclonal

Cat. Num. AAA8105312

• Gene Names: SULT2A1; HST; ST2; STD; hSTa; DHEAS; ST2A1; ST2A3; DHEA-ST
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Protein A
• Synonyms: SULT2A1; Monoclonal Antibody; Anti-SULT2A1 Antibody; Mouse Monoclonal; SULT2A1/STD Antibody; Mouse MAb; sulfotransferase family; cytosolic; 2A; dehydroepiandrosterone (DHEA)-preferring; member 1; Anti-DHEA-ST Antibody; Anti-DHEAS Antibody; Anti-HST Antibody; Anti-hSTa Antibody; Anti-ST2 Antibody; Anti-ST2A1 Antibody; Anti-ST2A3 Antibody; Anti-STD Antibody; anti-SULT2A1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 9G8G12G10
• Specificity: Human SULT2A1/STD.; No cross-reactivity in ELISA with Human SULT1B1
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-SULT2A1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; ELISA: 1:1000-1:2000
• Immunogen: Recombinant Human SULT2A1 protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human SULT2A1 (rh SULT2A1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-SULT2A1 mouse monoclonal antibody at 1:500 dilutionLane A: HepG2 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:34 kDaObserved band size:35 kDa)

Related Product Information for anti-SULT2A1 antibody

Hydroxysteroid sulfotransferase (SULT2A1) is a key enzyme in the testicular and hepatic metabolism of 5alpha-androstenone, which is a major component of the off-odor and off-flavor in pork known as boar taint. Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate specificities. The gene structure (number and length of exons) is similar among family members. SULT2A1 is a sulfo-conjugating phase II enzyme expressed at very high levels in the liver and intestine, the two major first-pass metabolic tissues, and in the steroidogenic adrenal tissue. SULT2A1 acts preferentially on the hydroxysteroids dehydroepiandrosterone, testosterone/dihydrotestosterone, and pregnenolone and on cholesterol-derived amphipathic sterol bile acids.

References

Chatterjee, B. 2005, Methods Enzymol. 400:165-91. Liu, Y. et al., 2006, Chem Res Toxicol. 19 (11):1420-5. Sinclair, PA. et al., 2006, J Mol Endocrinol 36 (2):301-11. Yalcin, EB. et al., 2008, Drug Metab Lett 2 (3):198-204.

NCBI and Uniprot Product Information

• NCBI GI #: 29540545
• NCBI GeneID: 6822
• NCBI Accession #: NP_003158.2
• NCBI GenBank Nucleotide #: NM_003167.3
• UniProt Accession #: Q06520
• Molecular Weight: 33,780 Da
• NCBI Official Full Name: bile salt sulfotransferase
• NCBI Official Synonym Full Names: sulfotransferase family, cytosolic, 2A, dehydroepiandrosterone (DHEA)-preferring, member 1
• NCBI Official Symbol: SULT2A1
• NCBI Official Synonym Symbols: HST; ST2; STD; hSTa; DHEAS; ST2A1; ST2A3; DHEA-ST
• NCBI Protein Information: bile salt sulfotransferase; alcohol/hydroxysteroid sulfotransferase; bile-salt sulfotranasferase 2A1
• UniProt Protein Name: Bile salt sulfotransferase
• Protein Family: Bile salt sulfotransferase
• UniProt Gene Name: SULT2A1
• UniProt Synonym Gene Names: HST; STD; DHEA-ST; HST; ST2A1
• UniProt Entry Name: ST2A1_HUMAN

NCBI Description

This gene encodes a member of the sulfotransferase family. Sulfotransferases aid in the metabolism of drugs and endogenous compounds by converting these substances into more hydrophilic water-soluble sulfate conjugates that can be easily excreted. This protein catalyzes the sulfation of steroids and bile acids in the liver and adrenal glands, and may have a role in the inherited adrenal androgen excess in women with polycystic ovary syndrome. [provided by RefSeq, Mar 2010]

Uniprot Description

SULT2A1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfonation of steroids and bile acids in the liver and adrenal glands. Belongs to the sulfotransferase 1 family.

Protein type: EC 2.8.2.14; Transferase

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: cytosol

Molecular Function: sulfotransferase activity; protein binding; bile-salt sulfotransferase activity

Biological Process: steroid metabolic process; bile acid catabolic process; xenobiotic metabolic process; digestion; sulfation; cellular lipid metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process