SDS-Page
(Recombinant Human Semaphorin-5A Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 100 kDa.)
Semaphorin-5A Recombinant Protein | SEMA5A recombinant protein
Recombinant Human Semaphorin-5A Protein
Gene Names
SEMA5A; semF; SEMAF
Purity
>95% by SDS-PAGE.
Synonyms
Semaphorin-5A; Recombinant Human Semaphorin-5A Protein; Semaphorin-F; Sema F; SEMAF; SEMA5A recombinant protein
Host
Mammalian
Purity/Purification
>95% by SDS-PAGE.
Form/Format
Lyophilized from a 0.22 um filtered solution of 20mM PB, 150mM NaCl, 0.1mM EDTA, 0.05% Tween 20, pH 7.2.
Sequence
EAQGTTQCQRTEHPVISYKEIGPWLREFRAKNAVDFSQLTFDPGQKELVVGARNYLFRLQLEDLSLIQAVEWECDEATKKACYSKGKSKEECQNYIRVLLVGGDRLFTCGTNAFTPVCTNRSLSNLTEIHDQISGMARCPYSPQHNSTALLTAGGELYAATAMDFPGRDPAIYRSLGILPPLRTAQYNSKWLNEPNFVSSYDIGNFTYFFFRENAVEHDCGKTVFSRAARVCKNDIGGRFLLEDTWTTFMKARLNCSRPGEVPFYYNELQSTFFLPELDLIYGIFTTNVNSIAASAVCVFNLSAIAQAFSGPFKYQENSRSAWLPYPNPNPHFQCGTVDQGLYVNLTERNLQDAQKFILMHEVVQPVTTVPSFMEDNSRFSHVAVDVVQGREALVHIIYLATDYGTIKKVRVPLNQTSSSCLLEEIELFPERRREPIRSLQILHSQSVLFVGLREHVVKIPLKRCQFYRTRSTCIGAQDPYCGWDVVMKKCTSLEESLSMTQWEQSISACPTRNLTVDGHFGVWSPWTPCTHTDGSAVGSCLCRTRSCDSPAPQCGGWQCEGPGMEIANCSRNGGWTPWTSWSPCSTTCGIGFQVRQRSCSNPTPRHGGRVCVGQNREERYCNEHLLCPPHMFWTGWGPWERCTAQCGGGIQARRRICENGPDCAGCNVEYQSCNTNPCPELKKTTPWTPWTPVNISDNGGHYEQRFRYTCKARLADPNLLEVGRQRIEMRYCSSDGTSGCST
Sequence Length
1074
Species
Human
Endotoxin
< 1.0 EU/ug of the protein by LAL method.
Reconstitution
Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.
Tag
6xHis tag at the C-terminus
Preparation and Storage
Store the lyophilized protein at -20 degree C to -80 degree C for long term.
After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week.
After reconstitution, the protein solution is stable at -20 degree C for 3 months, at 2-8 degree C for up to 1 week.
SDS-Page
(Recombinant Human Semaphorin-5A Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 100 kDa.)
SDS-Page
(Recombinant Human Semaphorin-5A Protein was determined by SDS-PAGE with Coomassie Blue, showing a band at 100 kDa.)
Related Product Information for SEMA5A recombinant protein
Description: Recombinant Human Semaphorin-5A Protein is produced by Mammalian expression system. The target protein is expressed with sequence (Glu23-Thr765) of human Semaphorin-5A (Accession #Q13591) fused with a 6xHis tag at the C-terminus.
Background: This protein is a mbrane protein containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.
Background: This protein is a mbrane protein containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.
Product Categories/Family for SEMA5A recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
semaphorin-5A
NCBI Official Synonym Full Names
semaphorin 5A
NCBI Official Symbol
SEMA5A
NCBI Official Synonym Symbols
semF; SEMAF
NCBI Protein Information
semaphorin-5A
UniProt Protein Name
Semaphorin-5A
Protein Family
UniProt Gene Name
SEMA5A
UniProt Synonym Gene Names
SEMAF; Sema F
NCBI Description
This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.[provided by RefSeq, Jan 2010]
Uniprot Description
Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.
Research Articles on SEMA5A
Similar Products
Product Notes
The SEMA5A sema5a (Catalog #AAA9141908) is a Recombinant Protein produced from Mammalian and is intended for research purposes only. The product is available for immediate purchase. The amino acid sequence is listed below: EAQGTTQCQR TEHPVISYKE IGPWLREFRA KNAVDFSQLT FDPGQKELVV GARNYLFRLQ LEDLSLIQAV EWECDEATKK ACYSKGKSKE ECQNYIRVLL VGGDRLFTCG TNAFTPVCTN RSLSNLTEIH DQISGMARCP YSPQHNSTAL LTAGGELYAA TAMDFPGRDP AIYRSLGILP PLRTAQYNSK WLNEPNFVSS YDIGNFTYFF FRENAVEHDC GKTVFSRAAR VCKNDIGGRF LLEDTWTTFM KARLNCSRPG EVPFYYNELQ STFFLPELDL IYGIFTTNVN SIAASAVCVF NLSAIAQAFS GPFKYQENSR SAWLPYPNPN PHFQCGTVDQ GLYVNLTERN LQDAQKFILM HEVVQPVTTV PSFMEDNSRF SHVAVDVVQG REALVHIIYL ATDYGTIKKV RVPLNQTSSS CLLEEIELFP ERRREPIRSL QILHSQSVLF VGLREHVVKI PLKRCQFYRT RSTCIGAQDP YCGWDVVMKK CTSLEESLSM TQWEQSISAC PTRNLTVDGH FGVWSPWTPC THTDGSAVGS CLCRTRSCDS PAPQCGGWQC EGPGMEIANC SRNGGWTPWT SWSPCSTTCG IGFQVRQRSC SNPTPRHGGR VCVGQNREER YCNEHLLCPP HMFWTGWGPW ERCTAQCGGG IQARRRICEN GPDCAGCNVE YQSCNTNPCP ELKKTTPWTP WTPVNISDNG GHYEQRFRYT CKARLADPNL LEVGRQRIEM RYCSSDGTSG CST. It is sometimes possible for the material contained within the vial of "Semaphorin-5A, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
