Semaphorin 5A / SEMA5A Recombinant Protein | SEMA5A recombinant protein

Recombinant Human Semaphorin 5A / SEMA5A Protein (His tag)

Cat. Num. AAA2545821

• Gene Names: SEMA5A; semF; SEMAF
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: Semaphorin 5A / SEMA5A; Recombinant Human Semaphorin 5A / SEMA5A Protein (His tag); FLJ12815; SEMAF; semF; SEMA5A; SEMA5A recombinant protein

• Host: Human Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 1074

• Application Notes: The recombinant human SEMA5A consists of 957 amino acids and predictes a molecular mass of 107 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh SEMA5A is approximately 130-140 kDa due to glycosylation.
• Predicted N Terminal: Glu 23
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for SEMA5A recombinant protein

Background: Semaphorins are secreted, transmembrane, and GPI-linked proteins, defined by cysteine-rich semaphorin protein domains, that have important roles in a variety of tissues. Humans have 20 semaphorins, Drosophila has five, and two are known from DNA viruses. Semaphorins are found in nematodes and crustaceans but not in non-animals. They are grouped into eight classes on the basis of phylogenetic tree analyses and the presence of additional protein motifs. Semaphorins have been implicated in diverse developmental processes such as axon guidance during nervous system development and regulation of cell migration. Semaphorin-5A, also known as Semaphorin-F, Sema F, SEMA5A and SEMAF, is a single-pass type I membrane protein which belongs to the semaphorin family. Semaphorin5A / SEMA5A contains one PSI domain, one Sema domain and seven TSP type -1 domains. It may act as positive axonal guidance cues. Semaphorin5A / SEMA5A is an axon regulator molecule and plays major roles during neuronal and vascular development. It plays an essential role in embryonic development. Semaphorin5A / SEMA5A induces endothelial cell migration from pre-existing vessels. It also plays a role in autism, reducing the ability of neurons to form connections with other neurons in certain brain regions.

Description: A DNA sequence encoding the human SEMA5A (NP_003957.2) extracellular domain (Met 1-Met 968) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 147904700
• NCBI GeneID: 9037
• NCBI Accession #: NP_003957.2
• NCBI GenBank Nucleotide #: NM_003966.2
• UniProt Accession #: Q13591; O60408; Q1RLL9; D3DTC6
• Molecular Weight: 120,615 Da
• NCBI Official Full Name: semaphorin-5A
• NCBI Official Synonym Full Names: semaphorin 5A
• NCBI Official Symbol: SEMA5A
• NCBI Official Synonym Symbols: semF; SEMAF
• NCBI Protein Information: semaphorin-5A
• UniProt Protein Name: Semaphorin-5A
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA5A
• UniProt Synonym Gene Names: SEMAF; Sema F

NCBI Description

This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.[provided by RefSeq, Jan 2010]

Uniprot Description

SEMA5A: a single-pass type I membrane protein that may act as positive axonal guidance cues.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 5p15.31

Cellular Component: integral to membrane; membrane; plasma membrane

Molecular Function: chemorepellent activity; heparan sulfate proteoglycan binding; semaphorin receptor binding; syndecan binding

Biological Process: axonal fasciculation; blood vessel endothelial cell proliferation during sprouting angiogenesis; cell adhesion; cell-cell signaling; diencephalon development; negative regulation of axon extension involved in axon guidance; negative regulation of cell adhesion; nervous system development; neural crest cell migration; positive chemotaxis; positive regulation of actin filament depolymerization; positive regulation of angiogenesis; positive regulation of axon extension involved in axon guidance; positive regulation of cell migration; positive regulation of endothelial cell proliferation; positive regulation of protein kinase B signaling cascade