Semaphorin 5A Recombinant Protein | SEMA5A recombinant protein

Semaphorin 5A Protein, Human, Recombinant (hFc Tag)

Cat. Num. AAA8120779

• Gene Names: SEMA5A; semF; SEMAF
• Purity: >90% as determined by SDS-PAGE
• Synonyms: Semaphorin 5A; Semaphorin 5A Protein; Human; Recombinant (hFc Tag); Human Semaphorin 5A/SEMA5A Protein (Fc Tag); SEMAF Protein; semF Protein; serpin peptidase inhibitor; clade F; SEMA5A recombinant protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Thr765

• Species: Human
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Glu 23
• Tag: C-human IgG1-Fc
• Protein Construction: A DNA sequence encoding the amino acids (Met 1-Thr 765) of human SEMA5A (NP_003957.2) extracellular domain was fused with Fc region of human IgG1 at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for SEMA5A recombinant protein

Background: Semaphorins are secreted, transmembrane, and GPI-linked proteins, defined by cysteine-rich semaphorin protein domains, that have important roles in a variety of tissues. Humans have 2 semaphorins, Drosophila has five, and two are known from DNA viruses. Semaphorins are found in nematodes and crustaceans but not in non-animals. They are grouped into eight classes on the basis of phylogenetic tree analyses and the presence of additional protein motifs. Semaphorins have been implicated in diverse developmental processes such as axon guidance during nervous system development and regulation of cell migration. Semaphorin-5A, also known as Semaphorin-F, Sema F, SEMA5A and SEMAF, is a single-pass type I membrane protein that belongs to the semaphorin family. Semaphorin5A/SEMA5A contains one PSI domain, one Sema domain and seven TSP type-1 domains. It may act as positive axonal guidance cues. Semaphorin5A/SEMA5A is an axon regulator molecule and plays major roles during neuronal and vascular development. It plays an essential role in embryonic development. Semaphorin5A/SEMA5A induces endothelial cell migration from pre-existing vessels. It also plays a role in autism, reducing the ability of neurons to form connections with other neurons in certain brain regions.

References

Strausberg RL. et al., 2003, Proc Natl Acad Sci. 99 (26): 16899-903. Neufeld G. et al., 2005, Front Biosci. 10: 751-60. Fiore R. et al., 2005, Mol Cell Biol. 25 (6): 2310-9. Yazdani U. et al., 2006, Genome Biol. 7 (3): 211. Sadanandam A. et al., 2010, Microvasc Res. 79 (1): 1-9.

NCBI and Uniprot Product Information

• NCBI GI #: 147904700
• NCBI GeneID: 9037
• NCBI Accession #: NP_003957.2
• NCBI GenBank Nucleotide #: NM_003966.2
• UniProt Accession #: Q13591; O60408; Q1RLL9; D3DTC6
• Molecular Weight: 120,615 Da
• NCBI Official Full Name: semaphorin-5A
• NCBI Official Synonym Full Names: semaphorin 5A
• NCBI Official Symbol: SEMA5A
• NCBI Official Synonym Symbols: semF; SEMAF
• NCBI Protein Information: semaphorin-5A
• UniProt Protein Name: Semaphorin-5A
• Protein Family: Semaphorin
• UniProt Gene Name: SEMA5A
• UniProt Synonym Gene Names: SEMAF; Sema F

NCBI Description

This gene belongs to the semaphorin gene family that encodes membrane proteins containing a semaphorin domain and several thrombospondin type-1 repeats. Members of this family are involved in axonal guidance during neural development. This gene has been implicated as an autism susceptibility gene.[provided by RefSeq, Jan 2010]

Uniprot Description

Bifunctional axonal guidance cue regulated by sulfated proteoglycans; attractive effects result from interactions with heparan sulfate proteoglycans (HSPGs), while the inhibitory effects depend on interactions with chondroitin sulfate proteoglycans (CSPGs) (). Ligand for receptor PLXNB3. In glioma cells, SEMA5A stimulation of PLXNB3 results in the disassembly of F-actin stress fibers, disruption of focal adhesions and cellular collapse as well as inhibition of cell migration and invasion through ARHGDIA-mediated inactivation of RAC1. May promote angiogenesis by increasing endothelial cell proliferation and migration and inhibiting apoptosis.