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Western Blot (WB) (Fig. 1: The tissue extract of mouse lung (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CREG1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Fig. 2: The Recombinant Human CREG1 (50ng) protein were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CREG1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Mouse anti-Human CREG1 Monoclonal Antibody | anti-CREG1 antibody

CREG1 antibody

Gene Names
CREG1; CREG
Reactivity
Human
Applications
ELISA, Western Blot
Purity
By protein-A affinity chromatography
Synonyms
CREG1; Monoclonal Antibody; CREG1 antibody; Cellular repressor of E1A-stimulated genes 1; CREG; anti-CREG1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2b,k
Clone Number
AT1C6
Purity/Purification
By protein-A affinity chromatography
Form/Format
Liquid. In phosphate-buffered saline (pH 7.4) with 0.02% Sodium Azide, 10% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
220
Applicable Applications for anti-CREG1 antibody
ELISA (EIA), Western Blot (WB)
Application Notes
Recommended starting dilution is 1:1000.

Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results.
Antigen Species
Human
Immunogen
Recombinant human CREG1 (32-220aa) purified from E. coli
Clone Note
Anti-human CREG1 mAb, clone AT1C6, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CREG1 protein.
Preparation and Storage
Can be stored at 2-8 degree C for 1 week.
For long term storage, aliquot and store at -20 to -80 degree C.
Avoid repeated freezing and thawing cycles.

Western Blot (WB)

(Fig. 1: The tissue extract of mouse lung (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CREG1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Fig. 2: The Recombinant Human CREG1 (50ng) protein were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CREG1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)

Western Blot (WB) (Fig. 1: The tissue extract of mouse lung (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CREG1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Fig. 2: The Recombinant Human CREG1 (50ng) protein were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CREG1 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Related Product Information for anti-CREG1 antibody
CREG1 activity may contribute to the transcriptional control of cell growth and differentiation. Antagonizes transcriptional activation and cellular transformation by the adenovirus E1A protein. CREG1 is broadly expressed in adult tissues and is regulated during embryonic development. CREG1 is a secreted glycoprotein which enhances differentiation of mouse embryonic stem cells and human NTERA-2 cells.
Product Categories/Family for anti-CREG1 antibody
References
Veal E., et al. (1998) Mol Cell Biol 18 (9): 5032-5041; Veal E., et al. (2000) Oncogene 19 (17): 2120-2128

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
protein CREG1
NCBI Official Synonym Full Names
cellular repressor of E1A stimulated genes 1
NCBI Official Symbol
CREG1
NCBI Official Synonym Symbols
CREG
NCBI Protein Information
protein CREG1
UniProt Protein Name
Protein CREG1
Protein Family
UniProt Gene Name
CREG1
UniProt Synonym Gene Names
CREG

NCBI Description

The adenovirus E1A protein both activates and represses gene expression to promote cellular proliferation and inhibit differentiation. The protein encoded by this gene antagonizes transcriptional activation and cellular transformation by E1A. This protein shares limited sequence similarity with E1A and binds both the general transcription factor TBP and the tumor suppressor pRb in vitro. This gene may contribute to the transcriptional control of cell growth and differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

May contribute to the transcriptional control of cell growth and differentiation. Antagonizes transcriptional activation and cellular transformation by the adenovirus E1A protein. The transcriptional control activity of cell growth requires interaction with IGF2R.

Research Articles on CREG1

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Product Notes

The CREG1 creg1 (Catalog #AAA200294) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CREG1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CREG1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Recommended starting dilution is 1:1000. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Researchers should empirically determine the suitability of the CREG1 creg1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CREG1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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