A Disintegrin And Metalloprotease 28 (ADAM28) Recombinant Protein | ADAM28 recombinant protein

Recombinant A Disintegrin And Metalloprotease 28 (ADAM28)

Cat. Num. AAA2010562

• Gene Names: ADAM28; MDCL; MDC-L; ADAM23; MDC-Lm; MDC-Ls; eMDCII; ADAM 28; eMDC II
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation, Immunoprecipitation
• Purity: >98%
• Synonyms: A Disintegrin And Metalloprotease 28 (ADAM28); Recombinant A Disintegrin And Metalloprotease 28 (ADAM28); ADAM28 recombinant protein

• Host: E Coli
• Purity/Purification: >98%
• Form/Format: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-EVADKS CYNRNEGGSKYGYCRRVDDT LIPCKANDTM CGKLFCQGGS DNLPWKGRIV TFLTCKTFDP EDTSQEIGMVANGTKCGDNK VCINAECVDI EKAYKSTNCS SKCKGHAVCD HELQCQCEEG WIPPDCDDSSVVFHFSIVVG VLFPMAVIFV VVAMVIRHQS SREKQKKDQR PLSTTGTRPH KQKRKPQMVKAVQPQEMSQM KPHVYDLPVE GNEPPASFHK DTNALPPTVF KDNPVSTPKD SNPK
• Sequence Length: 775

• Applicable Applications for ADAM28 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP), CoIP, ReporterAssays, Purification, Amine Reactive Labeling.
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Glu525-Lys774 (Accession # Q9UKQ2) with N-terminal His-Tag
• Tissue Specificity: Spleen, Stomach, Intestine, Brain.
• Subcellular Location: Cell membrane; Single-pass type I membrane protein.
• Traits: Freeze-dried powder
• Predicted isoelectric point: 7.5
• Predicted Molecular Mass: 29.3kDa
• Accurate Molecular Mass: 34kDa as determined by SDS-PAGE reducing conditions.
• Phenomenon explanation: The possible reasons that the actual band size differs from the predicted are as follows:; 1. Splice variants: Alternative splicing may create different sized proteins from the same gene.; 2. Relative charge: The composition of amino acids may affects the charge of the protein.; 3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.; 4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.; 5. Polymerization of the target protein: Dimerization, multimerization etc.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 98985828
• NCBI GeneID: 10863
• NCBI Accession #: NP_055080.2
• NCBI GenBank Nucleotide #: NM_014265.4
• UniProt Accession #: Q9UKQ2; Q9Y339; Q9Y3S0; B2RMV5
• NCBI Official Full Name: disintegrin and metalloproteinase domain-containing protein 28 isoform 1 preproprotein
• NCBI Official Synonym Full Names: ADAM metallopeptidase domain 28
• NCBI Official Symbol: ADAM28
• NCBI Official Synonym Symbols: MDCL; MDC-L; ADAM23; MDC-Lm; MDC-Ls; eMDCII; ADAM 28; eMDC II
• NCBI Protein Information: disintegrin and metalloproteinase domain-containing protein 28; metalloproteinase-like, disintegrin-like, and cysteine-rich protein-L; epididymal metalloproteinase-like, disintegrin-like, and cysteine-rich protein II; epididymial metalloproteinase-like, disintegrin-like, and cysteine-rich protein II
• UniProt Protein Name: Disintegrin and metalloproteinase domain-containing protein 28
• Protein Family: Disintegrin and metalloproteinase domain-containing protein
• UniProt Gene Name: ADAM28
• UniProt Synonym Gene Names: ADAM23; MDCL; ADAM 28; eMDC II; MDC-L
• UniProt Entry Name: ADA28_HUMAN

NCBI Description

This gene encodes a member of the ADAM (a disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom disintegrins, and have been implicated in a variety of biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. The protein encoded by this gene is a lymphocyte-expressed ADAM protein. This gene is present in a gene cluster with other members of the ADAM family on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2015]

Uniprot Description

ADAM28: May play a role in the adhesive and proteolytic events that occur during lymphocyte emigration or may function in ectodomain shedding of lymphocyte surface target proteins, such as FASL and CD40L. May be involved in sperm maturation. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; EC 3.4.24.-; Membrane protein, integral; Protease

Chromosomal Location of Human Ortholog: 8p21.2

Cellular Component: integral to membrane; extracellular region; plasma membrane

Molecular Function: metallopeptidase activity; zinc ion binding; metalloendopeptidase activity

Biological Process: spermatogenesis; proteolysis