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Application
Clone
3700 results for "endoplasmic-reticulum-membrane-protein" - showing 2050-2100
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human breast, using Phospho-p53 (S392) Antibody(MBS1750172)TP53 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-TP53 Antibody (MBS1750172)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
p53, Monoclonal Antibody (Cat# AAA1750172)
Full Name
Anti-Phospho-p53 (S392) Rabbit Monoclonal Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
HLA class II histocompatibility antigen, DR alpha chain (HLA-DRA), Recombinant Protein (Cat# AAA1265308)
Full Name
Recombinant Human HLA class II histocompatibility antigen, DR alpha chain (HLA-DRA), partial
Gene Names
HLA-DRA; MLRW; HLA-DRA1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Pricing
$1,415/1 mg (E-Coli) | $395/0.1 mg (E-Coli) | $245/0.02 mg (E-Coli) | $6,205/5x1 mg (E-Coli)
Stress-associated endoplasmic reticulum protein 1 (SERP1), Recombinant Protein (Cat# AAA7057018)
Full Name
Recombinant Pongo abelii Stress-associated endoplasmic reticulum protein 1 (SERP1), partial
Gene Names
SERP1; RAMP4
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Western Blot (WB)
(Western blot analysis of Rat Pancreas cell lysates showing detection of 46.2kDa VMP1 protein using Rabbit Anti-VMP1 Polyclonal Antibody (SPC-680). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody (SPC-680) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 46.2kDa. Other Band(s): Multiple bands for A549; Rat Brain shows a band at 60kDa, 75kDa, and 150kDa.)
VMP1, Polyclonal Antibody (Cat# AAA809994)
Full Name
VMP1 Antibody
Gene Names
VMP1; EPG3; TANGO5; TMEM49
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$430/0.1 mg | $1,695/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Cyclophilin B expression in HepG2 cell lysate (MBS179945).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PPIB monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PPIB )
Cyclophilin B, Monoclonal Antibody (Cat# AAA179945)
Full Name
Anti-Cyclophilin B Rabbit Monoclonal Antibody
Gene Names
PPIB; OI9; CYPB; SCYLP; CYP-S1; HEL-S-39
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 3D7 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Cells were subject to heat shock at 42 degree C for 2 hours and transferred to CO2 incubator at 37 degree C for 1 hour before trypsinization.)
CD74, Monoclonal Antibody (Cat# AAA808467)
Full Name
CD74 Antibody, Clone 3D7: ATTO 390
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of Bcl-2 using anti-Bcl-2 antibody (MBS1750313).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: rat liver tissue lysate,Lane 2: mouse thymus tissue lysate,Lane 3: human MCF-7 whole Cell lysate,Lane 4: human 22RV1 whole Cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Bcl-2 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Bcl-2 at approximately 29KD. The expected band size for Bcl-2 is at 26KD. )
Bcl-2, Polyclonal Antibody (Cat# AAA1750313)
Full Name
Anti-Bcl-2 Picoband Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse, Rat
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
FC/FACS, IHC, ICC, WB
Purity
Immunogen affinity purified
Pricing
$345/0.1 mg | $1,485/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Phospho-EGFR (Y1086) expression in (1) A431 cell lysate; (2) A431 cell lysate treated with EGF (MBS1750184).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EGFR )
EGFR, Monoclonal Antibody (Cat# AAA1750184)
Full Name
Anti-Phospho-EGFR (Y1086) Rabbit Monoclonal Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of mTOR expression in Jurkat cell lysate (MBS179041).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MTOR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MTOR )
mTOR/Tor, Monoclonal Antibody (Cat# AAA179041)
Full Name
Anti-mTOR/Tor Rabbit Monoclonal Antibody
Gene Names
MTOR; SKS; FRAP; FRAP1; FRAP2; RAFT1; RAPT1
Reactivity
Human, Mouse, Rat
Applications
FC/FACS
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Mouse Kidney, Liver showing detection of ~52.7 kDa Presenilin 1 protein using Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-755). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver. Lane 3: Mouse Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-755) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~52.7 kDa. Other Band(s): 42.7 kDa band is isoform 5 .)
Presenilin 1, Polyclonal Antibody (Cat# AAA8001030)
Full Name
Presenilin 1 Antibody: Alkaline Phosphatase
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western blot analysis of perk in lysates of HepG2 , using perk Antibody(AF4799).)
PERK, Polyclonal Antibody (Cat# AAA9613506)
Full Name
PERK Antibody
Gene Names
EIF2AK3; PEK; WRS; PERK
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Rabbit (100%), Dog (100%), Xenopus (82%)
Predicted Reactivity: Rabbit (100%), Dog (100%), Xenopus (82%)
Applications
WB, EIA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin
Pricing
$245/0.05 mL | $305/0.1 mL | $365/0.2 mL | $1,495/5x0.2 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 1B8 . Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell membrane, Endoplasmic Reticulum, Golgi apparatus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) CD74 Antibody (D) Composite.)
CD74, Monoclonal Antibody (Cat# AAA808449)
Full Name
CD74 Antibody, Clone 1B8: ATTO 390
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human
Applications
WB, ICC, IF
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 3D7 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Cells were subject to heat shock at 42 degree C for 2 hours and transferred to CO2 incubator at 37 degree C for 1 hour before trypsinization.)
CD74, Monoclonal Antibody (Cat# AAA808482)
Full Name
CD74 Antibody, Clone 3D7: RPE
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 3D7 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Cells were subject to heat shock at 42 degree C for 2 hours and transferred to CO2 incubator at 37 degree C for 1 hour before trypsinization.)
CD74, Monoclonal Antibody (Cat# AAA808466)
Full Name
CD74 Antibody, Clone 3D7
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 3D7 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Cells were subject to heat shock at 42 degree C for 2 hours and transferred to CO2 incubator at 37 degree C for 1 hour before trypsinization.)
CD74, Monoclonal Antibody (Cat# AAA808479)
Full Name
CD74 Antibody, Clone 3D7: HRP
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$465/0.1 mg | $1,875/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse, Rat liver lysate showing detection of ~52.7 kDa Presenilin 1 protein using Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-734). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse liver lysate. Lane 3: Rat liver lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-734) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~52.7 kDa. Other Band(s): 28, 32, 42 kDa other isoforms.)
Presenilin 1, Polyclonal Antibody (Cat# AAA8000645)
Full Name
Presenilin 1 Antibody: ATTO 488
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3
Reactivity
Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 14 kDa GABARAPL1 protein using Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 14 kDa.)
GABARAPL1, Polyclonal Antibody (Cat# AAA809474)
Full Name
GABARAPL1 Antibody: ATTO 488
Gene Names
GABARAPL1; ATG8; GEC1; APG8L; ATG8B; ATG8L; APG8-LIKE
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Figure 1. Western blot analysis of PREB using anti-PREB antibody (MBS1750886).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: human Hela whole cell lysate,Lane 2: human placenta tissue lysate,Lane 3: human A549 whole cell lysate.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PREB antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PREB at approximately 45KD. The expected band size for PREB is at 45KD. )
PREB, Polyclonal Antibody (Cat# AAA1750886)
Full Name
Anti-PREB Picoband Antibody
Gene Names
PREB; SEC12
Reactivity
Human
No cross reactivity with other proteins.
No cross reactivity with other proteins.
Applications
WB
Purity
Immunogen affinity purified
Pricing
$310/0.1 mg | $1,330/5x0.1 mg
Western Blot (WB)
(Western blot analysis of KCNA1 expression in Human fetal brain lysate (MBS179740).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KCNA1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for KCNA1 )
KCNA1/Kv1.1, Monoclonal Antibody (Cat# AAA179740)
Full Name
Anti-KCNA1/Kv1.1 Rabbit Monoclonal Antibody
Gene Names
KCNA1; EA1; MK1; AEMK; HBK1; HUK1; MBK1; RBK1; KV1.1
Reactivity
Human
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human spleen, using Bcl-2 Antibody(MBS179053)BCL2 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-BCL2 Antibody (MBS179053)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
Bcl-2, Monoclonal Antibody (Cat# AAA179053)
Full Name
Anti-Bcl-2 Rabbit Monoclonal Antibody
Gene Names
BCL2; Bcl-2; PPP1R50
Reactivity
Human, Mouse
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunohistochemistry (IHC)
(Immunohistochemical analysis of paraffin-embedded human pancreas, using GRP78 BiP Antibody(MBS179500)HSPA5 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-HSPA5 Antibody (MBS179500)overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen. )
GRP78 BiP, Monoclonal Antibody (Cat# AAA179500)
Full Name
Anti-GRP78 BiP Rabbit Monoclonal Antibody
Gene Names
HSPA5; BIP; MIF2; GRP78; HEL-S-89n
Reactivity
Human, Mouse, Rat
Applications
IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
ELOVL1 / Elongation Of Very Long Chain Fatty Acids Protein 1, Recombinant Protein (Cat# AAA2902483)
Full Name
Human ELOVL1 / Elongation Of Very Long Chain Fatty Acids Protein 1 Recombinant Protein
Gene Names
ELOVL1; Ssc1; IKSHD; CGI-88
Applications
EIA, WB, AP
Purity
>95% by SDS-PAGE
Western Blot (WB)
(All lanes : Anti-TAP2 Antibody (N-Term) at 1:2000 dilutionLane 1: Human placenta lysateLane 2: Jurkat whole cell lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 76 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
TAP2, Polyclonal Antibody (Cat# AAA9231245)
Full Name
TAP2 Antibody (N-Term)
Gene Names
TAP2; APT2; PSF2; ABC18; ABCB3; PSF-2; RING11; D6S217E
Reactivity
Human
Applications
WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Mouse Kidney, Liver showing detection of ~52.7 kDa Presenilin 1 protein using Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-755). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver. Lane 3: Mouse Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-755) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~52.7 kDa. Other Band(s): 42.7 kDa band is isoform 5 .)
Presenilin 1, Polyclonal Antibody (Cat# AAA8001022)
Full Name
Presenilin 1 Antibody: ATTO 390
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of PDI expression in HeLa cell lysate (MBS179949).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDIA2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDIA2 )
PDI, Monoclonal Antibody (Cat# AAA179949)
Full Name
Anti-PDI Rabbit Monoclonal Antibody
Gene Names
PDIA2; PDI; PDA2; PDIP; PDIR
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 14 kDa GABARAPL1 protein using Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 14 kDa.)
GABARAPL1, Polyclonal Antibody (Cat# AAA809487)
Full Name
GABARAPL1 Antibody: PerCP
Gene Names
GABARAPL1; ATG8; GEC1; APG8L; ATG8B; ATG8L; APG8-LIKE
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~134.3 kDa EGFR protein using Rabbit Anti-EGFR Polyclonal Antibody (SPC-771). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-EGFR Polyclonal Antibody (SPC-771) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~134.3 kDa. Other Band(s): 44 kDa and 69 kDa.)
EGFR, Polyclonal Antibody (Cat# AAA8001325)
Full Name
EGFR Antibody: RPE
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western blot analysis of AGR2 expression in MCF-7 cell lysate (MBS179913).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-AGR2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for AGR2 )
AGR2/Ag 2, Monoclonal Antibody (Cat# AAA179913)
Full Name
Anti-AGR2/Ag 2 Rabbit Monoclonal Antibody
Gene Names
AGR2; AG2; AG-2; HPC8; GOB-4; HAG-2; XAG-2; PDIA17; HEL-S-116
Reactivity
Human
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho-EGFR (S695) expression in A431 cell lysate treated with EGF (MBS1750187).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGFR monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EGFR )
EGFR, Monoclonal Antibody (Cat# AAA1750187)
Full Name
Anti-Phospho-EGFR (S695) Rabbit Monoclonal Antibody
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of PGP95 expression in 293T cell lysate (MBS179524).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-UCHL1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for UCHL1 )
PGP9.5, Monoclonal Antibody (Cat# AAA179524)
Full Name
Anti-PGP9.5 Rabbit Monoclonal Antibody
Gene Names
UCHL1; NDGOA; PARK5; PGP95; SPG79; PGP9.5; Uch-L1; HEL-117; PGP 9.5; HEL-S-53
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Phospho-p53 (S376) in HEK293 cell lysates treated with Calyculin A and Okadaic Acid (MBS1750173).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TP53 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TP53 )
p53, Monoclonal Antibody (Cat# AAA1750173)
Full Name
Anti-Phospho-p53 (S376) Rabbit Monoclonal Antibody
Gene Names
TP53; P53; BCC7; LFS1; TRP53
Reactivity
Human
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of LRRK2 in HEK293 cell lysate transfected with 3*DYKDDDDK wild type, full length LRRK2 (MBS179199).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LRRK2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LRRK2 )
LRRK2, Monoclonal Antibody (Cat# AAA179199)
Full Name
Anti-LRRK2 Rabbit Monoclonal Antibody
Gene Names
LRRK2; PARK8; RIPK7; ROCO2; AURA17; DARDARIN
Reactivity
Human, Mouse, Rat
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Stress-associated endoplasmic reticulum protein 1 (Serp1), Recombinant Protein (Cat# AAA7057017)
Full Name
Recombinant Mouse Stress-associated endoplasmic reticulum protein 1 (Serp1), partial
Gene Names
Serp1; Ramp4; D3Ucla1
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
BELCIN 1, Polyclonal Antibody (Cat# AAA8554164)
Full Name
BELCIN 1 Rabbit pAb
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human
Applications
DB
Purity
Affinity Purified
Pricing
$310/0.1 mL | $570/0.1 mL (Biotin) | $1,330/5x0.1 mL | $570/0.1 mL (FITC) | $570/0.1 mL (AF350) | $570/0.1 mL (AF405) | $570/0.1 mL (AF488) | $570/0.1 mL (AF555) | $570/0.1 mL (AF568)
Western Blot (WB)
(Western blot analysis of Rat Pancreas cell lysates showing detection of 46.2kDa VMP1 protein using Rabbit Anti-VMP1 Polyclonal Antibody (SPC-680). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody (SPC-680) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 46.2kDa. Other Band(s): Multiple bands for A549; Rat Brain shows a band at 60kDa, 75kDa, and 150kDa.)
VMP1, Polyclonal Antibody (Cat# AAA8000010)
Full Name
VMP1 Antibody: PerCP
Gene Names
VMP1; EPG3; TANGO5; TMEM49
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Transmembrane protein 173 (TMEM173) Standard Curve.)
Transmembrane Protein 173 (TMEM173), ELISA Kit (Cat# AAA9713949)
Full Name
Human Transmembrane Protein 173 (TMEM173) ELISA Kit
Gene Names
TMEM173; ERIS; MITA; MPYS; SAVI; NET23; STING; hMITA; hSTING; STING-beta
Reactivity
Human
Pricing
$490/96 Tests | $330/48 Tests | $1,810/5x96 Tests
Western Blot (WB)
(Western blot analysis of Rat Pancreas cell lysates showing detection of 46.2kDa VMP1 protein using Rabbit Anti-VMP1 Polyclonal Antibody (SPC-680). Lane 1: Molecular Weight Ladder (MW). Lane 2: Rat Pancreas cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-VMP1 Polyclonal Antibody (SPC-680) at 1:1000 for 16 hours at 4 degree C. Secondary Antibody: Goat-Anti-Rabbit IgG: HRP at 1:200 for 60 min at RT. Color Development: TMB. Predicted/Observed Size: 46.2kDa. Other Band(s): Multiple bands for A549; Rat Brain shows a band at 60kDa, 75kDa, and 150kDa.)
VMP1, Polyclonal Antibody (Cat# AAA809995)
Full Name
VMP1 Antibody: ATTO 390
Gene Names
VMP1; EPG3; TANGO5; TMEM49
Reactivity
Human
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,940/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse, Rat liver lysate showing detection of ~52.7 kDa Presenilin 1 protein using Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-734). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse liver lysate. Lane 3: Rat liver lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-734) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~52.7 kDa. Other Band(s): 28, 32, 42 kDa other isoforms.)
Presenilin 1, Polyclonal Antibody (Cat# AAA8000652)
Full Name
Presenilin 1 Antibody: Alkaline Phosphatase
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3
Reactivity
Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,905/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Kidney, Liver showing detection of ~52.7 kDa Presenilin 1 protein using Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-755). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver. Lane 3: Mouse Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-755) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~52.7 kDa. Other Band(s): 42.7 kDa band is isoform 5 .)
Presenilin 1, Polyclonal Antibody (Cat# AAA8001023)
Full Name
Presenilin 1 Antibody: ATTO 488
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse, Rat liver lysate showing detection of ~52.7 kDa Presenilin 1 protein using Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-734). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse liver lysate. Lane 3: Rat liver lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-734) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~52.7 kDa. Other Band(s): 28, 32, 42 kDa other isoforms.)
Presenilin 1, Polyclonal Antibody (Cat# AAA8000655)
Full Name
Presenilin 1 Antibody: FITC
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3
Reactivity
Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$470/0.1 mg | $1,895/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse Kidney, Liver showing detection of ~52.7 kDa Presenilin 1 protein using Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-755). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse Liver. Lane 3: Mouse Kidney. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-755) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~52.7 kDa. Other Band(s): 42.7 kDa band is isoform 5 .)
Presenilin 1, Polyclonal Antibody (Cat# AAA8001032)
Full Name
Presenilin 1 Antibody: Biotin
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Mouse, Rat liver lysate showing detection of ~52.7 kDa Presenilin 1 protein using Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-734). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse liver lysate. Lane 3: Rat liver lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-734) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~52.7 kDa. Other Band(s): 28, 32, 42 kDa other isoforms.)
Presenilin 1, Polyclonal Antibody (Cat# AAA8000659)
Full Name
Presenilin 1 Antibody: RPE
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3
Reactivity
Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 14 kDa GABARAPL1 protein using Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 14 kDa.)
GABARAPL1, Polyclonal Antibody (Cat# AAA809485)
Full Name
GABARAPL1 Antibody: HRP
Gene Names
GABARAPL1; ATG8; GEC1; APG8L; ATG8B; ATG8L; APG8-LIKE
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 1B8 . Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell membrane, Endoplasmic Reticulum, Golgi apparatus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) CD74 Antibody (D) Composite.)
CD74, Monoclonal Antibody (Cat# AAA808448)
Full Name
CD74 Antibody, Clone 1B8
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human
Applications
WB, ICC, IF
Purity
Protein G Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB)
(Western blot analysis of FGFR4 expression in Raji cell lysate (MBS179446).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR4 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FGFR4 )
FGFR4, Monoclonal Antibody (Cat# AAA179446)
Full Name
Anti-FGFR4 Rabbit Monoclonal Antibody
Gene Names
FGFR4; TKF; JTK2; CD334
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 6D9 . Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell membrane, Endoplasmic Reticulum, Golgi apparatus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) CD74 Antibody (D) Composite.)
CD74, Monoclonal Antibody (Cat# AAA808499)
Full Name
CD74 Antibody, Clone 6D9: PerCP
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human
Applications
WB, ICC, IF
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,925/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human HeLa and HEK293Trap cell lysates showing detection of 14 kDa GABARAPL1 protein using Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622). Lane 1: Molecular Weight Ladder (MW). Lane 2: Human HeLa and 293Trap cell lysates. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-GABARAPL1 Polyclonal Antibody (SPC-622) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 6 min in RT. Predicted/Observed Size: 14 kDa.)
GABARAPL1, Polyclonal Antibody (Cat# AAA809476)
Full Name
GABARAPL1 Antibody: ATTO 594
Gene Names
GABARAPL1; ATG8; GEC1; APG8L; ATG8B; ATG8L; APG8-LIKE
Reactivity
Human; Mouse
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 6D9 . Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell membrane, Endoplasmic Reticulum, Golgi apparatus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) CD74 Antibody (D) Composite.)
CD74, Monoclonal Antibody (Cat# AAA808495)
Full Name
CD74 Antibody, Clone 6D9: Biotin
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human
Applications
WB, ICC, IF
Purity
Protein G Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~134.3 kDa EGFR protein using Rabbit Anti-EGFR Polyclonal Antibody (SPC-772). Lane 1: Molecular Weight Ladder (MW). Lane 2: HeLa. Load: 10 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-EGFR Polyclonal Antibody (SPC-772) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit IgG: HRP at 1:5000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~134.3 kDa. Other Band(s): 44 kDa and 69 kDa.)
EGFR, Polyclonal Antibody (Cat# AAA8001331)
Full Name
EGFR Antibody: ATTO 594
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
Reactivity
Human
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of FGFR1 expression in SH-SY5Y cell lysate (MBS179100).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FGFR1 )
FGFR1, Monoclonal Antibody (Cat# AAA179100)
Full Name
Anti-FGFR1 Rabbit Monoclonal Antibody
Gene Names
FGFR1; CEK; FLG; HH2; OGD; ECCL; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; HRTFDS; bFGF-R-1
Reactivity
Human
Applications
IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
