Rabbit anti-Human RPS6 Polyclonal Antibody | anti-RPS6 antibody

RPS6, NT (40S Ribosomal Protein S6, Phosphoprotein NP33, OK/SW-cl.2) (Azide free) (HRP)

Cat. Num. AAA6497667

• Gene Names: RPS6; S6
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RPS6; Polyclonal Antibody; NT (40S Ribosomal Protein S6; Phosphoprotein NP33; OK/SW-cl.2) (Azide free) (HRP); anti-RPS6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RPS6.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-RPS6 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:50; ELISA: 1:1,000; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the N-terminal region of human RPS6 (KLH).
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of RPS6 Antibody (N-term) in HL60 cell line lysates (35ug/lane). RPS6 (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of RPS6 (arrow) using rabbit polyclonal RPS6 Antibody (N-term) (RB12889). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the RPS6 gene (Lane 2) (Origene Technologies).)

Product Categories/Family for anti-RPS6 antibody

Antibodies; Ribosomal Proteins

References

1. Lott J.B., Gene 65:31-39(1988). 2. Heinze H., J. Biol. Chem. 263:4139-4144(1988). 3. Antoine M., Hum. Mol. Genet. 1:565-570(1992). 4. Pata I., Gene 121:387-392(1992).

NCBI and Uniprot Product Information

• NCBI GI #: 17158044
• NCBI GeneID: 6194
• NCBI Accession #: NP_001001
• NCBI GenBank Nucleotide #: NM_001010
• UniProt Accession #: P62753
• Molecular Weight: 28,681 Da
• NCBI Official Full Name: 40S ribosomal protein S6
• NCBI Official Synonym Full Names: ribosomal protein S6
• NCBI Official Symbol: RPS6
• NCBI Official Synonym Symbols: S6
• NCBI Protein Information: 40S ribosomal protein S6; phosphoprotein NP33
• UniProt Protein Name: 40S ribosomal protein S6
• Protein Family: 30S ribosomal protein
• UniProt Gene Name: RPS6
• UniProt Entry Name: RS6_HUMAN

Uniprot Description

S6: May play an important role in controlling cell growth and proliferation through the selective translation of particular classes of mRNA. Belongs to the ribosomal protein S6e family.

Protein type: Translation

Chromosomal Location of Human Ortholog: 9p21

Cellular Component: polysome; small ribosomal subunit; membrane; perinuclear region of cytoplasm; dendrite; cytoplasm; nucleolus; nucleus; ribonucleoprotein complex; cytosol

Molecular Function: protein binding; structural constituent of ribosome; protein kinase binding

Biological Process: SRP-dependent cotranslational protein targeting to membrane; erythrocyte development; TOR signaling pathway; translation; viral reproduction; positive regulation of apoptosis; T cell differentiation in the thymus; gastrulation; translational termination; viral infectious cycle; glucose homeostasis; oogenesis stage; T cell proliferation during immune response; translational initiation; ribosomal small subunit assembly and maintenance; viral transcription; activated T cell apoptosis; placenta development; mitosis; mitotic cell cycle checkpoint; ribosomal small subunit biogenesis and assembly; translational elongation; cellular protein metabolic process; mRNA catabolic process, nonsense-mediated decay; insulin receptor signaling pathway; gene expression; rRNA processing; G1/S transition of mitotic cell cycle; negative regulation of apoptosis