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Type
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Gene Name
Isotype
Host
Application
Clone
3330 results for "golgi-apparatus-protein" - showing 1750-1800
GLG1, siRNA (Cat# AAA8218212)
Full Name
GLG1 siRNA (Mouse)
Gene Names
Glg1; CFR; CFR-1; ESL-1; MG160; Selel; MG-160; AI593353; AW537898
Reactivity
Mouse
Applications
RNAi
Purity
> 97%
Pricing
$395/15 nmol | $555/30 nmol | $2,440/5x30 nmol
Flow Cytometry (FC/FACS)
(Flow Cytometry analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 3D7 . Tissue: Neuroblastoma cells (SH-SY5Y). Species: Human. Fixation: 90% Methanol. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 30 min on ice. Secondary Antibody: Goat Anti-Mouse: PE at 1:100 for 20 min at RT. Cells were subject to heat shock at 42 degree C for 2 hours and transferred to CO2 incubator at 37 degree C for 1 hour before trypsinization.)
CD74, Monoclonal Antibody (Cat# AAA808468)
Full Name
CD74 Antibody, Clone 3D7: ATTO 488
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human
Applications
WB, FC/FACS
Purity
Protein G Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
Western Blot (WB)
(Western blot analysis of MHC Class II expression in Daudi cell lysate (MBS179339).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HLA-DPB1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HLA-DPB1 )
MHC Class II, Monoclonal Antibody (Cat# AAA179339)
Full Name
Anti-MHC Class II Rabbit Monoclonal Antibody
Gene Names
HLA-DPB1; DPB1; HLA-DP; HLA-DPB; HLA-DP1B
Reactivity
Human
Applications
IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Golgi apparatus membrane protein tvp23 (tvp23), Recombinant Protein (Cat# AAA7063630)
Full Name
Recombinant Aspergillus niger Golgi apparatus membrane protein tvp23 (tvp23), partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Golgi apparatus membrane protein tvp38 (tvp38), Recombinant Protein (Cat# AAA7071161)
Full Name
Recombinant Neosartorya fumigata Golgi apparatus membrane protein tvp38 (tvp38), partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Golgi apparatus membrane protein TVP38 (TVP38), Recombinant Protein (Cat# AAA7065180)
Full Name
Recombinant Saccharomyces cerevisiae Golgi apparatus membrane protein TVP38 (TVP38), partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Golgi apparatus membrane protein tvp18 (tvp18), Recombinant Protein (Cat# AAA7070933)
Full Name
Recombinant Schizosaccharomyces pombe Golgi apparatus membrane protein tvp18 (tvp18), partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Golgi apparatus membrane protein TVP18 (TVP18), Recombinant Protein (Cat# AAA7064307)
Full Name
Recombinant Magnaporthe oryzae Golgi apparatus membrane protein TVP18 (TVP18), partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
TMEM5, Blocking Peptide (Cat# AAA9221942)
Full Name
TMEM5 Antibody (N-term) Blocking Peptide
Gene Names
TMEM5; HP10481; MDDGA10
GAD2, Blocking Peptide (Cat# AAA9218151)
Full Name
GAD2 Antibody (Center) Blocking peptide
Gene Names
GAD2; GAD65
CSGALNACT1, Blocking Peptide (Cat# AAA9225426)
Full Name
CSGALNACT1 Antibody (Center) Blocking Peptide
Gene Names
CSGALNACT1; ChGn; CSGalNAcT-1; beta4GalNAcT
Western Blot (WB)
GABARAP, Polyclonal Antibody (Cat# AAA807659)
Full Name
Rabbit Anti-Human GABARAP Polyclonal
Gene Names
GABARAP; MM46; ATG8A; GABARAP-a
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,915/5x0.1 mg
Western Blot (WB)
GABARAP, Polyclonal Antibody (Cat# AAA807422)
Full Name
Rabbit Anti-Human GABARAP Polyclonal
Gene Names
GABARAP; MM46; ATG8A; GABARAP-a
Reactivity
Mouse
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,930/5x0.1 mg
CHST4, Blocking Peptide (Cat# AAA9229924)
Full Name
CHST4 Blocking Peptide (C-term)
Gene Names
CHST4; GST3; LSST; GlcNAc6ST2; HECGLCNAC6ST
PKD2, Blocking Peptide (Cat# AAA9227392)
Full Name
PKD2 Antibody (C-term) Blocking Peptide
Gene Names
PRKD2; PKD2; HSPC187; nPKC-D2
EGFR (Y998), Blocking Peptide (Cat# AAA9225117)
Full Name
Phospho-EGFR(Y998) Antibody Blocking peptide
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
FGFR (Y766), Blocking Peptide (Cat# AAA9225020)
Full Name
Phospho-FGFR(Y766) Antibody Blocking peptide
Gene Names
FGFR1; CEK; FLG; HH2; OGD; ECCL; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; HRTFDS; bFGF-R-1
Western Blot (WB)
(Western blot analysis of Caveolin 3 expression in Human fetal heart lysate (MBS179512).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CAV3 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CAV3 )
Caveolin-3, Monoclonal Antibody (Cat# AAA179512)
Full Name
Anti-Caveolin-3 Rabbit Monoclonal Antibody
Gene Names
CAV3; LQT9; VIP21; LGMD1C; VIP-21
Reactivity
Human, Mouse, Rat
Applications
WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(All lanes : Anti-NSFL1C Antibody (Center) at 1:2000 dilutionLane 1: 293 whole cell lysateLane 2: A431 whole cell lysateLane 3: NCI-H1299 whole cell lysateLane 4: U-251 MG whole cell lysateLane 5: Mouse brain lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 41 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
NSFL1C, Polyclonal Antibody (Cat# AAA9231042)
Full Name
NSFL1C Antibody (Center)
Gene Names
NSFL1C; P47; UBX1; UBXD10; UBXN2C; dJ776F14.1
Reactivity
Human, Mouse
Predicted: Bovine, Rat
Predicted: Bovine, Rat
Applications
WB, EIA
Pricing
$185/0.05 mL | $415/0.2 mL | $1,625/5x0.2 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-GABARAPL2 Polyclonal Antibody . Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Rabbit Anti-GABARAPL2 Polyclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Rabbit ATTO 488 at 1:100 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasmic Vesicle, Autophagosome . Magnification: 60X. (A) DAPI nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) GABARAPL2 Antibody. (D) Composite.)
GABARAPL2, Polyclonal Antibody (Cat# AAA809494)
Full Name
GABARAPL2 Antibody: ATTO 594
Gene Names
GABARAPL2; ATG8; GEF2; ATG8C; GEF-2; GATE16; GATE-16
Reactivity
Human; Rat
Applications
WB, ICC, IF
Purity
Peptide Affinity Purified
Pricing
$480/0.1 mg | $1,935/5x0.1 mg
Western Blot (WB)
(Western blot analysis of FGFR2 expression in (1) MCF-7 cell lysate; (2) Mouse brain lysate (MBS179209).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FGFR2 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FGFR2 )
FGFR2, Monoclonal Antibody (Cat# AAA179209)
Full Name
Anti-FGFR2 Rabbit Monoclonal Antibody
Gene Names
FGFR2; BEK; JWS; BBDS; CEK3; CFD1; ECT1; KGFR; TK14; TK25; BFR-1; CD332; K-SAM
Reactivity
Human, Mouse, Rat
Applications
IP, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Protein ECHIDNA (ECH), Recombinant Protein (Cat# AAA7096153)
Full Name
Recombinant Arabidopsis thaliana Protein ECHIDNA (ECH), partial
Gene Names
AT1G09330; ECH; ECHIDNA; T31J12.5; T31J12_5
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Golgi apparatus membrane protein TVP23 (TVP23), Recombinant Protein (Cat# AAA7066992)
Full Name
Recombinant Podospora anserina Golgi apparatus membrane protein TVP23 (TVP23), partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Golgi apparatus membrane protein tvp23 (tvp23), Recombinant Protein (Cat# AAA7063256)
Full Name
Recombinant Aspergillus clavatus Golgi apparatus membrane protein tvp23 (tvp23), partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
SEC24C, Blocking Peptide (Cat# AAA9225361)
Full Name
SEC24C Antibody (Center) Blocking Peptide
STX10, Blocking Peptide (Cat# AAA9228485)
Full Name
STX10 Antibody (N-term) Blocking Peptide
Gene Names
STX10; SYN10; hsyn10
RAB26, Blocking Peptide (Cat# AAA9222709)
Full Name
RAB26 Antibody (C-term) Blocking Peptide
Gene Names
RAB26; V46133
CSGALNACT2, Blocking Peptide (Cat# AAA9227842)
Full Name
CSGALNACT2 Antibody (Center) Blocking Peptide
ST3GAL4, Blocking Peptide (Cat# AAA9230617)
Full Name
ST3GAL4 Blocking Peptide (N-Term)
Gene Names
ST3GAL4; STZ; SAT3; CGS23; SIAT4; NANTA3; SIAT4C; ST3GalIV
PCSK7, Blocking Peptide (Cat# AAA9220409)
Full Name
PCSK7 Antibody (C-term) Blocking peptide
Gene Names
PCSK7; LPC; PC7; PC8; SPC7
RAB13, Blocking Peptide (Cat# AAA9226241)
Full Name
RAB13 Antibody (Center) Blocking Peptide
Gene Names
RAB13; GIG4
EGFR (Y1092), Blocking Peptide (Cat# AAA9225123)
Full Name
Phospho-EGFR(Y1092) Antibody Blocking peptide
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
EGFR (Y869), Blocking Peptide (Cat# AAA9227077)
Full Name
EGFR Antibody (Y869) Blocking Peptide
Gene Names
EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2
LLGL1, Blocking Peptide (Cat# AAA9231201)
Full Name
LLGL1 Blocking Peptide (N-term)
Gene Names
LLGL1; DLG4; HUGL; LLGL; Lgl1; Mgl1; HUGL1; HUGL-1
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-600). Lane 1: MW Ladder. Lane 2: Human HeLa (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-600) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa.)
Beclin 1, Polyclonal Antibody (Cat# AAA807395)
Full Name
Rabbit Anti-Human Beclin 1 Polyclonal
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human, Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$465/0.1 mg | $1,880/5x0.1 mg
Western Blot (WB)
(Anti-MPL Antibody (C-Term) at 1:2000 dilution + Mouse spleen lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 70 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
MPL, Polyclonal Antibody (Cat# AAA9231253)
Full Name
MPL Antibody (C-Term)
Gene Names
MPL; MPLV; TPOR; C-MPL; CD110; THCYT2
Reactivity
Mouse
Predicted: Human
Predicted: Human
Applications
WB, EIA
Purity
Purified through a protein A column, followed by peptide affinity purification.
Pricing
$185/0.05 mL | $405/0.2 mL | $1,585/5x0.2 mL
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-601). Lane 1: MW Ladder. Lane 2: Human HeLa (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-601) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa.)
Beclin 1, Polyclonal Antibody (Cat# AAA807651)
Full Name
Rabbit Anti-Human Beclin 1 Polyclonal
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human, Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,920/5x0.1 mg
Western Blot (WB)
(Western blot analysis of ROCK1 expression in Ramos cell lysate (MBS179425).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ROCK1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ROCK1 )
ROCK1, Monoclonal Antibody (Cat# AAA179425)
Full Name
Anti-ROCK1 Rabbit Monoclonal Antibody
Gene Names
ROCK1; ROCK-I; P160ROCK
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IP, IF, IHC, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Uncharacterized FAM18-like protein 2 (DDB_G0286703), Recombinant Protein (Cat# AAA7082026)
Full Name
Recombinant Dictyostelium discoideum Uncharacterized FAM18-like protein 2 (DDB_G0286703), partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Golgi apparatus membrane protein TVP23 (TVP23), Recombinant Protein (Cat# AAA7072825)
Full Name
Recombinant Phaeosphaeria nodorum Golgi apparatus membrane protein TVP23 (TVP23), partial
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
PITPNB, Blocking Peptide (Cat# AAA9219560)
Full Name
PITPNB Antibody (C-term) Blocking peptide
Gene Names
PITPNB; VIB1B; PtdInsTP; PI-TP-beta
UNC50, Blocking Peptide (Cat# AAA9225605)
Full Name
UNC50 Antibody (N-term) Blocking peptide
Gene Names
UNC50; URP; GMH1; UNCL; HSD23
CHST1, Blocking Peptide (Cat# AAA9229850)
Full Name
CHST1 Blocking Peptide (C-term)
Gene Names
CHST1; C6ST; KSST; GST-1; KS6ST; KSGal6ST
CHPT1, Blocking Peptide (Cat# AAA9217468)
Full Name
CHPT1 Antibody (N-term) Blocking peptide
Gene Names
CHPT1; CPT; CPT1
TRPM4, Blocking Peptide (Cat# AAA9221404)
Full Name
TRPM4 Antibody (C-term) Blocking Peptide
Gene Names
TRPM4; LTrpC4; PFHB1B; TRPM4B; hTRPM4
Western Blot (WB)
(Western blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of ~51kDa Beclin 1 protein using Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-600). Lane 1: MW Ladder. Lane 2: Human HeLa (20 ug). Load: 20 ug. Block: 5% milk + TBST for 1 hour at RT. Primary Antibody: Rabbit Anti-Beclin 1 Polyclonal Antibody (SPC-600) at 1:1000 for 1 hour at RT. Secondary Antibody: Goat Anti-Rabbit: HRP at 1:2000 for 1 hour at RT. Color Development: TMB solution for 12 min at RT. Predicted/Observed Size: ~51kDa.)
Beclin 1, Polyclonal Antibody (Cat# AAA807549)
Full Name
Rabbit Anti-Human Beclin 1 Polyclonal
Gene Names
BECN1; ATG6; VPS30; beclin1
Reactivity
Human, Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
Western Blot (WB)
(Western blot analysis of ARF1 expression in HEK293 cell lysate (MBS179618).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARF1 monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for ARF1 )
ARF1, Monoclonal Antibody (Cat# AAA179618)
Full Name
Anti-ARF1 Rabbit Monoclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
FC/FACS, IHC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Western Blot (WB)
(Western blot analysis of Mouse, Rat liver lysate showing detection of ~52.7 kDa Presenilin 1 protein using Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-734). Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse liver lysate. Lane 3: Rat liver lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Rabbit Anti-Presenilin 1 Polyclonal Antibody (SPC-734) at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Rabbit HRP:IgG at 1:4000 for 1 hour at RT. Color Development: ECL solution for 5 min at RT. Predicted/Observed Size: ~52.7 kDa. Other Band(s): 28, 32, 42 kDa other isoforms.)
Presenilin 1, Polyclonal Antibody (Cat# AAA8000643)
Full Name
Presenilin 1 Antibody
Gene Names
PSEN1; AD3; FAD; PS1; PS-1; S182; ACNINV3
Reactivity
Mouse; Rat
Applications
WB
Purity
Peptide Affinity Purified
Pricing
$425/0.1 mg | $1,690/5x0.1 mg
Western Blot (WB)
(Western blot analysis of Ras expression in SH-SY5Y cell lysate (MBS179101).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-NRAS monoclonal antibody overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NRAS )
Ras, Monoclonal Antibody (Cat# AAA179101)
Full Name
Anti-Ras Rabbit Monoclonal Antibody
Gene Names
NRAS; NS6; CMNS; NCMS; ALPS4; N-ras; NRAS1
Reactivity
Human, Mouse, Rat
Applications
IP, IF, ICC, WB
Purity
Affinity-chromatography
Pricing
$370/0.1 mL | $1,600/5x0.1 mL
Immunocytochemistry (ICC)
(Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-CD74 Monoclonal Antibody, Clone 1B8 . Tissue: HeLa Cells (Human Cervical Cancer). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-CD74 Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cell membrane, Endoplasmic Reticulum, Golgi apparatus. Magnification: 60X. (A) DAPI (blue) nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) CD74 Antibody (D) Composite.)
CD74, Monoclonal Antibody (Cat# AAA808459)
Full Name
CD74 Antibody, Clone 1B8: Biotin
Gene Names
CD74; II; DHLAG; HLADG; Ia-GAMMA
Reactivity
Human
Applications
WB, ICC, IF
Purity
Protein G Purified
Pricing
$475/0.1 mg | $1,910/5x0.1 mg
