RAB13 blocking peptide

RAB13 Antibody (Center) Blocking Peptide

• Gene Names: RAB13; GIG4
• Synonyms: RAB13; RAB13 Antibody (Center) Blocking Peptide; Ras-related protein Rab-13; Cell growth-inhibiting gene 4 protein; RAB13 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of human RAB13. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 203

• Cellular Location: Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasmic vesicle membrane; Lipid-anchor; Cytoplasmic side. Cell junction, tight junction. Golgi apparatus, trans-Golgi network membrane. Recycling endosome membrane. Cell projection, lamellipodium. Cytoplasmic vesicle, secretory vesicle membrane. Note: Tight junctions or associated with vesicles scattered throughout the cytoplasm in cells lacking tight junctions. Relocalizes to the leading edge of lamellipodia in migrating endothelial cells
• Tissue Location: Detected in several types of epithelia, including intestine, kidney, liver and in endothelial cells
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for RAB13 blocking peptide

The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between an inactive GDP-bound form and an active GTP-bound form that is able to recruit to membranes different sets of downstream effectors directly responsible for vesicle formation, movement, tethering and fusion. That Rab is involved in endocytic recycling and regulates the transport to the plasma membrane of transmembrane proteins like the tight junction protein OCLN/occludin. Thereby, it regulates the assembly and the activity of tight junctions. Moreover, it may also regulate tight junction assembly by activating the PKA signaling pathway and by reorganizing the actin cytoskeleton through the activation of the downstream effectors PRKACA and MICALL2 respectively. Through its role in tight junction assembly, may play a role in the establishment of Sertoli cell barrier. Plays also a role in angiogenesis through regulation of endothelial cells chemotaxis. Also involved in neurite outgrowth. Has also been proposed to play a role in post-Golgi membrane trafficking from the TGN to the recycling endosome. Finally, it has been involved in insulin- induced transport to the plasma membrane of the glucose transporter GLUT4 and therefore may play a role in glucose homeostasis.

NCBI and Uniprot Product Information

• NCBI GI #: 1710016
• NCBI GeneID: 5872
• UniProt Accession #: P51153; Q5U0A6; Q6GPG6; Q96GU4; A8K6B5; D3DV67
• Molecular Weight: 22,774 Da
• NCBI Official Full Name: Ras-related protein Rab-13
• NCBI Official Synonym Full Names: RAB13, member RAS oncogene family
• NCBI Official Symbol: RAB13
• NCBI Official Synonym Symbols: GIG4
• NCBI Protein Information: ras-related protein Rab-13
• UniProt Protein Name: Ras-related protein Rab-13
• Protein Family: Ras-related protein
• UniProt Gene Name: RAB13
• UniProt Entry Name: RAB13_HUMAN

NCBI Description

This gene is a member of the Rab family of small G proteins and plays a role in regulating membrane trafficking between trans-Golgi network (TGN) and recycling endosomes (RE). The encoded protein is involved in the assembly of tight junctions, which are components of the apical junctional complex (AJC) of epithelial cells. The AJC plays a role in forming a barrier between luminal contents and the underlying tissue. Additional functions associated with the protein include endocytic recycling of occludin, regulation of epithelial cell scattering, neuronal regeneration and regulation of neurite outgrowth. Alternately spliced transcript variants have been observed for this gene. A pseudogene associated with this gene is located on chromosome 12. [provided by RefSeq, Jan 2013]

Uniprot Description

RAB13: Could participate in polarized transport, in the assembly and/or the activity of tight junctions. Belongs to the small GTPase superfamily. Rab family.

Protein type: G protein, monomeric, Rab; G protein, monomeric; G protein

Chromosomal Location of Human Ortholog: 1q21.2

Cellular Component: cytoplasm; cytoplasmic vesicle; cytoplasmic vesicle membrane; endocytic vesicle; Golgi apparatus; lamellipodium; lateral plasma membrane; neuron projection; plasma membrane; recycling endosome; tight junction; trans-Golgi network

Molecular Function: GTP binding; GTPase activity; protein binding

Biological Process: cellular response to insulin stimulus; cortical actin cytoskeleton organization and biogenesis; endocytic recycling; endosome transport; neurite development; protein secretion; vesicle docking during exocytosis